How to use PCR plate to do experiment?

PCR (polymerase chain reaction) plates are used to conduct PCR experiments, which are widely used in molecular biology research to amplify DNA sequences.

Here are the general steps to use a PCR plate for a typical experiment:

  1. Prepare your PCR reaction mix: Prepare your PCR reaction mix according to the protocol of your experiment, which typically includes a template DNA, PCR primers, dNTPs, Taq polymerase, buffer and other additives.
  2. Add the reaction mix to the PCR plate: Using a multi-channel pipette or a manual pipette, add the reaction mix to the wells of the PCR plate. Be sure to avoid introducing air bubbles into the reaction mix, as this can affect the results of your experiment.
  3. Add your template DNA to the reaction mix: Depending on your experiment, you may need to add your template DNA to the reaction mix. If you’re using a multi-channel pipette, be sure to change the tips between samples to avoid cross-contamination.
  4. Seal the plate: Once you’ve added the reaction mix and template DNA to the PCR plate, seal the plate with a suitable seal, such as a PCR plate sealing film or cap strip.
  5. Place the plate in the thermocycler: Finally, place the sealed PCR plate in the thermocycler and run your PCR program, which typically consists of a series of temperature cycles that allow the DNA to be amplified.

After the PCR reaction is complete, you can analyze the products using a variety of techniques, such as gel electrophoresis or sequencing. Be sure to follow the specific protocols of your experiment to obtain accurate and reliable results.

 

Suzhou Ace Biomedical is a leading manufacturer of high-quality PCR consumables. We are dedicated to providing reliable and efficient tools for your PCR experiments, with a range of products designed to meet the needs of researchers in a variety of fields.

Our PCR consumables include PCR plates, PCR tubes, PCR tube strips, and sealing films. All of our products are made with high-quality materials, ensuring that they can withstand the rigors of the PCR process and produce consistent and accurate results.

At Suzhou Ace Biomedical, we understand the importance of precision in your PCR experiments. That’s why our PCR consumables are manufactured with the highest standards of quality and undergo rigorous testing to ensure that they meet or exceed your expectations. Our products are also designed to be compatible with a wide range of thermocyclers, making them a versatile choice for researchers in different labs.

Whether you are conducting basic research, clinical diagnostics, or other applications, Suzhou Ace Biomedical has the PCR consumables you need to achieve your goals. We are committed to providing exceptional products and customer service, and we are proud to be a trusted partner for researchers around the world.

Contact us today to learn more about our PCR consumables and how we can support your research.


Post time: Feb-15-2023