Re sebelisa li-cookies ho ntlafatsa boiphihlelo ba hau.Ka ho tsoela pele ho bala sebaka sena sa marang-rang, u lumellana le tšebeliso ea rona ea li-cookies.Boitsebiso bo bong.
The VAHTS Universal V8 RNA-seq Library Prep Kit bakeng sa Illumina e sebelisetsoa ho lokisa lilaebrari tsa transcriptome bakeng sa li-platform tsa Illumina. The kit e na le mekhoa e mengata e mengata 'me e loketse RNA laebrari kaho ea RNA e fumanoang ke Poly(A)-based mRNA enrichment kapa rRNA depletion.
The kit e na le mefuta e 'meli ea cDNA 2nd Strand synthesis buffers. Li-buffers li ka khethoa ho haha lilaeborari bakeng sa tlhahlobo ea transcriptome ea stranded kapa e sa tsitsang ea RNA-Seq. The kit e kopanya 2nd Strand cDNA synthesis, dA-Tailing, le ho lokisa ho qetela ntle le tlhoekiso, e leng ho nolofatsang mokhoa oa kaho ea laebrari le ho fokotsa nako ea ts'ebetso.
Sistimi e ntlafalitsoeng ea maikutlo e ntlafatsa katleho ea kaho ea laeborari, e fana ka tšireletso e ts'oanang bakeng sa litekanyo tse fapaneng tsa ho kenya RNA, 'me e tsamaisana le li-RNA tse kenang ka tlase. Kamora ho khetha boholo bo sebelisang lifaha tsa makenete, ho ka fumanoa laebrari ea boholo bo itseng.Li-enzyme tsa kit le li-buffers li tlas'a taolo e matla ea boleng le tlhahlobo ea ts'ebetso bakeng sa botsitso bo nepahetseng le ho ikatisa.
VAHTS Universal V8 RNA-seq Library Prep Kit bakeng sa Illumina e ka sebelisoa ho haha poly(A)-enriched total RNA (bakeng sa RNA ea botšepehi bo botle ho tsoa ho eukaryote e kang limela, liphoofolo le li-fungus) kapa rRNA depletion laeborari ea RNA kaho. Kaha litaba tsa mRNA tsa kakaretso ea RNA ka lisampole tse fapaneng li fapana haholo, hoa hlokahala ho kenya RNA e lekaneng ho netefatsa mRNA e lekaneng bakeng sa kaho ea laeborari.
Ribo-off rRNA Depletion Kit (Motho / Mouse / Rat) (Vazyme #N406): 0.01-1 g;VAHTS mRNA Capture Beads (Vazyme #N401): 0.01–4 g;Ribo-off rRNA Depletion Kit (Bacteria) (Vazyme #N407): 0.01-5 g;Ribo-off rRNA Depletion Kit (semela) (Vazyme #N409): 1-5 g;Ribo-off Globin & rRNA Depletion Kit (motho / mouse / rat) (Vazyme #N408): 0.01-1 g;mRNA e hloekisitsoeng kapa ribosome e senyehileng ea RNA: 0.5-100 ng.
Ho molemo ho sebelisa Agilent 2100 Bioanalyzer ho ithuta botšepehi ba RNA.mRNA eohle e tlatsitsoeng ka VAHTS mRNA Capture Beads (Vazyme #N401) e lokela ho ba RNA ea boleng bo phahameng (RIN 7), kaha RNA eohle e senyehileng e sebelisetsoang kaho ea laebrari e ka baka 3. ' bias ho RNA-seq. Bakeng sa lisampole tsa RNA tse nang le litekanyetso tsa RIN <7, mokhoa oa Ribo-off (Vazyme #N406/407/408/409) o loketse ho tlosoa ha rRNA.
Lihlahisoa le lits'ebeletso tsena li fanoe molemong oa hau.News-Medical.Net ha e khothaletse leha e le efe ea lihlahisoa le litšebeletso tsena.
Nako ea poso: May-06-2022