V8 RNA-seq Isomero Gutegura Igitabo Kubaka Isomero rya RNA: Kubona Amagambo, Kubaza, Igiciro cyangwa Kugura

Dukoresha kuki kugirango tuzamure uburambe.Mu gukomeza gushakisha kururu rubuga wemera gukoresha kuki. Andi makuru.
VAHTS Universal V8 RNA-seq Isomero Gutegura Isomero rya Illumina rikoreshwa mugutegura amasomero yanditsemo inyandiko za platform ya Illumina.Igitabo kirahuzagurika kandi gikwiranye no kubaka isomero rya RNA ryubatswe na RNA yakiriwe na Poly (A) ishingiye kuri mRNA cyangwa gutunga rRNA.
Igikoresho kirimo ubwoko bubiri bwa cDNA ya 2 ya Syndhesis ya buffer.Buffers irashobora gutoranywa kugirango yubake amasomero yo gusesengura inyandiko ya RNA-Seq idahagaritswe. inzira yo kubaka isomero kandi igabanya igihe cyo gukora.
Sisitemu yuburyo bwiza bwo kunoza imikorere yububiko bwububiko bwibitabo, itanga ubwishingizi bumwe kubwinshi bwinjiza RNA, kandi irahuza na RNA yo hasi. Nyuma yo guhitamo ingano ukoresheje amasaro ya magneti, isomero ryihariye ryubunini bwihariye rirashobora kuboneka. Enzymes za kit na buffers zigenzurwa nubuziranenge bukomeye hamwe nigeragezwa ryimikorere kugirango ituze neza kandi yororoke.
VAHTS Universal V8 RNA-seq Isomero Gutegura Isomero rya Illumina rirashobora gukoreshwa mukubaka poli (A) ikungahaye kuri RNA yose (kuri RNA yubunyangamugayo bwiza buturuka kuri eukaryote nkibimera, inyamaswa, nibihumyo) cyangwa kubaka isomero rya RNA. Kubera ko mRNA yibirimo RNA yose mubitegererezo bitandukanye biratandukanye cyane, birakenewe ko winjiza RNA ihagije kugirango tumenye mRNA ihagije yo kubaka isomero.
Ribo-off rRNA Igikoresho cyo Kumanura (Umuntu / Imbeba / Imbeba) (Vazyme # N406): 0.01–1 g;VAHTS mRNA Gufata Amashapure (Vazyme # N401): 0.01–4 g;Ribo-off rRNA Igikoresho cyo Kugabanuka (Bacteria) (Vazyme # N407): 0.01–5 g;Ribo-off rRNA Igikoresho cyo Kuzimya (igihingwa) (Vazyme # N409): 1-5 g;Ribo-off Globin & rRNA Igikoresho cyo Kumanura (umuntu / imbeba / imbeba) (Vazyme # N408): 0.01–1 g;mRNA yatunganijwe cyangwa ribosome yagabanutse RNA: 0.5-100 ng.
Nibyiza gukoresha Agilent 2100 Bioanalyser kugirango wige ubunyangamugayo bwa RNA.mRNA yunganirwa na VAHTS mRNA Ifata Amashara (Vazyme # N401) igomba kuba yujuje ubuziranenge RNA (RIN 7), kuko RNA yangiritse yose ikoreshwa mukubaka amasomero irashobora gutera 3 Kubogama muri RNA-seq. Kuburugero rwa RNA hamwe nagaciro ka RIN <7, uburyo bwa Ribo-off (Vazyme # N406 / 407/408/409) burakwiriye gukuraho RRNA.
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