V8 RNA-seq Library Prep Kit for RNA Library Construction: Loaʻa i kahi ʻōlelo, nīnau, kumu kūʻai a kūʻai paha.

Hoʻohana mākou i nā kuki e hoʻonui i kou ʻike. Ma ka hoʻomau ʻana i ka nānā ʻana i kēia pūnaewele e ʻae ʻoe i kā mākou hoʻohana ʻana i nā kuki. ʻIke hou aku.
Hoʻohana ʻia ka VAHTS Universal V8 RNA-seq Library Prep Kit no Illumina no ka hoʻomākaukau ʻana i nā hale waihona puke transcriptome no nā kahua Illumina.
Aia i loko o ka pahu nā ʻano ʻelua cDNA 2nd Strand synthesis buffers. Hiki ke koho ʻia nā buffers e kūkulu i nā hale waihona puke no ka kānana transcriptome RNA-Seq i hoʻopaʻa ʻole ʻia a i ʻole. kaʻina hana hale waihona puke a hōʻemi i ka manawa hana.
Hoʻomaikaʻi ka ʻōnaehana hopena i hoʻomaikaʻi ʻia i ka hana hale waihona puke, hāʻawi i ka uhi ʻokoʻa no nā helu like ʻole o ka RNA hoʻokomo, a kūpono me nā RNA hoʻokomo haʻahaʻa. pili nā buffer i ka mana koʻikoʻi koʻikoʻi a me ka hoʻāʻo hana no ka paʻa maikaʻi a me ka hana hou.
Hiki ke hoʻohana ʻia ka VAHTS Universal V8 RNA-seq Library Prep Kit for Illumina no ke kūkulu ʻana i ka poly(A)-enriched total RNA (no ka RNA o ka kūpaʻa maikaʻi mai nā eukaryotes e like me nā mea kanu, holoholona, ​​a me nā haʻuki) a i ʻole ka hoʻopau ʻana o ka rRNA RNA hale waihona puke. No ka mea ʻokoʻa loa ka ʻike mRNA o ka RNA holoʻokoʻa i nā laʻana like ʻole, pono e hoʻokomo i ka nui o ka RNA āpau e hōʻoia i ka lawa o ka mRNA no ke kūkulu hale waihona.
Ribo-off rRNA Depletion Kit (Human/Mouse/Rat) (Vazyme #N406): 0.01–1 g;VAHTS mRNA Hopu (Vazyme #N401): 0.01–4 g;Ribo-off rRNA Depletion Kit (Bacteria) (Vazyme #N407): 0.01–5 g;Ribo-off rRNA Depletion Kit (mea kanu) (Vazyme #N409): 1-5 g;Ribo-off Globin & rRNA Depletion Kit (kanaka/iole/iole) (Vazyme #N408): 0.01–1 g;mRNA i hoʻomaʻemaʻe ʻia a i ʻole ribosome RNA pau ʻole: 0.5–100 ng.
ʻOi aku ka maikaʻi o ka hoʻohana ʻana i ka Agilent 2100 Bioanalyzer e aʻo i ka pono o ka huina RNA.mRNA i hoʻohui ʻia me VAHTS mRNA Capture Beads (Vazyme #N401) he RNA kiʻekiʻe (RIN 7), no ka mea, hiki i ka RNA hoʻohaʻahaʻa i hoʻohana ʻia no ka hana hale waihona puke ke kumu 3 ' bias ma RNA-seq. No nā laʻana RNA me nā waiwai RIN <7, kūpono ke ʻano Ribo-off (Vazyme #N406/407/408/409) no ka wehe ʻana i ka rRNA.
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