Akamaro ka PCR ifunga plaque

Tekinike ya polymerase yimpinduramatwara (PCR) yagize uruhare runini mugutezimbere mubumenyi bwabantu mubice byinshi byubushakashatsi, kwisuzumisha nubucamanza.Amahame ya PCR asanzwe arimo kwongerera ADN urutonde rwinyungu zicyitegererezo, kandi nyuma yo kurangiza reaction, kuba ADN ikurikiranye cyangwa idahari bigenwa nisesengura ryanyuma.Mugihe cyicyorezo cya Covid-19, igihe nyacyo PCR gipima ikusanyirizo ryibicuruzwa byongerewe imbaraga uko reaction igenda itera, gutanga umubare nyuma ya buri cyiciro, byahindutse uburyo bwa zahabu bwo gupima abarwayi kugirango basuzume SARS-COV-2.

Igihe nyacyo PCR, kizwi kandi ku bwinshi bwa PCR (qPCR), ikoresha imiti itandukanye ya chemisties ya fluorescent ihuza ibicuruzwa bya PCR nuburemere bwa fluorescence.Nyuma ya buri cyiciro cya PCR, hapimwe fluorescence kandi ubukana bwikimenyetso cya fluorescence bugaragaza ubwinshi bwa amplicons ya ADN murugero muricyo gihe cyihariye.Ibi bibyara umurongo wa qPCR, aho ibimenyetso byasobanuwe bigomba kurenga kugeza igihe habaye ibicuruzwa bihagije kugirango fluorescence iboneke inyuma.Umurongo ukoreshwa kugirango umenye ingano ya ADN igenewe.

Igihe kirenze, laboratoire zashyize mubikorwa ikoreshwa ryamasahani menshi kugirango itunganyirize ingero nyinshi icyarimwe, zitanga ibicuruzwa byinshi.Nyamara, ibyitegererezo bigomba kurindwa kwanduzwa no guhumeka kugirango harebwe ibisubizo byiza.Tekinike ya PCR yunvikana cyane kwanduzwa na ADN idasanzwe, bityo rero ni ngombwa cyane kubungabunga ibidukikije bisukuye.Umubare ntarengwa wa optique no kwivanga ntarengwa nabyo ni ngombwa kugirango umenye neza ibimenyetso bya fluorescent.Ikimenyetso cya PCR kirahari kugirango ukore iki gikorwa kandi hari ubwoko butandukanye bwa kashe iboneka kuburugero rutandukanye, inzira zigeragezwa hamwe nibyo ukunda.Ugereranije nubundi buryo bwo gufunga, gukoresha kashe ya plaque bifunze biroroshye kandi birahendutse.

Gufunga firime kuvaSuzhou Ace Biomedicalzifite optique isobanutse neza idakwega, idafite fluorescing yo murwego rwo kwa muganga, ikwiranye nigihe gikwiye cya PCR.Iyi mitungo ningirakamaro kugirango tumenye neza ko firime zifunga zidatera intambamyi kubisubizo byabonetse.

Firime zifunga kandi zemewe DNase, RNase na acide nucleic aside kuburyo abakoresha bashobora kumenya neza ko nta kwanduza ingero nibisubizo ari ukuri.

Ni izihe nyungu zo gufunga kashe?
Ikidodo gifatika kirihuta kandi cyoroshye gukoresha hamwe na progaramu itaziguye hejuru yamasahani mumurimo wintoki kugirango urinde ibiri mumasahani by'agateganyo.Kandi ultra-high optique isobanutse ituma ibipimo byororoka byizewe, byizewe kandi byukuri.

Inert, ikomeye, irwanya ubushyuhe yizeza ikidodo cyizewe kuri buri riba.Bagaragaza kandi tab-end ebyiri zifasha mugushira firime ya kashe kandi irashobora gukurwaho kugirango wirinde guterura nigipimo cyinshi cyo guhumeka.

Gufunga firime bigabanya guhumeka, kugabanya kwanduzanya no gukumira isuka - ibyo ni ingenzi cyane mugihe ukoresheje ingero zirimo molekile za virusi na bagiteri zitera umuntu ingaruka.

Ubwoko bunini bwibindi bimenyetso birahari kuvaSuzhou Ace Biomedicalhamwe nibintu byihariye byagenewe porogaramu nka PCR isanzwe, kubika igihe gito no kubika igihe kirekire.