Uwenziwa njani uPipette amaVolumes amaNcinci ngePipettes yeManuwali ephathwa ngesandla

Njengoko kugxininiswa ngakumbi ekunciphiseni ii-reagents kunye neendleko, amanani amancinci afunwa kakhulu, umzekelo, ukulungiselela i-PCR Mastermix okanye i-enzyme reactions.Kodwa ukubhobhoza imibhobho emincinci ukusuka kwi-0.2 - 5 µL ibeka imingeni emitsha yokuchaneka kombhobho kunye nokuchaneka.Ezi ngongoma zilandelayo zibalulekile:

1. I-Pipette kunye nobungakanani bencam: Soloko ukhetha i-pipette enomthamo ophantsi onokwenzeka kunye nencam encinci yokugcina umqamelo womoya umncinci kangangoko.Xa ufaka i-1 µL umz., khetha i-0.25 – 2.5 µL ipipetti kunye nencam ehambelanayo kune-1 – 10 µL ipiipi.
2. Ukulinganisa kunye nokugcinwa: Kubalulekile ukuba iipayipi zakho zilinganiswe ngokufanelekileyo kwaye zigcinwe.Uhlengahlengiso oluncinci kunye neengxenye eziqhekekileyo kwi-pipette zikhokelela ekunyukeni okukhulu kwemilinganiselo yempazamo ecwangcisiweyo kunye ne-random.Ulungelelwaniso ngokwe-ISO 8655 kufuneka lwenziwe kube kanye ngonyaka.
3. Iipipette zokufuduka ezilungileyo: Khangela ukuba unepipette efanelekileyo yokufuduka enoluhlu oluphantsi lwevolumu kwilebhu yakho.Ngokuqhelekileyo, ukusebenzisa olu hlobo lwe-pipette lukhokelela kwisiphumo esingcono sepayipi ngokubhekiselele ngokuchanekileyo kunye nokuchaneka kune-classic air-cushion pipettes.
4. Zama ukusebenzisa imiqulu emikhulu: Unokucinga ngokuhlambulula isampuli yakho kwi-pipette imiqulu emikhulu kunye nobuninzi obufanayo kwimpendulo yokugqibela.Oku kunokunciphisa iimpazamo zemibhobho ngeesampulu ezincinci kakhulu.

Ukongeza kwisixhobo esilungileyo, umphandi kufuneka abe nobuchwephesha obuhle kakhulu bepayipi.Nika ingqwalasela eyodwa kula manyathelo alandelayo:

1. Ingcebiso yokuncamathisela: Musa ukujamza i-pipette kwincam njengoko oku kungonakalisa isiphelo sencam ecolekileyo kubangele ukuba umqadi wolwelo uqondiswe ngokutsha okanye wonakalise i-orifice.Faka kuphela uxinzelelo olulula xa udibanisa isicatshulwa kwaye usebenzise i-pipette ene-spring-loaded tip cone.
2. Ukubamba i-pipette: Musa ukubamba i-pipette esandleni sakho ngelixa ulinde i-centrifuge, i-cycler, njl.
3. Ukumanzisa kwangaphambili: Ukufuma komoya ngaphakathi kwincam kunye ne-pipette ilungiselela i-tip yesampuli kwaye igweme ukukhupha umoya xa unqwenela umthamo wokudlulisa.
4. Ukunqwenela ngokuthe nkqo: Oku kubaluleke kakhulu xa uphatha amanani amancinci ukuphepha umphumo we-capillary owenzeka xa i-pipette ibanjwe kwi-angle.
5. Ubunzulu bokuntywiliselwa: ntywilisela incam kancinane kangangoko ukuthintela ulwelo ukuba lungene kwincam ngenxa yesiphumo secapillary.Umgaqo wesithupha: Incinci incam kunye nomthamo, isezantsi ubunzulu bokuntywiliselwa.Sincoma ubuninzi be-2 mm xa ufaka imibhobho emincinci emincinci.
6. Ukukhupha kwi-angle ye-45 °: Ukuphuma okulungileyo kokuphuma kwamanzi kuqinisekisiwe xa i-pipette ibanjwe kwi-angle ye-45 °.
7. Uqhagamshelwano nodonga lwenqanawa okanye umphezulu wolwelo: Imithamo emincinci ingakhutshwa ngokufanelekileyo kuphela xa incam ibanjwe eludongeni lwenqanawa, okanye intywiliselwe kulwelo.Nokuba ithontsi lokugqibela elivela kwincam lingasasazwa ngokuchanekileyo.
8. Ukuvuthela: Ukuvuthela kunyanzelekile emva kokukhupha imithamo ephantsi ukukhupha nethontsi lokugqibela lolwelo elikhoyo kwincam.Ukuvuthela kufuneka kuqhutywe ngokuchasene nodonga lwenqanawa.Kulumkele ukuzisa amaqamza omoya kwisampulu xa uvuthela kumphezulu wolwelo.