Elabhorethri, izinqumo eziqinile ziyenziwa njalo ukuze kunqunywe ukuthi kwenziwa kangcono kanjani ukuhlola nokuhlola okubucayi.Ngokuhamba kwesikhathi, amathiphu e-pipette ajwayele ukuhambisana namalebhu emhlabeni wonke futhi ahlinzeka ngamathuluzi ukuze ochwepheshe nososayensi babe nekhono lokwenza ucwaningo olubalulekile.Lokhu kuyiqiniso ikakhulukazi njengoba i-COVID-19 iqhubeka nokusabalala kulo lonke elase-United States.Izazi ze-Epidemiologists kanye ne-virologist zisebenza ubusuku nemini ukuqhamuka nokwelashwa kwaleli gciwane.Amathiphu ahlungiwe we-pipette enziwe ngamapulasitiki asetshenziselwa ukutadisha leli gciwane futhi amapayipi ayekade eningi, amapayipi engilazi manje asebushelelezi futhi ayazenzakalela.Ingqikithi yamathiphu ayi-10 wamapayipi epulasitiki asetshenziselwa ukwenza ukuhlolwa kwe-COVID-19 okukodwa njengamanje futhi izeluleko eziningi ezisetshenziswayo manje zinezihlungi kuzo okufanele zivimbe u-100% wama-aerosol futhi zivikele ukungcola lapho kwenziwa amasampula.Kodwa angakanani ngempela lawa macebiso abiza kakhulu futhi abiza imvelo azuzisa amalebhu ezweni lonke?Ingabe amalebhu kufanele anqume ukulahla isihlungi?
Ngokuya ngokuhlolwa noma ukuhlolwa okukhona, amalabhorethri nezikhungo zocwaningo zizokhetha ukusebenzisa amathiphu epayipi angahlungiwe noma ahlungiwe.Amalebhu amaningi asebenzisa amathiphu ahlungiwe ngoba akholelwa ukuthi izihlungi zizovimbela wonke ama-aerosol ukuthi angangcolisi isampula.Izihlungi zivame ukubonwa njengendlela eyongayo yokususa ngokuphelele iminonjana yokungcola kusampula, kodwa ngeshwa lokhu akunjalo.Izihlungi zethiphu ye-polyethylene pipette azivimbeli ukungcola, kodwa kunalokho zinciphisa ukusabalala kokungcola.
Isihloko sakamuva se-Biotix sithi, “[igama] elithi isithiyo liyigama elingelona iqiniso kwamanye alawa macebiso.Amathiphu athile kuphela aphezulu anikeza umgoqo wokuvala wangempela.Izihlungi eziningi zinciphisa uketshezi ekungeneni emgqonyeni we-pipette.”Ucwaningo oluzimele lwenziwe kubhekwa ezinye izindlela zezihlungi zamathiphu kanye nempumelelo yazo uma kuqhathaniswa namathiphu angahlungi.I-athikili eyanyatheliswa ku-Journal of Applied Microbiology, e-London (1999) yafunda ukusebenza kahle kwamathiphu okuhlunga i-polyethylene lapho ifakwe ekugcineni kwe-pipettip tip cone yokuvula uma kuqhathaniswa namathiphu angahlungiwe.Ezivivinyweni ezingu-2620, amasampula angu-20% abonise ukungcoliswa kwe-carryover ekhaleni le-pipettor lapho singasetshenziswanga isihlungi, futhi u-14% wamasampuli angcoliswe ngokuphambene lapho kusetshenziswa ithiphu lesihlungi se-polyethylene (PE) (Umfanekiso 2).Ucwaningo luphinde lwathola ukuthi lapho uketshezi lwe-radioactive noma i-plasmid DNA ifakwa ngamapayipi kusetshenziswa isihlungi, ukungcoliswa kombhobho we-pipettor kwenzeka phakathi kwamapayipi ayi-100.Lokhu kubonisa ukuthi nakuba amathiphu ahlungiwe enza ukunciphisa inani lokungcoliswa kwe-cross-contamination kusuka kwelinye ithiphu le-pipette kuya kwelinye, izihlungi azikuyeki ukungcola ngokuphelele.
Isikhathi sokuthumela: Aug-24-2020