Ochwepheshe baselabhorethri bangachitha amahora usuku ngalunye bephethe i-micropipette, futhi ukuthuthukisa ukusebenza kahle kwamapayipi kanye nokuqinisekisa imiphumela enokwethenjelwa ngokuvamile kuyinselele.Ukukhetha i-micropipette efanele yanoma yisiphi isicelo kuyisihluthulelo sempumelelo yomsebenzi waselabhorethri;akuqinisekisi ukusebenza kwanoma yikuphi ukuhlola kuphela, kodwa futhi kwandisa ukusebenza kahle.Ukuqonda izidingo zokugeleza kwamapayipi kwenza abasebenzisi bakwazi ukukhetha amapayipi anembile naphindaphindwayo, kodwa kunezici eziningi okufanele zicatshangelwe ukuze kuthuthukiswe imiphumela yepayipi kanye nokuqinisekisa impumelelo yamapayipi. ukuhlola.
Ngobubanzi, uketshezi luwela ezigabeni ezintathu eziyinhloko: ezinamanzi, i-viscous, kanye ne-volatile.Uketshezi oluningi lusekelwe emanzini, okwenza amapayipi okuhambisa umoya abe yisinqumo sokuqala kwabaningi.Nakuba uketshezi oluningi lusebenza kahle nalolu hlobo lwe-pipette, amapayipi e-volumetric kufanele akhethwe uma ukusebenza nge-viscous kakhulu noma uketshezi oluguquguqukayo.Umehluko phakathi kwalezi zinhlobo ze-pipette uboniswa kuMfanekiso 1.Kubalulekile futhi ukusebenzisa indlela efanele yokufaka amapayipi - kungakhathaliseki ukuthi hlobo luni lwamanzi - ukuze uthole imiphumela emihle kakhulu.
Imingcele emibili ebaluleke kakhulu ethinta imiphumela ye-pipetting ukunemba nokunemba (Umfanekiso 2). Ukuze kuzuzwe ukunemba okuphezulu kwamapayipi, ukunemba, nokuthembeka, imibandela ethile kufanele ikhunjulwe.Njengomthetho wesithupha, umsebenzisi kufanele ahlale ekhetha i-piette encane kunazo zonke. engakwazi ukuphatha ivolumu yokudlulisa oyifunayo.Lokhu kubalulekile njengoba ukunemba kuncipha njengoba ivolumu emisiwe isondela kumthamo omncane we-pipette.Isibonelo, uma ukhipha u-50 µl nge-piette engu-5,000 µl, imiphumela ingase ibe mibi. Imiphumela engcono ingaba ngcono. etholwe ngamapayipi angu-300 µl, kuyilapho ama-pipette angu-50 µl enikeza imiphumela engcono kakhulu. Ngaphezu kwalokho, ivolumu elisethwe kumapayipi avamile angase ashintshe phakathi ne-pipetting ngenxa yokuzungezisa ngephutha i-plunger. Yingakho abanye abakhiqizi be-pipette benze imiklamo yokulungisa ivolumu yokukhiya ukuze bagweme. izinguquko ezingaqondile ngenkathi i-pipette iqhubeka nokuqinisekisa ukunemba.Ukulinganisa kungenye into ebalulekile esiza ukuqinisekisa imiphumela enokwethenjelwa ngokubonisa ukunemba nokunemba kwe-pipette.Le nqubo kufanele ibe lula kumsebenzisi;isibonelo, amanye amapayipi kagesi angasetha izikhumbuzi zokulinganisa, noma alondoloze umlando wokulinganisa.Akuwona nje amapayipi okufanele acatshangelwe.Uma ithiphu lepayipi lixega, livuze, noma liwe, lingabangela izinkinga ezihlukahlukene.Le nkinga evamile elebhu kuvame ukubangelwa ukusetshenziswa kwamathiphu e-pipette enhloso evamile, ngokuvamile adinga “ukuthepha.”Le nqubo yelula unqenqema lwethiphu ye-pipette futhi ingabangela ukuthi ichopho livuze noma lilahlekele, noma libangele nokuthi ichopho liwe ngokuphelele. .Ukukhetha i-micropipette yekhwalithi ephezulu eklanywe ngamathiphu athile kuqinisekisa ukuxhumana okuvikeleke kakhudlwana, okuhlinzeka ngezinga eliphezulu lokuthembeka nemiphumela engcono.Ukwengeza, into elula njengama-pipette anekhodi yombala namathiphu angasiza abasebenzisi ukuqinisekisa ukuthi amathiphu afanele akhethelwe amapayipi abo.
