Njengompheki osebenzisa imela, isazinzulu sifuna ubuchule bokusebenzisa imibhobho.Umpheki onamava unokukwazi ukusika i-carrot kwiirebhoni, ebonakala engenangcinga, kodwa akukhe kube buhlungu ukugcina izikhokelo ezithile zemibhobho engqondweni-kungakhathaliseki ukuba isazinzulu sinamava kangakanani.Apha, iingcali ezintathu zinika iingcebiso zabo eziphezulu.
"Umntu kufuneka alumke ukuba abe nobuchule obufanelekileyo xa ehambisa ulwelo ngesandla," utshilo uMagali Gaillard, umphathi ophezulu, ulawulo lwepotfoliyo, iMLH Business Line, Gilson (Villiers-le-bel, France)."Ezinye zeempazamo eziqhelekileyo zepayipi zihambelana nokusetyenziswa ngokungakhathali kweengcebiso ze-pipette, isigqi esingahambelaniyo okanye ixesha, kunye nokuphathwa kakubi kwe-pipette."
Ngamanye amaxesha, inzululwazi ide ikhethe i-pipette engalunganga.NjengoRishi Porecha, umphathi wemveliso yehlabathi kwiIyanaIzixhobo (i-Oakland, CA), ithi, "Ezinye iimpazamo eziqhelekileyo kwimibhobho ziquka ukungasebenzisi i-pipette yevolumu echanekileyo kumsebenzi othile kunye nokusebenzisa i-pipette yokususa umoya ukuphatha ulwelo olungenamanzi."Ngama-viscous fluids, i-pipette ye-positive-displacement kufuneka ihlale isetyenziswa.
Ngaphambi kokuba ufikelele kwiinkqubo ezithile zokufaka imibhobho, ezinye iingcamango eziqhelekileyo kufuneka ziqwalaselwe.Ngalo lonke ixesha abasebenzisi bepipette beqala umsebenzi wosuku, kufuneka baqwalasele ukuba loluphi uvavanyo abalwenzayo, loluphi ulwelo abasebenza ngalo, kwaye banqwenela ntoni na ngaphambi kokukhetha ipipette,” utshilo uPorecha."Ngokwenyani, akukho labhu inazo zonke iipipettes ezinokufunwa ngumsebenzisi, kodwa ukuba umsebenzisi ujonga ukuba zeziphi izixhobo ezikhoyo kwilebhu nakwisebe, banokufumana umbono ongcono wokuba zeziphi iipipettes ezikhoyo ekufuneka ziphunyezwe kuvavanyo okanye kwi-assay. zeziphi iipipettes abanokufuna ukuzithenga. "
Iimpawu ezikhoyo kwiipayipi zanamhlanje zandisa ngaphaya kwesixhobo ngokwaso.Ukuqhubela phambili ekuphatheni ulwelo kwenze ukuba abasebenzisi ngoku badibanise i-pipette yabo efini.Ngolu qhagamshelo, umsebenzisi unokukhuphela iiprothokholi okanye enze ezi siko.Idatha yokufaka imibhobho inokuthi ifakwe kwilifu, enye yeendlela zokuchonga naziphi na iindlela eziphosakeleyo kunye nokuphucula inkqubo yokufaka imibhobho, ngakumbi ngokulandela umkhondo wokuchaneka okuqhubekayo, okanye ukungabikho kwayo.
Ngezixhobo ezifanelekileyo esandleni, umngeni olandelayo kukufumana amanyathelo afanelekileyo.
Isitshixo seMpumelelo
Ngombhobho wokufuduswa komoya, la manyathelo alandelayo anyusa amathuba okulinganisa ngokuchanekileyo nangokuphindaphindiweyo umthamo othile:
- Beka ivolumu kwipipette.
- Cinezela i-plunger.
- Gxininisa i-tip kubunzulu obuchanekileyo, obunokwahluka nge-pipette kunye ne-tip, kwaye ngokukhawuleza uvumele i-plunger ihambe kwindawo yayo yokuphumla.
- Linda malunga nesekondi enye ukuze ulwelo lungene kwiingcebiso.
- Beka i-pipette-ebanjwe kwi-10-45 degrees-nxamnye nodonga lwegumbi lokumkela, kwaye ucinezele ngokucokisekileyo i-plunger ukuya kwindawo yokuqala.
- Lindela umzuzwana omnye kwaye emva koko ucinezele iplunger ukuya kwindawo yesibini.
- Slayida incam eludongeni lwenqanawa ukususa i-pipette.
- Vumela iplunger ibuyele kwindawo yayo yokuphumla.
Ixesha lokuposa: Dec-12-2022