Ungalisebenzisa kanjani ipuleti le-PCR ukwenza isilingo?

Amapuleti e-PCR (polymerase chain reaction) asetshenziselwa ukwenza izivivinyo ze-PCR, ezisetshenziswa kakhulu ocwaningweni lwebhayoloji yamangqamuzana ukuze kukhuliswe ukulandelana kwe-DNA.

Nazi izinyathelo ezijwayelekile zokusebenzisa aIpuleti le-PCRngokuhlolwa okujwayelekile:

1. Lungiselela imiksi yakho yokusabela kwe-PCR: Lungiselela imiksi yakho yokusabela kwe-PCR ngokuya ngephrothokholi yokuhlolwa kwakho, ngokuvamile ehlanganisa i-DNA yesifanekiso, iziqalo ze-PCR, ama-dNTP, i-Taq polymerase, ibhafa nezinye izithasiselo.
2. Engeza imiksi yokusabela epuletini le-PCR: Usebenzisa i-pipette eneziteshi eziningi noma i-pipette eyenziwa ngesandla, engeza ingxube yokusabela emithonjeni yepuleti le-PCR.Qiniseka ukuthi ugwema ukwethula amabhamuza omoya emiksini yokusabela, njengoba lokhu kungase kuthinte imiphumela yokuhlolwa kwakho.
3. Engeza i-DNA yesifanekiso sakho ekuxubeni kokusabela: Kuye ngokuhlolwa kwakho, kungase kudingeke wengeze i-DNA yesifanekiso sakho kungxube yokusabela.Uma usebenzisa i-pipette enamashaneli amaningi, qiniseka ukuthi ushintsha amathiphu phakathi kwamasampula ukuze ugweme ukungcoliseka.
4. Vala ipuleti: Uma usungeze ingxube yokusabela nesifanekiso se-DNA epuletini le-PCR, vala ipuleti ngophawu olufanele, njengefilimu evala ipuleti le-PCR noma i-cap strip.
5. Beka ipuleti ku-thermocycler: Okokugcina, beka ipuleti le-PCR elivaliwe ku-thermocycler bese uqhuba uhlelo lwakho lwe-PCR, ngokuvamile oluhlanganisa uchungechunge lwemijikelezo yezinga lokushisa evumela ukuthi i-DNA ithuthukiswe.

Ngemuva kokuthi ukusabela kwe-PCR sekuqediwe, ungahlaziya imikhiqizo usebenzisa amasu ahlukahlukene, njengejeli electrophoresis noma ukulandelana.Qiniseka ukuthi ulandela amaphrothokholi athile okuhlolwa kwakho ukuze uthole imiphumela enembile nethembekile.

I-Suzhou Ace Biomedicalingumkhiqizi ohamba phambili wekhwalithi ephezuluIzinto ezisetshenziswayo ze-PCR.Sizinikele ekukunikezeni amathuluzi athembekile nasebenza kahle okuhlolwa kwakho kwe-PCR, nezinhlobonhlobo zemikhiqizo eklanyelwe ukuhlangabezana nezidingo zabacwaningi emikhakheni eyahlukene.

I-PCR yethu ihlanganisaAmapuleti e-PCR, amashubhu e-PCR, imichilo yeshubhu ye-PCR, namafilimu okuvala uphawu.Yonke imikhiqizo yethu yenziwe ngezinto ezisezingeni eliphezulu, iqinisekisa ukuthi ingakwazi ukumelana nobunzima benqubo ye-PCR futhi ikhiqize imiphumela engaguquki nenembile.

Kwa-Suzhou Ace Biomedical, siyakuqonda ukubaluleka kokunemba ekuhlolweni kwakho kwe-PCR.Kungakho izinto zethu ezisebenzisekayo ze-PCR zakhiwe ngezinga eliphezulu kakhulu lekhwalithi futhi zihlolwa ngokuqinile ukuze kuqinisekiswe ukuthi ziyahlangabezana noma zidlule okulindele.Imikhiqizo yethu futhi yakhelwe ukuthi ihambisane nohlu olubanzi lwama-thermocyclers, okuyenza ibe yinketho eguquguqukayo yabacwaningi kumalebhu ahlukene.

Noma ngabe wenza ucwaningo oluyisisekelo, ukuxilonga imitholampilo, noma ezinye izinhlelo zokusebenza, i-Suzhou Ace Biomedical inezinto ezisetshenziswayo ze-PCR ozidingayo ukuze ufeze izinjongo zakho.Sizibophezele ekuhlinzekeni ngemikhiqizo eyingqayizivele kanye nesevisi yamakhasimende, futhi siyaziqhenya ngokuba uzakwethu othembekile wabacwaningi emhlabeni wonke.

Xhumana nathi namuhla ukuze ufunde kabanzi mayelana nezinto ezisetshenziswayo ze-PCR nokuthi singalusekela kanjani ucwaningo lwakho.