Bakeng sa karabelo e atlehileng ea amplification, hoa hlokahala hore likarolo tsa karabelo ka bomong li be teng ka har'a mahloriso a nepahetseng tokisong ka 'ngoe.Ho phaella moo, ke habohlokoa hore ho se be le tšilafalo e hlahang.
Haholo-holo ha mekhoa e mengata e tlameha ho hlophisoa, e thehiloe ho lokisa seo ho thoeng ke motsoako o moholo ho e-na le ho kenya liphaephe tse fapaneng ka har'a sekepe ka seng.Metsoako e lokiselitsoeng esale pele e fumaneha khoebong, eo ho eona ho kenyelletsoang likarolo tse khethehileng tsa sampole (primer) le metsi.Ntle le moo, motsoako oa master o ka lokisoa ke uena.Ka mefuta e 'meli, motsoako o ajoa sekepeng se seng le se seng sa PCR ntle le template 'me sampole ea DNA ka bomong e eketsoa ka thoko qetellong.
Ho sebelisa motsoako o moholo ho na le melemo e 'maloa: Ntlha ea pele, palo ea mehato e le' ngoe ea pipetting e fokotsehile.Ka tsela ena, bobeli ba kotsi ea liphoso tsa basebelisi nakong ea pipetting le kotsi ea tšilafalo e fokotsehile 'me, ha e le hantle, nako e bolokiloe.Ha e le hantle, ho nepahala ha pipetting ho boetse ho phahame, kaha li-volume tse kholo li fokotsoa.Sena se bonolo ho se utloisisa ha u hlahloba lintlha tsa tekheniki tsa li-pipette: Ha molumo o fokotsehile, liphapang li ka ba tse phahameng.Taba ea hore litokisetso tsohle li tsoa sekepeng se le seng se na le phello e ntle ho homogeneity (haeba e kopane hantle).Sena se boetse se ntlafatsa ho ikatisa ha liteko.
Ha u lokisetsa motsoako o mongata, bonyane 10% ea molumo o eketsehileng o lokela ho eketsoa (mohlala, haeba ho hlokahala litokisetso tse 10, bala motheong oa 11), e le hore esita le sekepe sa ho qetela se tlale hantle.Ka tsela ena, (hanyenyane) ho se nepahale ha lipeipi, le phello ea tahlehelo ea sampole ha litharollo tse nang le li-detergent li ka lefshoa.Li-detergents li fumaneha ka har'a litharollo tsa enzyme tse kang polymerase le li-mix mixes, tse bakang foam le masala holim'a bokahare ba lintho tse tloaelehileng.malebela a pipette.
Ho ipapisitsoe le ts'ebeliso le mofuta oa mokelikeli o tla fanoa, ho lokela ho khethoa mokhoa o nepahetseng oa liphaephe (1) mme ho khethoe lisebelisoa tse nepahetseng.Bakeng sa litharollo tse nang le li-detergents, ho khothalletsoa mokhoa o tobileng oa ho falla kapa seo ho thoeng ke "poloko e tlaase" malebela a pipette e le mokhoa o mong oa li-pipette tsa moea-cushion.Phello eaKeletso ea ACE PIPETTEe thehiloe holim'a sebaka se khethehileng sa hydrophobic.Mekelikeli e nang le li-detergents ha e tlohele filimi e setseng ka hare le ka ntle, e le hore tahlehelo ea tharollo e fokotsehe.
Ntle le dosing e nepahetseng ea likarolo tsohle, ke habohlokoa hape hore ho se be le tšilafalo ea litokisetso e hlahang.Ha hoa lekana ho sebelisa lisebelisoa tsa bohloeki bo phahameng, hobane mokhoa oa pipetting ka pipette ea moea o ka hlahisa li-aerosol tse setseng ka pipette.DNA e ka 'nang ea e-ba teng ka har'a aerosol e ka fetisetsoa ho tloha sampuling e' ngoe ho ea ho e 'ngoe ka mohato o latelang oa pipetting' me kahoo ea lebisa tšilafalo.Litsamaiso tse tobileng tsa phalliso tse boletsoeng ka holimo le tsona li ka fokotsa kotsi ena.Bakeng sa li-pipette tsa moea-cushion hoa utloahala ho sebelisa malebela a sefa ho sireletsa khoune ea pipette ka ho boloka li-splashes, li-aerosols le li-biomolecules.
Nako ea poso: Dec-06-2022