V8 RNA-seq Kit Prep Library maka Mwube Ọbá akwụkwọ RNA: Nweta Quote, Ajụjụ, Ọnụ ahịa ma ọ bụ Zụrụ

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A na-eji VAHTS Universal V8 RNA-seq Library Prep Kit maka Illumina na-akwadebe ọba akwụkwọ transcriptome maka nyiwe Illumina. Ngwa ahụ dị mma ma dabara adaba maka owuwu ọba akwụkwọ RNA nke RNA nwetara site na nkwalite mRNA dabere na Poly(A).
Ngwa ahụ nwere ụdị abụọ nke cDNA 2nd Strand synthes buffers. Enwere ike ịhọrọ ndị na-ebufe ka ha wuo ụlọ akwụkwọ maka nyocha transcriptome RNA-Seq nke agbachiri ma ọ bụ nke na-adịghị agwụ agwụ. Ngwa ahụ na-ejikọta 2nd Strand cDNA synthesis, dA-Tailing, na nkwụsị nkwụsị na-enweghị ọcha, nke na-eme ka ọ dị mfe. usoro iwu ụlọ akwụkwọ ma na-ebelata oge ọrụ.
The kacha mmeghachi omume usoro mma n'ọbá akwụkwọ na-ewu arụmọrụ, na-enye otu mkpuchi maka dị iche iche nke ntinye RNA, na dakọtara na ala ntinye RNAs.Mgbe ahọpụtara size site na iji magnetik beads, a omenala ọbá akwụkwọ nke a kpọmkwem size nwere ike nweta.The kit si enzymes na. buffers nọ n'okpuru njikwa mma siri ike yana nnwale arụ ọrụ maka nkwụsi ike kacha mma na mmụgharị.
Enwere ike iji VAHTS Universal V8 RNA-seq Library Prep Kit maka Illumina wuo ma ọ bụ poly(A) nwere RNA zuru oke (maka RNA nke ezigbo iguzosi ike n'ezi ihe sitere na eukaryotes dị ka osisi, anụmanụ, na fungi) ma ọ bụ rRNA depletion owuwu ọba akwụkwọ RNA. Ebe ọ bụ na ọdịnaya mRNA nke mkpokọta RNA dị na nlele dị iche iche dịgasị iche iche, ọ dị mkpa itinye RNA zuru ezu iji hụ na mRNA zuru ezu maka iwu ụlọ ọba akwụkwọ.
Ribo-off rRNA Ngwunye Ngwunye (mmadụ / òké / oke) (Vazyme #N406): 0.01-1 g;VAHTS mRNA Capture Beads (Vazyme #N401): 0.01–4 g;Ribo-off rRNA Ngwunye Ngwunye (Bacteria) (Vazyme #N407): 0.01-5 g;Ribo-off rRNA Mpempe Ngwunye (osisi) (Vazyme #N409): 1-5 g;Ribo-off Globin & rRNA Mpempe Mmebi (mmadụ/òké/oke) (Vazyme #N408): 0.01–1 g;mRNA dị ọcha ma ọ bụ RNA ribosome mebiri emebi: 0.5-100 ng.
Ọ kachasị mma iji Agilent 2100 Bioanalyzer iji mụọ iguzosi ike n'ezi ihe nke mkpokọta RNA.mRNA agbakwunyere na VAHTS mRNA Capture Beads (Vazyme #N401) kwesịrị ịbụ RNA dị elu (RIN 7), n'ihi na mkpokọta RNA mebiri emebi ejiri rụọ ụlọ ọba akwụkwọ nwere ike ibute 3. Maka ihe nlele RNA nwere ụkpụrụ RIN <7, usoro Ribo-off (Vazyme #N406/407/408/409) dabara maka iwepụ rRNA.
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