Kufunika kwa PCR kusindikiza mbale filimu

Njira ya revolutionary polymerase chain reaction (PCR) yathandizira kwambiri kupititsa patsogolo chidziwitso cha anthu m'magawo angapo a kafukufuku, zowunikira komanso zazamalamulo.Mfundo za PCR wamba zimaphatikizira kukulitsa kutsatizana kwa DNA pachitsanzo, ndipo zikamaliza zomwe zimachitika, kupezeka kapena kusapezeka kwa mndandanda wa DNAwu kumatsimikiziridwa pakuwunika komaliza.Munthawi ya mliri wa Covid-19, PCR yeniyeni yomwe imayesa kuchuluka kwa zinthu zokulitsa momwe momwe zimakhalira, kupereka kuchulukana pambuyo pa kuzungulira kulikonse, yakhala njira yagolide yoyesera odwala kuti adziwe kuti ali ndi SARS-COV-2.

Real-time PCR, yomwe imadziwikanso kuti quantitative PCR (qPCR), imagwiritsa ntchito mitundu ingapo yamafakitale a fulorosenti omwe amalumikizana ndi kuchuluka kwa zinthu za PCR ndi kuchuluka kwa fluorescence.Pambuyo pa kuzungulira kulikonse kwa PCR, fluorescence imayesedwa ndipo mphamvu ya chizindikiro cha fluorescence imasonyeza kuchuluka kwa ma amplicon a DNA mu zitsanzo panthawiyo.Izi zimapanga qPCR yokhotakhota, momwe mphamvu ya siginecha yodziwika iyenera kupitilira mpaka patakhala chinthu chokwanira kuti fulorosenti iwonekere chakumbuyo.Kholo limagwiritsidwa ntchito kudziwa kuchuluka kwa DNA yomwe mukufuna.

M'kupita kwa nthawi, ma laboratories akhazikitsa kugwiritsa ntchito mbale zokhala ndi zitsime zambiri kuti azikonza zitsanzo zambiri nthawi imodzi, zomwe zimapangitsa kuti pakhale kutulutsa kwakukulu.Komabe, zitsanzozo ziyenera kutetezedwa kuti zisaipitsidwe ndi kutuluka kwa nthunzi kuti zitsimikizire zotsatira zapamwamba.Njira ya PCR imakhudzidwa kwambiri ndi kuipitsidwa ndi DNA yakunja, motero ndikofunikira kwambiri kukhala ndi malo aukhondo.Kuwoneka bwino kwambiri komanso kusokoneza pang'ono ndikofunikira kuti mutsimikizire kuwerengera kolondola kwa siginecha ya fulorosenti.Zisindikizo za PCR mbale zilipo kuti zigwire ntchitoyi ndipo pali mitundu yosiyanasiyana ya zisindikizo zomwe zilipo pazitsanzo zosiyanasiyana, njira zoyesera ndi zokonda zaumwini.Poyerekeza ndi njira zina zosindikizira, kugwiritsa ntchito kusindikiza mbale zomatira ndikosavuta komanso kotsika mtengo.

Kusindikiza mafilimu kuchokeraSuzhou Ace Biomedicalkukhala ndi kuwala kwapamwamba kokhala ndi zomatira zachipatala zosayamwa, zopanda fluorescing, zoyenera kugwiritsa ntchito PCR zenizeni.Zinthuzi ndizofunikira kuti zitsimikizire kuti mafilimu osindikizira samayambitsa kusokoneza kulikonse kwa zotsatira zomwe zapezedwa.

Mafilimu osindikizira alinso ndi DNase, RNase ndi nucleic acid yaulere kotero kuti ogwiritsa ntchito atsimikizire kuti palibe kuipitsidwa kwa zitsanzo ndipo zotsatira zake ndi zolondola.

Kodi Ubwino Wa Zomatira Zisindikizo Ndi Chiyani?
Zisindikizo zomatira ndizofulumira komanso zosavuta kugwiritsa ntchito ndikugwiritsa ntchito mwachindunji pama mbale pamakina ogwirira ntchito kuti muteteze zomwe zili m'mbale kwakanthawi.Ndipo kumveka kosasinthika kwapamwamba kwambiri kumapangitsa kuti miyeso yowonjezereka ya DNA ikhale yochulukirachulukira, yodalirika komanso yolondola.

Zomatira zoziziritsa kukhosi, zolimba, zosagwira kutentha zimatsimikizira kusindikiza kodalirika pachitsime chilichonse.Amakhalanso ndi ma tabo okhala ndi mapeto awiri omwe amathandizira kuyika filimu yosindikizira ndipo amatha kuchotsedwa kuti ateteze kukweza ndi kuwonjezereka kwa evaporation.

Makanema osindikiza amachepetsa kutuluka kwa nthunzi, amachepetsa kuipitsidwa ndikuletsa kutayikira - zomwe ndizofunikira kwambiri pochita ndi zitsanzo zomwe zili ndi ma virus ndi mabakiteriya omwe ali pachiwopsezo kwa munthu.

Mitundu yambiri ya zisindikizo za mbale zimapezeka kuchokeraSuzhou Ace Biomedicalyokhala ndi zida zapadera zopangidwira ntchito monga PCR yokhazikika, kusungirako kwakanthawi kochepa komanso kwanthawi yayitali.