Indlela ye-revolutionary polymerase chain reaction (PCR) ibe negalelo elikhulu ekuthuthukisweni kolwazi lomuntu emikhakheni eminingi yocwaningo, ukuxilonga kanye ne-forensics.Izimiso ze-PCR ezijwayelekile zibandakanya ukukhuliswa kokulandelana kwe-DNA kokuthakaselayo kusampula, futhi ngemva kokuqedwa kokusabela, ukuba khona noma ukungabikho kwalokhu kulandelana kwe-DNA kunqunywa ekupheleni kwephuzu lokuhlaziya.Ngesikhathi sobhadane lwe-Covid-19, i-PCR yesikhathi sangempela ekala ukunqwabelana kwemikhiqizo yokukhulisa izingane njengoba ukusabela kuthuthuka, ihlinzeka ngenani ngemva komjikelezo ngamunye, isiphenduke indlela esezingeni eligolide yokuhlola iziguli ukuthi zihlonzwe yini i-SARS-COV-2.
I-Real-time PCR, eyaziwa nangokuthi i-quantitative PCR (qPCR), isebenzisa izinhlobonhlobo zamakhemikhali e-fluorescent ahlobanisa ukugxila komkhiqizo we-PCR namandla e-fluorescence.Ngemva komjikelezo ngamunye we-PCR, i-fluorescence iyalinganiswa futhi ubukhulu besiginali ye-fluorescence bubonisa inani lama-amplicon e-DNA kusampula ngaleso sikhathi esicacisiwe.Lokhu kukhiqiza ijika le-qPCR, lapho ukuqina kwesignali echaziwe kufanele kudlulwe kuze kube kunomkhiqizo owanele wokuthi i-fluorescence ibonakale ngemuva.Ijika lisetshenziselwa ukunquma inani le-DNA eqondiwe.
Ngokuhamba kwesikhathi, amalabhorethri asebenzise ukusetshenziswa kwamapuleti anemithombo eminingi ukucubungula amasampula amaningi ngesikhathi esisodwa, okuvumela ukuphuma okuphezulu.Kodwa-ke, amasampula adinga ukuvikelwa ekungcoleni nasekuhwameni ukuze kuqinisekiswe izinga eliphezulu lemiphumela.Indlela ye-PCR izwela kakhulu ekungcolisweni yi-DNA yangaphandle, ngakho-ke kubaluleke kakhulu ukugcina indawo ihlanzekile.Ukucaca okuphezulu kwe-optical kanye nokuphazamiseka okuncane nakho kubalulekile ukuze kuqinisekiswe ukufundwa okunembile kwesiginali ye-fluorescent.I-PCR plate seal iyatholakala ukwenza lo msebenzi futhi kunezinhlobo ezahlukene zezimpawu ezitholakalayo zamasampula ahlukahlukene, izinqubo zokuhlola kanye nezinto ezithandwa nguwe.Uma kuqhathaniswa nezinye izindlela zokuvala, ukusetshenziswa kwe-adhesive plate sealing kulula kakhulu futhi kuyabiza.
Ukuvala amafilimu kusukaI-Suzhou Ace Biomedicalibe nokucaca okuphezulu kwe-optical okunamathelayo kwebanga lezokwelapha okungabambisi, okungashisi ukukhanya, okufanelekile ezinhlelweni ze-PCR zesikhathi sangempela.Lezi zakhiwo zibalulekile ukuze kuqinisekiswe ukuthi amafilimu okubeka uphawu awabangeli ukuphazamiseka emiphumeleni etholiwe.
Amafilimu okuvalwa aphinde aqinisekiswe i-DNase, i-RNase ne-nucleic acid mahhala ukuze abasebenzisi baqiniseke ukuthi akukho ukungcoliswa kwamasampuli futhi imiphumela inembile.
Yiziphi Izinzuzo Zezimpawu Ezinamathelayo?
Izigxivizo ezinamathelayo ziyashesha futhi kulula ukuzisebenzisa ngokusebenza okuqondile phezu kwamapuleti ekuhambeni komsebenzi okwenziwa ngesandla ukuze kuvikelwe okuqukethwe kwamapuleti okwesikhashana.Futhi ukucaca okungaguquki kwe-ultra-high optical kwenza izilinganiso zokukhulisa i-DNA ezikwazi ukukhiqizwa kabusha, ezinokwethenjelwa nezinembile.
Ingcina eqinile, eqinile, ekwazi ukumelana nokushisa iqinisekisa ukuvaleka okuthembekile eduze komthombo ngamunye.Futhi afaka amathebhu aneziphetho ezimbili asiza ekubekeni ifilimu yokuvala futhi angasuswa ukuze kuvinjelwe ukukhushulwa kanye namazinga aphezulu okuhwamuka.
Ukuvala amafilimu kunciphisa ukuhwamuka, kunciphisa ukungcoliswa kwesiphambano futhi kuvimbele ukuchitheka - okubaluleke kakhulu lapho kusebenza amasampula aqukethe ama-molecule egciwane kanye namagciwane abeka engcupheni kumuntu.
Uhlu olubanzi lwamanye amapuleti seal ayatholakala kuwoI-Suzhou Ace Biomedicalngezakhiwo ezithile eziklanyelwe izinhlelo zokusebenza ezifana ne-PCR evamile, isitoreji sesikhathi esifushane kanye nesikhathi eside.
Isikhathi sokuthumela: Sep-15-2022