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VAHTS Universal V8 RNA-seq Apo Prepu Library fun Illumina ni a lo lati ṣeto awọn ile-ikawe transcriptome fun awọn iru ẹrọ Illumina. Ohun elo naa wapọ ati pe o dara fun ikole ile-ikawe RNA ti RNA ti o gba nipasẹ Poly(A) -orisun mRNA imudara tabi idinku rRNA.
Ohun elo naa ni awọn oriṣi meji ti cDNA 2nd Strand synthes buffers. Awọn buffers le jẹ yan lati kọ awọn ile-ikawe fun iyasọtọ tabi itupalẹ transcriptome RNA-Seq ti ko ni iyasọtọ. Ohun elo naa ṣepọpọ 2nd Strand cDNA synthesis, dA-Tailing, ati atunṣe ipari laisi iwẹnumọ, eyiti o jẹ irọrun simplifies awọn ìkàwé ikole ilana ati ki o din isẹ akoko.
Awọn iṣapeye lenu eto se ìkàwé ikole ṣiṣe, pese aṣọ agbegbe fun yatọ si oye ti input RNA, ati ki o jẹ ibamu pẹlu kekere input RNAs.After yiyan a iwọn lilo awọn se ilẹkẹ, a aṣa ìkàwé ti kan pato iwọn le ti wa ni obtain.The kit ká ensaemusi ati awọn buffers wa labẹ iṣakoso didara lile ati idanwo iṣẹ-ṣiṣe fun iduroṣinṣin to dara julọ ati atunṣe.
VAHTS Universal V8 RNA-seq Apo Prepu Library fun Illumina le ṣee lo lati kọ boya poly(A) ti o ni imudara lapapọ RNA (fun RNA ti iduroṣinṣin to dara lati awọn eukaryotes gẹgẹbi awọn ohun ọgbin, ẹranko, ati elu) tabi ikole ikawe RNA idinku rRNA. Niwọn igba ti akoonu mRNA ti lapapọ RNA ni awọn ayẹwo oriṣiriṣi yatọ pupọ, o jẹ dandan lati tẹ RNA lapapọ sii lati rii daju mRNA to fun ikole ile-ikawe.
Ribo-pipa rRNA Apo Irẹwẹsi (Eniyan / Asin / Eku) (Vazyme # N406): 0.01-1 g;Awọn Ilẹkẹ Yaworan VAHTS mRNA (Vazyme # N401): 0.01–4 g;Ribo-pipa rRNA Idinku Apo (Bacteria) (Vazyme # N407): 0.01-5 g;Ribo-pipa rRNA Idinku Apo (ọgbin) (Vazyme # N409): 1-5 g;Ribo-pa Globin & rRNA Idinku Apo (eda eniyan / Asin / eku) (Vazyme # N408): 0.01-1 g;mRNA ti a wẹ tabi ribosome dinku RNA: 0.5-100 ng.
O dara julọ lati lo Agilent 2100 Bioanalyzer lati ṣe iwadi iduroṣinṣin ti lapapọ RNA.mRNA ti o ni afikun pẹlu VAHTS mRNA Capture Beads (Vazyme #N401) yẹ ki o jẹ RNA ti o ni agbara giga (RIN 7), nitori ibajẹ lapapọ RNA ti a lo fun ikole ile-ikawe le fa 3 "Irẹwẹsi ni RNA-seq. Fun awọn ayẹwo RNA pẹlu awọn iye RIN <7, ọna Ribo-off (Vazyme #N406/407/408/409) dara fun yiyọkuro rRNA.
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Akoko ifiweranṣẹ: May-06-2022