Esimweni esisezingeni eliphezulu, kubalulekile ukusebenza kahle ngangokunokwenzeka ngenkathi kugcinwa ukwethembeka nokuvumelana kwenqubo yepayipi.Kunezindlela eziningi zokuthuthukisa ukusebenza kahle kwamapayipi, okuhlanganisa ukusetshenziswa kwe-multichannel kanye/noma amapayipi kagesi.Lezi zinsimbi eziguquguqukayo ngokuvamile inikeza izindlela eziningana ezihlukene zamapayipi—ezifana nokuhlehla kwamapayipi, ukusabalalisa okuguquguqukayo, ukuhlanjululwa kwe-serial okuhleliwe, nokunye okwengeziwe—ukwenza inqubo ibe lula.Ngokwesibonelo, izinqubo ezifana nokukhipha okuphindaphindiwe zilungele ukunikeza ama-aliquot amaningi wevolumu efanayo ngaphandle kokugcwalisa ithiphu. Ukusebenzisa ama-pipette esiteshi esisodwa ukudlulisa amasampula phakathi kwamafomethi ahlukene e-labware kungashesha ukukhathaza kakhulu futhi kube namaphutha.Amapayipi e-Multichannel avumela ukudlulisa amasampula amaningi ngesikhathi esisodwa ngokucwayiza kweso. amaphutha kanye nokulimala okuphindaphindayo (i-RSI).Amanye ama-pipette anekhono lokushintsha isikhala samathiphu ngesikhathi se-pipetting, okuvumela ukudluliswa okuhambisanayo kwamasampuli amaningi phakathi kosayizi namafomethi welebhu ehlukene, ukonga amahora wesikhathi (Umfanekiso 3).
Ochwepheshe baselabhorethri ngokuvamile bachitha amahora ngosuku betting ipayipi.Lokhu kungabangela ukungakhululeki futhi, ezimweni ezimbi kakhulu, ngisho nokulimala kwesandla noma kwengalo.Iseluleko esingcono kakhulu sokugwema lezi zingozi ezingase zibe khona ukunciphisa isikhathi obamba i-pipette esikhathini esifushane kakhulu. .Ngaphezu kwalokhu, abasebenzisi kufanele bakhethe i-micropipette engasindi nebhalansiswe kahle enesisindo phakathi nendawo ukuze izinze kangcono.I-pipette kufanele ilingane kahle ezandleni zabasebenzisi besandla sobunxele nakwesokudla, ibe nomklamo omuhle wokubamba, futhi ilungise. ivolumu ngokunethezeka nangokushesha ngangokunokwenzeka ukuze ugweme ukunyakaza okungadingekile.Futhi, amathiphu abalulekile, njengoba ukulayisha ithiphu kanye nokukhipha ngokuvamile kudinga amandla angaphezu kwepayipi futhi kunengozi engaba khona yokulimala, ikakhulukazi kuzilungiselelo eziphezulu.Amathiphu we-Pipette kufanele avele endaweni ngamandla amancane, hlinzeka ngoxhumano oluvikelekile, futhi kube lula ngokulinganayo ukukukhipha.
Lapho ukhetha i-micropipette efanele yohlelo lwakho lokusebenza, kubalulekile ukubheka zonke izici zokuhamba komsebenzi wakho.Ngokucabangela i-pipette, izici zayo, uhlobo nomthamo woketshezi olufakwa ngamapayipi, namathiphu asetshenziswayo, ososayensi bangaqinisekisa ukuthi kunembe, kunembe futhi kunokwethenjelwa. imiphumela ngenkathi kugcinwa ukukhiqiza kanye nokunciphisa ingozi yokulimala.
Kulolu shicilelo, ukutholwa kokuhlaziya okuyisisekelo kuhlolwa yi-HPLC-MS kusetshenziswa ama-microplates e-SPE wemodi exubile ye-cation exchange.Izinzuzo ze-SEC-MALLS kuzicelo ze-biopharmaceutical…
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Isikhathi sokuthumela: Jun-10-2022