Njengompheki osebenzisa ummese, usosayensi udinga amakhono okushaya amapayipi.Umpheki ongumakad' ebona angakwazi ukusika isanqante sibe amaribhoni, abonakale engenamcabango, kodwa akulimazi neze ukugcina iziqondiso ezithile zepayipi engqondweni—kungakhathaliseki ukuthi usosayensi unolwazi olungakanani.Lapha, ochwepheshe abathathu banikeza amathiphu abo aphezulu.
“Umuntu kufanele aqikelele ukuthi abe nendlela efanele lapho ekhipha uketshezi mathupha,” kusho uMagali Gaillard, umphathi omkhulu, abaphathi bephothifoliyo, i-MLH Business Line, Gilson (Villiers-le-bel, France)."Amanye amaphutha ajwayelekile epayipi ahlobene nokusetshenziswa budedengu kwamathiphu e-pipette, isigqi esingahambisani noma isikhathi, kanye nokuphathwa okungafanele kwe-pipette."
Ngezinye izikhathi, usosayensi ngisho nokukhetha i-pipette engalungile.NjengoRishi Porecha, umphathi wemikhiqizo yomhlaba wonke kuImvulaI-Instruments (Oakland, CA), ithi, “Amanye amaphutha avamile ekufakweni kwamapayipi ahlanganisa ukungasebenzisi i-pipette yevolumu efanele emsebenzini othile kanye nokusebenzisa ipayipi elihambisa umoya ukuze libambe uketshezi olungenamanzi.”Ngamanzi e-viscous, i-piette-displacement pipette kufanele ihlale isetshenziswa.
Ngaphambi kokufika ezinkambisweni ezithile zamapayipi, eminye imiqondo ejwayelekile kufanele icatshangelwe.“Ngaso sonke isikhathi lapho abasebenzisi be-pipette beqala umsebenzi wosuku, kufanele bacabangele ukuthi yiluphi ucwaningo abalwenzayo, yiziphi izinto eziwuketshezi abasebenza ngazo, nokuthi yiziphi izinto ezisetshenziswayo abazifisayo ngaphambi kokukhetha ipayipi,” kusho uPorecha."Eqinisweni, ayikho ilebhu enawo wonke ama-pipettes umsebenzisi angawafisa, kodwa uma umsebenzisi ebheka ukuthi yimaphi amathuluzi atholakala elebhu kanye nomnyango, bangathola umbono ongcono wokuthi yiziphi ama-pipettes akhona okufanele asetshenziswe ekuhlolweni noma kwe-assay. imaphi ama-pipettes abangafuna ukuwathenga."
Izici ezitholakala kuma-pipettes anamuhla zidlulela ngale kwedivayisi ngokwayo.Ukuthuthuka ekuphatheni uketshezi kwenze kwaba nokwenzeka kubasebenzisi manje ukuxhuma i-pipette yabo efwini.Ngalokhu kuxhumana, umsebenzisi angalanda amaphrothokholi noma akhe angokwezifiso.Idatha yokufaka amapayipi ingathathwa ngisho nasefwini, okuyindlela eyodwa yokuhlonza noma yiziphi izinyathelo ezingalungile nokuthuthukisa inqubo yokufaka amapayipi, ikakhulukazi ngokulandelela ukunemba okuqhubekayo, noma ukuntuleka kwayo.
Njengoba unesisetshenziswa esifanele esandleni, inselele elandelayo ukulungisa izinyathelo.
Ukhiye Wempumelelo
Ngepayipi elisusa umoya, lezi zinyathelo ezilandelayo zandisa amathuba okulinganisa ngokunembile nangokuphindaphindiwe ivolumu ethile:
- Setha ivolumu ku-pipette.
- Cindezela i-plunger.
- Gxilisa ithiphu ekujuleni okulungile, okungahluka nge-pipette kanye nethiphu, futhi ngokushelelayo uvumele i-plunger iye endaweni yayo yokuphumula.
- Linda cishe umzuzwana owodwa ukuze uketshezi lugeleze lungeneithiphu.
- Beka i-pipette-ebanjwe ngama-degree angu-10-45-ngodonga lwekamelo elitholayo, futhi ucindezele kahle i-plunger esitobhini sokuqala.
- Linda umzuzwana owodwa bese ucindezela i-plunger uye esitobhini sesibili.
- Shelelisela ithiphu phezulu odongeni lomkhumbi ukuze ususe i-pipette.
- Vumela i-plunger ukuthi ibuyele endaweni yayo yokuphumula.
Isikhathi sokuthumela: Dec-12-2022