V8 RNA-seq Qalabka Diyaarinta Maktabada ee Dhismaha Maktabada RNA: Hel Xigasho, Weydiin, Qiimo ama Iibso

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Qalabka udiyaarinta maktabada VAHTS Universal V8 RNA-seq ee Illumina waxaa loo isticmaalaa in lagu diyaariyo maktabadaha transcriptome ee aaladaha Illumina.Qalabka waa mid badan oo ku habboon dhismaha maktabadda RNA ee RNA oo ay heshay Poly(A) kobcinta mRNA-ku-saleysan ama dhimista rRNA.
Qalabku waxa uu ka kooban yahay laba nooc oo cDNA 2nd Strand synthesis buffers.Buffers waxa loo dooran karaa in ay dhisaan maktabado si ay u falanqeeyaan RNA-Seq transcriptome go'doonsan ama aan xidhnayn Habka dhismaha maktabadda waxayna yaraynaysaa wakhtiga hawlgalka.
Nidaamka falcelinta ee la hagaajiyay wuxuu hagaajiyaa waxtarka dhismaha maktabadda, wuxuu bixiyaa dabool isku mid ah oo loogu talagalay xaddi kala duwan oo RNA ah, wuxuuna la jaan qaadayaa RNA-yada hoose ee gelinta kaydiyayaashu waxay ku xiran yihiin xakamaynta tayada adag iyo tijaabinta shaqaynta ee xasiloonida ugu fiican iyo soo saarista
Qalabka udiyaarinta maktabadda VAHTS Universal V8 RNA-seq ee Illumina waxaa loo isticmaali karaa in lagu dhiso wadarta guud ee RNA (A) tayeysan (RNA ee daacadnimada wanaagsan ee eukaryotes sida dhirta, xayawaanka, iyo fangaska) ama rRNA dhismaha maktabadda RNA. Maadaama maadada mRNA ee wadarta RNA ee muunado kala duwan ay aad u kala duwan yihiin, waa lagama maarmaan in la geliyo wadarta guud ee RNA si loo hubiyo in mRNA ku filan dhismaha maktabadda.
Ribo-off rRNA Xirmada baabi'inta (Human/Mouse/Jiir) (Vazyme #N406): 0.01-1 g;VAHTS mRNA Qabashada Kuul (Vazyme #N401): 0.01-4 g;Ribo-off rRNA Kit depletion Kit (Bacteria) (Vazyme #N407): 0.01-5 g;Ribo-off rRNA Kit depletion (dhirta) (Vazyme #N409): 1-5 g;Ribo-off Globin & rRNA Qalabka baabi'inta (bini'aadamka / jiirka / jiirka) (Vazyme # N408): 0.01-1 g;MRNA la safeeyey ama RNA ribosome oo daciifay: 0.5-100 ng.
Waxaa ugu wanaagsan in la isticmaalo Agilent 2100 Bioanalyzer si loo barto daacadnimada wadarta guud ee RNA.mRNA oo lagu kabo VAHTS mRNA Capture Beads (Vazyme #N401) waa inay ahaataa RNA tayo sare leh (RIN 7), maadaama wadarta guud ee RNA ee loo isticmaalo dhismaha maktabadda ay sababi karto 3 Eex ku jirta RNA-seq.Samballada RNA ee leh qiyamka RIN<7, habka Ribo-off (Vazyme #N406/407/408/409) ayaa ku habboon ka saarida rRNA.
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