V8 RNA-seq Prep Kit don Gina Laburaren RNA: Sami Quote, Bincike, Farashi ko Siya

Muna amfani da kukis don haɓaka ƙwarewar ku.Ta ci gaba da bincika wannan rukunin yanar gizon kun yarda da amfani da kukis.Ƙarin bayani.
Ana amfani da Kit ɗin Prep Library na VAHTS Universal V8 RNA-seq don Illumina don shirya dakunan karatu don dandamali na Illumina.Kit ɗin yana da yawa kuma ya dace da ginin ɗakin karatu na RNA na RNA da Poly(A) ya samu ta haɓakar mRNA ta tushen mRNA ko ragewar rRNA.
Kit ɗin ya ƙunshi nau'i biyu na cDNA 2nd Strand synthes buffers. Ana iya zaɓar masu buffers don gina ɗakunan karatu don nazarin RNA-Seq mai raɗaɗi ko mara tushe. Kayan yana haɗa haɗin cDNA na biyu Strand, dA-Tailing, da kuma ƙare gyara ba tare da tsarkakewa ba, wanda ke sauƙaƙe tsarin gina ɗakin karatu kuma yana rage lokacin aiki.
Ingantattun tsarin amsawa yana inganta ingantaccen ginin ɗakin karatu, yana ba da ɗaukar hoto don nau'ikan shigar da RNA daban-daban, kuma yana dacewa da ƙananan shigarwar RNAs.Bayan zaɓin girman ta amfani da beads na maganadisu, ana iya samun ɗakin karatu na al'ada na takamaiman girman. buffers suna ƙarƙashin kulawar inganci mai ƙarfi da gwajin aiki don ingantacciyar kwanciyar hankali da haɓakawa.
Za a iya amfani da Kit ɗin Prep Library na VAHTS Universal V8 RNA-seq don Illumina don gina ko dai poly(A) - wadatar jimillar RNA (don RNA na ingantaccen mutunci daga eukaryotes kamar tsire-tsire, dabbobi, da fungi) ko ginin ɗakin karatu na rRNA. Tunda abun ciki na mRNA na jimillar RNA a cikin samfura daban-daban ya bambanta sosai, wajibi ne a shigar da isassun RNA gaba ɗaya don tabbatar da isassun mRNA don ginin ɗakin karatu.
Ribo-off rRNA Kit ɗin Ragewa (Mutum/Mouse/Bera) (Vazyme #N406): 0.01-1 g;VAHTS mRNA Kama Beads (Vazyme #N401): 0.01-4 g;Ribo-off rRNA Depletion Kit (Bacteria) (Vazyme #N407): 0.01-5 g;Ribo-off rRNA Depletion Kit (shuka) (Vazyme #N409): 1-5 g;Ribo-off Globin & rRNA Depletion Kit (mutum/ linzamin kwamfuta/bera) (Vazyme #N408): 0.01–1 g;mRNA mai tsabta ko ribosome da aka rage RNA: 0.5-100 ng.
Zai fi kyau a yi amfani da Agilent 2100 Bioanalyzer don nazarin amincin jimillar RNA.mRNA da aka haɓaka tare da VAHTS mRNA Capture Beads (Vazyme #N401) yakamata ya zama RNA mai inganci (RIN 7), kamar yadda ƙarancin RNA da aka yi amfani da shi don ginin ɗakin karatu na iya haifar da 3. ' son zuciya a cikin RNA-seq. Don samfuran RNA tare da ƙimar RIN <7, hanyar Ribo-off (Vazyme # N406/407/408/409) ya dace da cire rRNA.
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