V8 RNA-seq Library Prep Kit mo le Fausiaina o le Faletusi RNA: Maua se Fa'amatalaga, Su'esu'ega, Tau po'o le Fa'atau

Matou te faʻaogaina kuki e faʻaleleia ai lou poto masani. E ala i le faʻaauauina o le suʻesuʻeina o lenei 'upega tafaʻilagi e te ioe i le matou faʻaogaina o kuki. Faʻamatalaga atili.
O le VAHTS Universal V8 RNA-seq Library Prep Kit mo Illumina o loʻo faʻaaogaina e saunia ai faletusi transcriptome mo Illumina platforms.O le pusa e faʻaogaina ma talafeagai mo le fausiaina o le faletusi RNA o le RNA maua e le Poly (A) -faʻavae mRNA faʻatamaoaigaina poʻo le faʻaitiitia o le rRNA.
O le pusa o loʻo i ai ituaiga e lua o le cDNA 2nd Strand synthesis buffers. E mafai ona filifilia faʻamau e fausia ai faletusi mo le RNA-Seq suʻesuʻega faʻasalalau pe leai foi. faiga fau faletusi ma fa'aitiitia le taimi fa'atino.
O le faiga tali sili ona lelei e faʻaleleia atili ai le fausiaina o faletusi, e tuʻuina atu ai le toniga mo le aofaʻi eseese o RNA faʻapipiʻi, ma e fetaui ma RNA faʻapipiʻi maualalo. A maeʻa ona filifilia se tele e faʻaaoga ai pulu maneta, e mafai ona maua se faletusi faʻapitoa o se lapoʻa faʻapitoa. pa'u e fa'atatau i le fa'atonutonuina lelei ma le su'ega fa'atino mo le mautu lelei ma le toe gaosia.
O le VAHTS Universal V8 RNA-seq Library Prep Kit mo Illumina e mafai ona fa'aogaina e fausia ai le poly(A)-fa'a'oa'oa atoa RNA (mo le RNA o le amio sa'o lelei mai eukaryotes e pei o la'au, manu, ma sigi) po'o le fa'aleagaina o le rRNA faletusi RNA. Talu ai o le mRNA o loʻo i totonu o le RNA atoa i faʻataʻitaʻiga eseese e matua ese lava, e manaʻomia le tuʻuina atu o le RNA atoa e faʻamautinoa ai le lava mRNA mo le fausiaina o faletusi.
Ribo-off rRNA Depletion Kit (Tagata/Iosu/Iole) (Vazyme #N406): 0.01–1 g;VAHTS mRNA Pu'e Lopa (Vazyme #N401): 0.01–4 g;Ribo-off rRNA Depletion Kit (Bacteria) (Vazyme #N407): 0.01-5 g;Ribo-off rRNA Depletion Kit (la'au) (Vazyme #N409): 1–5 g;Ribo-off Globin & rRNA Depletion Kit (tagata/ isumu/iole) (Vazyme #N408): 0.01–1 g;mRNA fa'amama po'o le ribosome RNA fa'aumatia: 0.5-100 ng.
E sili ona lelei le faʻaaogaina o le Agilent 2100 Bioanalyzer e suʻesuʻe ai le faʻamaoni o le RNA atoa.mRNA faʻaopoopoina ma VAHTS mRNA Capture Beads (Vazyme #N401) e tatau ona maualuga le RNA (RIN 7), ona o le RNA faʻaleagaina atoa e faʻaaogaina mo le fausiaina o faletusi e mafai ona mafua ai le 3 ' fa'aituau i le RNA-seq. Mo fa'ata'ita'iga RNA fa'atasi ma tau RIN <7, o le Ribo-off method (Vazyme #N406/407/408/409) e talafeagai mo le aveeseina o rRNA.
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