Ungawenza Kanjani Amavolumu Amancane Nge-Handheld Manual Pipettes

Njengoba kugxilwa kakhulu ekwehliseni ama-reagents nezindleko, amanani amancane adingeka kakhulu, isb, ukulungiswa kwe-PCR Mastermix noma ukusabela kwe-enzyme.Kodwa ukufaka ngamapayipi amavolumu amancane ukusuka ku-0.2 – 5 µL kusetha izinselele ezintsha zokunemba nokunemba kwamapayipi.Amaphuzu alandelayo abalulekile:

1. I-Pipette nosayizi wethiphu: Ngaso sonke isikhathi khetha i-pipette enevolumu yezwi ephansi kakhulu ngangokunokwenzeka kanye nethiphu encane kakhulu ukuze ugcine umcamelo womoya umncane ngangokunokwenzeka.Uma ufaka ipayipi elingu-1 µL isb, khetha ipayipi elingu-0.25 – 2.5 µL kanye nethiphu elifanayo kune-pipette engu-1 – 10 µL.
2. Ukulinganisa nokugcinwa: Kubalulekile ukuthi amapayipi akho alinganiswe kahle futhi anakekelwe.Ukulungiswa okuncane kanye nezingxenye eziphukile ku-pipette kuholela ekwandeni okukhulu kwamanani amaphutha ahlelekile futhi angahleliwe.Ukulinganisa ngokwe-ISO 8655 kumele kwenziwe kanye ngonyaka.
3. Amapayipi ahamba kahle: Hlola ukuthi unayo yini i-pipette eguquguqukayo enebanga eliphansi levolumu elebhu yakho.Ngokuvamile, ukusebenzisa lolu hlobo lwe-pipette kuholela kumphumela ongcono we-pipettting mayelana nokunemba nokunemba kunama-pipettes ajwayelekile e-air-cushion.
4. Zama ukusebenzisa amavolumu amakhulu: Ungase ucabange ukuhlambulula isampula yakho kumavolumu amakhulu nge-pipette ngenani elifanayo ekuphenduleni kokugcina.Lokhu kunganciphisa amaphutha epayipi ngamavolumu amancane kakhulu wesampula.

Ngaphezu kwethuluzi elihle, umcwaningi kumele abe nenqubo yepayipi elihle kakhulu.Naka kakhulu izinyathelo ezilandelayo:

1. Okunamathiselwe kwithiphu: Ungagxumeli ipayipi ichopho njengoba lokhu kungase kulimaze ichopho elicolekile kubangele ukuthi ugongolo oluwuketshezi luqondiswe kabusha noma kulimaze i-orifice.Faka ingcindezi encane kuphela lapho unamathisela ithiphu futhi usebenzise i-pipette ene-spring-loaded tip cone.
2. Ukubamba i-pipette: Ungayibambi i-pipette esandleni sakho ngenkathi ulinde i-centrifuge, i-cycler, njll. Ingaphakathi le-pipette lizoshisa futhi liholele umcamelo womoya ukuze ande okuholela ekuphambukeni kuvolumu ebekiwe lapho ipayipi.
3. Ukumanzisa ngaphambili: Ukunyakaziswa komoya ngaphakathi kwethiphu ne-pipette kulungiselela ithiphu lesampula futhi kugwema ukuhwamuka lapho kulangazelela umthamo wokudlulisa.
4. Ukulangazelela okuqondile: Lokhu kubaluleke kakhulu lapho uphatha imiqulu emincane ukuze ugweme umphumela we-capillary okwenzeka lapho i-pipette ibanjwe nge-engeli.
5. Ukujula kokucwiliswa: Gxilisa ithiphu kancane ngangokunokwenzeka ukuze uvimbele uketshezi ukuthi lungene ichopho ngenxa yomphumela we-capillary.Umthetho wesithupha: Uma ithiphu nevolumu lincane, kuyancipha ukujula kokucwiliswa.Sincoma ubukhulu obungu-2 mm lapho ufaka amavolumu amancane.
6. Ukukhipha nge-engeli engu-45°: Ukugeleza okuphelele kokuphuma koketshezi kuqinisekiswa uma i-pipette ibanjwe nge-engeli engu-45°.
7. Ukuthintana nodonga lomkhumbi noma indawo ewuketshezi: Imiqulu emincane ingakhishwa kahle kuphela lapho ithiphu ibanjelwe odongeni lomkhumbi, noma icwiliswa oketshezini.Ngisho nokwehla kokugcina esiphundu kungakhishwa ngokunembile.
8. Ukukhipha umoya: Ukukhipha umoya kuyimpoqo ngemva kokukhipha amavolumu aphansi ukuze kukhishwe ngisho neconsi lokugcina loketshezi olukhona kwithiphu.Ukuqhuma kufanele futhi kwenziwe ngokumelene nodonga lomkhumbi.Qaphela ukuthi ungalethi amabhamuza omoya kusampula lapho ukhipha umoya endaweni ewuketshezi.