V8 RNA-seq Cov Tsev Qiv Ntawv Npaj rau RNA Tsev Qiv Ntawv Kev Tsim Kho: Tau Txais Cov Lus Qhia, Nug, Nqe lossis Yuav

Peb siv cov ncuav qab zib los txhim kho koj cov kev paub dhau los.Los ntawm kev txuas ntxiv mus rau qhov chaw no koj pom zoo rau peb siv cov ncuav qab zib.Ntau cov ntaub ntawv.
Lub VAHTS Universal V8 RNA-seq Library Prep Kit rau Illumina yog siv los npaj cov tsev qiv ntawv transcriptome rau Illumina platforms.Cov khoom siv muaj ntau yam thiab tsim nyog rau RNA tsev qiv ntawv kev tsim kho ntawm RNA tau los ntawm Poly(A)-based mRNA enrichment lossis rRNA depletion.
Cov khoom siv muaj ob hom cDNA 2nd Strand synthesis buffers.Buffers tuaj yeem xaiv los tsim cov tsev qiv ntawv rau stranded los yog unstranded RNA-Seq transcriptome tsom xam.Cov khoom siv integrates 2nd Strand cDNA synthesis, dA-Tailing, thiab kawg kho yam tsis muaj purification, uas yooj yim rau cov khoom siv. cov txheej txheem tsim tsev qiv ntawv thiab txo lub sijhawm ua haujlwm.
Cov tshuaj tiv thaiv zoo tshaj plaws txhim kho cov tsev qiv ntawv kev tsim kho kom zoo, muab cov kev pab cuam sib txawv rau ntau qhov sib txawv ntawm cov tswv yim RNA, thiab sib haum nrog cov tswv yim qis RNAs.Tom qab xaiv qhov loj me siv cov hlaws sib nqus, cov tsev qiv ntawv kev cai ntawm ib qhov loj me tuaj yeem tau.Cov khoom siv cov enzymes thiab buffers yog raug rau kev tswj kom zoo thiab kev sim ua haujlwm kom pom kev ruaj ntseg thiab rov tsim dua tshiab.
Lub VAHTS Universal V8 RNA-seq Library Prep Kit rau Illumina tuaj yeem siv los tsim cov poly(A)-enriched tag nrho RNA (rau RNA ntawm kev ncaj ncees los ntawm eukaryotes xws li nroj tsuag, tsiaj txhu, thiab fungi) los yog rRNA depletion RNA tsev qiv ntawv siv. Txij li thaum mRNA cov ntsiab lus ntawm tag nrho RNA nyob rau hauv cov qauv sib txawv sib txawv heev, nws yog ib qho tsim nyog yuav tsum tau nkag siab tag nrho RNA kom txaus mRNA rau kev tsim tsev qiv ntawv.
Ribo-off rRNA Depletion Kit (Tib neeg / Nas / Rat) (Vazyme #N406): 0.01–1 g;VAHTS mRNA Capture Beads (Vazyme #N401): 0.01–4 g;Ribo-off rRNA Depletion Kit (Bacteria) (Vazyme #N407): 0.01–5 g;Ribo-off rRNA Depletion Kit (cog) (Vazyme #N409): 1-5 g;Ribo-off Globin & rRNA Depletion Kit (tib neeg / nas / nas) (Vazyme #N408): 0.01–1 g;purified mRNA los yog ribosome depleted RNA: 0.5-100 ng.
Nws yog qhov zoo tshaj plaws los siv Agilent 2100 Bioanalyzer los kawm txog kev ncaj ncees ntawm tag nrho RNA.mRNA ntxiv nrog VAHTS mRNA Capture Beads (Vazyme #N401) yuav tsum zoo RNA (RIN 7), raws li degraded tag nrho RNA siv rau kev tsim tsev qiv ntawv tuaj yeem ua rau 3 ' bias hauv RNA-seq.Rau cov qauv RNA nrog RIN qhov tseem ceeb <7, Ribo-off txoj kev (Vazyme #N406/407/408/409) yog tsim rau rRNA tshem tawm.
Cov khoom thiab cov kev pab cuam no yog muab rau koj yooj yim.News-Medical.Net tsis muaj txoj kev pom zoo ib yam ntawm cov khoom thiab cov kev pab cuam.