Inkqubo yerevolutionary polymerase chain reaction (PCR) yenze igalelo elibalulekileyo ekuqhubeleni phambili kolwazi lwabantu kwiindawo ezininzi zophando, uxilongo kunye ne forensics.Imigaqo ye-PCR eqhelekileyo ibandakanya ukukhulisa ulandelelwano lwe-DNA lomdla kwisampulu, kwaye emva kokugqitywa kwempendulo, ubukho okanye ukungabikho kolu landelelwano lwe-DNA kugqitywa ekupheleni kohlalutyo.Ngexesha lobhubhani we-Covid-19, i-PCR yexesha lokwenyani elinganisa ukuqokelelwa kweemveliso zokukhulisa njengoko kuqhubeka ukusabela, ukubonelela ngobungakanani emva komjikelo ngamnye, iye yaba yindlela esemgangathweni yegolide yokuvavanya izigulana zofunyaniso lwe-SARS-COV-2.
I-PCR yexesha lokwenyani, ekwabizwa ngokuba yi-PCR yobungakanani (qPCR), isebenzisa iintlobo ngeentlobo zeekhemistri zefluorescent ezinxibelelanisa ingxinano yemveliso ye-PCR kubunzulu be-fluorescence.Emva komjikelo ngamnye we-PCR, i-fluorescence iyalinganiswa kwaye ubunzulu bomqondiso we-fluorescence bubonisa ubuninzi be-DNA amplicon kwisampulu ngelo xesha.Oku kuvelisa ijiko le-qPCR, apho ubunzulu bophawu oluchaziweyo kufuneka kugqithwe kude kubekho imveliso eyaneleyo ye-fluorescence ukuze ibonwe ngasemva.Igophe lisetyenziselwa ukumisela ubuninzi be-DNA ekujoliswe kuyo.
Ngokuhamba kwexesha, iilabhoratri ziphumeze ukusetyenziswa kweepleyiti ezinamaqula amaninzi ukucubungula iisampuli ezininzi ngaxeshanye, zivumela ukuphuma okuphezulu.Nangona kunjalo, iisampulu kufuneka zikhuselwe ekungcoliseni kunye nokuguquka kwamanzi ukuze kuqinisekiswe umgangatho ophezulu weziphumo.Ubuchwephesha be-PCR bunovelwano kakhulu ekosulelweni yi-DNA yangaphandle, ngoko ke kubaluleke kakhulu ukugcina indawo engqongileyo ecocekileyo.Ukucaca okuphezulu kwe-optical kunye nokuphazamiseka okuncinci kubalulekile ukuqinisekisa ukufundwa ngokuchanekileyo kwesignali ye-fluorescent.PCR plate seals ziyafumaneka ukwenza lo msebenzi kwaye kukho iintlobo ezahlukeneyo zetywina ezikhoyo kwiisampuli ezahlukeneyo, iinkqubo zovavanyo kunye nezinto ozikhethayo.Xa kuthelekiswa nezinye iindlela zokutywina, ukusetyenziswa kwe-adhesive plate sealing kulula ngakumbi kwaye kuyabiza.
Ukutywina iifilimu ukusukaSuzhou Ace Biomedicalube nokucaca okuphezulu kwe-optical kunye ne-non-absorbing, i-non-fluorescing grade adhesive grade adhesive, efanelekileyo kwizicelo ze-PCR zexesha langempela.Ezi zakhiwo zibalulekile ukuqinisekisa ukuba iifilimu zokutywina azibangeli ukuphazamiseka kwiziphumo ezifunyenweyo.
Iifilimu zokutywinwa nazo ziqinisekisiwe i-DNase, i-RNase kunye ne-nucleic acid mahhala ukuze abasebenzisi baqiniseke ukuba akukho kungcola kweesampuli kunye neziphumo zichanekile.
Ziziphi iiNzuzo zeZitywina ezincamathelayo?
Iimpawu zokuncamathelisa zikhawuleza kwaye kulula ukuzisebenzisa kunye nesicelo esithe ngqo phezu kweepleyiti kwi-workflows manual ukukhusela imixholo yamacwecwe okwethutyana.Kwaye ukucaca okucacileyo kwe-ultra-high optical kwenza ukuveliswa kwakhona, ukuthembeka kunye nemilinganiselo yokukhulisa i-DNA echanekileyo.
Into encamathelayo engasebenziyo, eyomeleleyo, ekwaziyo ukumelana nobushushu iqinisekisa ukutywinwa okuthembekileyo kwiqula ngalinye.Zikwabonisa iithebhu zesiphelo ezibini ezinceda ekubekeni ifilimu yokutywina kwaye inokususwa ukunqanda ukunyuswa kunye namazinga aphezulu omoya.
Ukutywina iifilimu kunciphisa ukuvela komphunga, ukunciphisa ukungcoliseka kunye nokuthintela ukuchitheka - okubaluleke kakhulu xa ujongana neesampulu eziqulethe iimolekyuli zentsholongwane kunye neebhaktiriya ezibeka umngcipheko kumntu.
Uluhlu olubanzi lwezinye iipleyiti zokutywinwa ziyafumanekaSuzhou Ace Biomedicalngeepropati ezithile ezenzelwe izicelo ezifana ne-PCR eqhelekileyo, ixesha elifutshane kunye nokugcinwa kwexesha elide.
Ixesha lokuposa: Sep-15-2022