Isu tinoshandisa makuki kuwedzera ruzivo rwako.Nekuramba uchitsvaga saiti ino unobvuma kushandisa kwedu makuki.Rumwe ruzivo.
VAHTS Universal V8 RNA-seq Library Prep Kit yeIllumina inoshandiswa kugadzira mabhuku ezvinyorwa zveIllumina mapuratifomu.Iyo kit inoshandiswa zvakasiyana-siyana uye yakakodzera RNA raibhurari yekuvaka yeRNA yakawanikwa nePoly (A)-based mRNA enrichment kana rRNA kupera.
Iyo kit ine mhando mbiri dze cDNA 2nd Strand synthesis buffers. Mabuffers anogona kusarudzwa kuvaka maraibhurari eRNA-Seq transcriptome analysis. Raibhurari yekuvaka maitiro uye inoderedza nguva yekushanda.
Iyo optimized reaction system inovandudza hunyanzvi hwekuvaka raibhurari, inopa kufukidzwa kwakafanana kwehuwandu hwakasiyana hwekupinza RNA, uye inoenderana neyakaderera yekuisa RNAs.Mushure mekusarudza saizi uchishandisa magineti bead, raibhurari yetsika yehukuru hwakati inogona kuwanikwa.The kit's enzymes uye mabuffers ari pasi pekuomesera kwemhando yekudzora uye inoshanda kuyedzwa kwekunyatsogadzikana uye kuberekana.
Iyo VAHTS Universal V8 RNA-seq Raibhurari Yekugadzirira Kit yeIllumina inogona kushandiswa kuvaka ingave poly(A) -inopfumisa yakazara RNA (yeRNA yeruvimbiko rwakanaka kubva kueukaryotes senge zvidyarwa, mhuka, uye fungi) kana rRNA kupera RNA raibhurari kuvaka. Sezvo iyo mRNA yemukati yeRNA yakazara mumasampuli akasiyana ichisiyana zvakanyanya, zvinodikanwa kuisa yakakwana yakakwana RNA kuti ive nechokwadi chemRNA yakakwana yekuvaka raibhurari.
Ribo-off rRNA Depletion Kit (Munhu / Mouse / Rat) (Vazyme #N406): 0.01-1 g;VAHTS mRNA Capture Beads (Vazyme #N401): 0.01–4 g;Ribo-off rRNA Depletion Kit (Bacteria) (Vazyme #N407): 0.01–5 g;Ribo-off rRNA Depletion Kit (chirimwa) (Vazyme #N409): 1-5 g;Ribo-off Globin & rRNA Depletion Kit (munhu/mbeva/gonzo) (Vazyme #N408): 0.01–1 g;yakanatswa mRNA kana ribosome yakapera RNA: 0.5-100 ng.
Zvakanakisisa kushandisa Agilent 2100 Bioanalyzer kuti udzidze kutendeseka kweRNA.mRNA yakawedzerwa neVAHTS mRNA Capture Beads (Vazyme #N401) inofanira kunge iri yepamusoro-soro RNA (RIN 7), sezvo yakasvibiswa yakazara RNA inoshandiswa pakuvaka raibhurari inogona kukonzera 3. ' bias muRNA-seq.Kune RNA samples ine RIN kukosha <7, iyo Ribo-off nzira (Vazyme #N406/407/408/409) yakakodzera rRNA kubviswa.
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Nguva yekutumira: Chivabvu-06-2022