Momwe mungagwiritsire ntchito mbale ya PCR kuyesa?

Mambale a PCR (polymerase chain reaction) amagwiritsidwa ntchito poyesa kuyesa kwa PCR, komwe kumagwiritsidwa ntchito kwambiri pakufufuza kwa ma molekyulu a biology kukulitsa kutsatizana kwa DNA.

Nazi njira zambiri zogwiritsira ntchito aChithunzi cha PCRzoyeserera wamba:

1. Konzani kaphatikizidwe ka PCR yanu: Konzani zosakaniza zanu za PCR molingana ndi ndondomeko ya kuyesa kwanu, komwe kumaphatikizapo template ya DNA, PCR primers, dNTPs, Taq polymerase, buffer ndi zina zowonjezera.
2. Onjezani zomwe zimasakanikirana ndi mbale ya PCR: Pogwiritsa ntchito pipette yamitundu yambiri kapena pipette yamanja, onjezerani kusakaniza ku zitsime za mbale ya PCR.Onetsetsani kuti mupewe kuyambitsa thovu la mpweya muzosakaniza, chifukwa izi zingakhudze zotsatira za kuyesa kwanu.
3. Onjezani template yanu ya DNA pazosakanikirana: Kutengera zomwe mwayesa, mungafunike kuwonjezera template yanu ya DNA pazosakanikirana.Ngati mukugwiritsa ntchito pipette yamitundu yambiri, onetsetsani kuti mwasintha nsonga pakati pa zitsanzo kuti mupewe kuipitsidwa.
4. Sindikiza mbaleyo: Mukangowonjezerapo kusakaniza ndi template ya DNA ku mbale ya PCR, sindikizani mbaleyo ndi chidindo choyenera, monga filimu yosindikiza ya PCR kapena kapu.
5. Ikani mbaleyo mu thermocycler: Pomaliza, ikani mbale ya PCR yosindikizidwa mu thermocycler ndikuyendetsa pulogalamu yanu ya PCR, yomwe imakhala ndi maulendo angapo a kutentha omwe amalola kuti DNA ikulitsidwe.

PCR ikatha, mutha kusanthula zinthuzo pogwiritsa ntchito njira zosiyanasiyana, monga gel electrophoresis kapena kutsatizana.Onetsetsani kuti mukutsatira ndondomeko za kuyesa kwanu kuti mupeze zotsatira zolondola komanso zodalirika.

Suzhou Ace Biomedicalndi wopanga wapamwamba kwambiriZithunzi za PCR.Ndife odzipereka kuti tikupatseni zida zodalirika komanso zogwira mtima pazoyeserera zanu za PCR, zokhala ndi zinthu zingapo zomwe zimapangidwa kuti zikwaniritse zosowa za ofufuza m'magawo osiyanasiyana.

Zogulitsa zathu za PCR zikuphatikizaMa mbale a PCR, machubu a PCR, machubu a PCR, ndi mafilimu osindikiza.Zogulitsa zathu zonse zimapangidwa ndi zipangizo zamtengo wapatali, kuonetsetsa kuti zimatha kupirira zovuta za ndondomeko ya PCR ndikupanga zotsatira zogwirizana komanso zolondola.

Ku Suzhou Ace Biomedical, timamvetsetsa kufunikira kolondola pakuyesa kwanu kwa PCR.Ichi ndichifukwa chake zida zathu za PCR zimapangidwa ndi miyezo yapamwamba kwambiri ndipo zimayesedwa mwamphamvu kuti zitsimikizire kuti zikukwaniritsa kapena kupitilira zomwe mukuyembekezera.Zogulitsa zathu zidapangidwanso kuti zizigwirizana ndi mitundu ingapo ya ma thermocyclers, kuwapanga kukhala chisankho chosunthika kwa ofufuza m'ma lab osiyanasiyana.

Kaya mukuchita kafukufuku wofunikira, zowunikira zamankhwala, kapena ntchito zina, Suzhou Ace Biomedical ili ndi zinthu za PCR zomwe mungafune kuti mukwaniritse zolinga zanu.Ndife odzipereka kupereka zinthu zapadera komanso ntchito zamakasitomala, ndipo ndife onyadira kukhala bwenzi lodalirika la ofufuza padziko lonse lapansi.

Lumikizanani nafe lero kuti mudziwe zambiri za PCR yathu yogwiritsira ntchito komanso momwe tingathandizire kafukufuku wanu.