Cov kws tshaj lij hauv chav kuaj tuaj yeem siv sij hawm txhua hnub los tuav lub micropipette, thiab txhim kho pipetting efficiency thiab ua kom ntseeg tau cov txiaj ntsig zoo feem ntau yog ib qho nyuaj.Kev xaiv lub micropipette txoj cai rau txhua daim ntawv thov yog qhov tseem ceeb rau kev ua tiav ntawm kev ua haujlwm hauv chav kuaj;nws tsis tsuas yog ua kom cov kev ua tau zoo ntawm ib qho kev sim, tab sis kuj nce efficiency.Kev nkag siab txog cov kev xav tau ntawm pipetting workflow enables cov neeg siv xaiv qhov raug thiab rov ua dua pipettes, tab sis muaj ntau lwm yam uas yuav tsum tau txiav txim siab los txhim kho pipetting tau thiab lav qhov kev vam meej ntawm kev sim.
Kev hais lus dav dav, cov kua dej poob rau hauv peb pawg tseem ceeb: aqueous, viscous, thiab volatile.Feem ntau cov kua yog dej-raws li, ua rau huab cua hloov pipettes thawj xaiv rau ntau.Thaum cov kua feem ntau ua haujlwm zoo nrog hom pipette, volumetric pipettes yuav tsum tau xaiv thaum ua hauj lwm nrog cov kua nplaum los yog volatile heev.Qhov sib txawv ntawm cov pipette hom no muaj nyob rau hauv daim duab 1.Nws kuj tseem ceeb heev uas yuav tsum tau siv cov txheej txheem pipetting kom raug - tsis hais cov kua hom - kom tau zoo heev.
Qhov ob qhov tseem ceeb tshaj plaws uas cuam tshuam rau cov txiaj ntsig pipetting yog qhov tseeb thiab qhov tseeb (Daim duab 2). Kom ua tiav qhov siab tshaj plaws pipetting raug, precision, thiab kev ntseeg siab, ntau cov txheej txheem yuav tsum tau khaws cia hauv siab. uas tuaj yeem tswj tau qhov xav tau hloov ntim.Qhov no yog qhov tseem ceeb raws li qhov tseeb txo qis raws li qhov ntim ntim nce mus txog qhov tsawg kawg nkaus ntim ntawm cov pipette.Piv txwv li, yog tias koj faib 50 µl nrog 5,000 µl pipette, cov txiaj ntsig yuav tsis zoo. Cov txiaj ntsig zoo tuaj yeem yog tau nrog 300 µl pipettes, thaum 50 µl pipettes muab cov txiaj ntsig zoo tshaj plaws. Tsis tas li ntawd, lub ntim ntim ntawm cov phau ntawv phau ntawv pipettes tuaj yeem hloov pauv thaum lub raj xa dej vim kev sib hloov ntawm lub plunger.Qhov no yog vim li cas qee cov tuam txhab pipette tau tsim cov khoom ntim ntim kev hloov kho kom tiv thaiv. inadvertent hloov thaum pipetting kom paub tseeb tseeb.Calibration yog lwm yam tseem ceeb uas yuav pab tau guarantee txhim khu kev qha cov txiaj ntsig los ntawm kev qhia qhov tseeb thiab precision ntawm pipette.Qhov txheej txheem no yuav tsum yooj yim rau cov neeg siv;Piv txwv li, qee cov khoom siv hluav taws xob tuaj yeem teeb tsa cov lus ceeb toom calibration, lossis txuag calibration keeb kwm.Nws tsis yog pipettes xwb los xav txog.Yog tias lub pipette taub hau xoob, xau, los yog ntog, nws tuaj yeem ua rau ntau yam teeb meem.Qhov teeb meem no tshwm sim hauv chav kuaj. Feem ntau yog tshwm sim los ntawm kev siv cov lus qhia dav dav dav dav, uas feem ntau xav tau "tapping." Cov txheej txheem no nthuav tawm ntawm ntug ntawm lub taub dej thiab tuaj yeem ua rau lub taub hau los yog qhov chaw tsis zoo, lossis tseem ua rau lub taub hau poob ntawm lub pipette nkaus. .Kev xaiv lub micropipette zoo uas tsim los nrog cov lus qhia tshwj xeeb ua kom muaj kev sib txuas ruaj ntseg dua, muab qib siab dua ntawm kev ntseeg siab thiab tau txais txiaj ntsig zoo dua. Tsis tas li ntawd, qee yam yooj yim xws li cov xim coding pipettes thiab cov lus qhia kuj tuaj yeem pab cov neeg siv kom paub meej cov lus qhia raug xaiv rau lawv pipettes.
Nyob rau hauv ib puag ncig high-throughput, nws yog ib qho tseem ceeb kom ua tau raws li qhov ua tau zoo thaum tswj kev ntseeg tau thiab sib xws ntawm cov txheej txheem pipetting.Muaj ntau txoj hauv kev los txhim kho pipetting efficiency, nrog rau kev siv ntau channel thiab/los yog hluav taws xob pipettes.Cov twj paj nruag ntau yam. feem ntau muaj ntau yam kev sib txawv pipetting-xws li thim rov qab pipetting, hloov pauv hloov pauv, programmed serial dilutions, thiab ntau dua-kom yooj yim rau cov txheej txheem.Piv txwv li, cov txheej txheem xws li rov xa rov qab yog qhov zoo tshaj plaws rau kev xa tawm ntau yam aliquots ntawm tib lub ntim yam tsis tau rov qab cov lus qhia. Siv ib leeg-channel pipettes los hloov cov qauv ntawm cov qauv sib txawv ntawm labware tuaj yeem ua tau nrawm heev thiab ua yuam kev.Multichannel pipettes tso cai hloov ntau cov qauv ib zaug hauv ib lub qhov muag.Tsis tsuas yog ua kom muaj txiaj ntsig zoo, nws kuj tseem pab tiv thaiv pipetting. Kev ua yuam kev thiab rov ua dua kev raug mob (RSI).Qee lub pipettes txawm muaj peev xwm sib txawv qhov sib txawv ntawm qhov sib txawv thaum lub sij hawm pipetting, tso cai rau kev hloov mus rau tib lub sijhawm ntawm ntau qhov sib txawv ntawm qhov ntau thiab tsawg labware, txuag sijhawm sijhawm (Daim duab 3).
Cov kws tshaj lij hauv chav kuaj feem ntau siv sijhawm ntau teev hauv ib hnub pipetting.Qhov no tuaj yeem ua rau tsis xis nyob thiab, nyob rau hauv cov xwm txheej hnyav dua, txawm tias txhais tes lossis caj npab raug mob.Cov lus qhia zoo tshaj plaws kom tsis txhob muaj qhov pheej hmoo no yog txo lub sijhawm koj tuav lub pipette mus rau lub sijhawm luv tshaj plaws. . Ntxiv rau qhov no, cov neeg siv yuav tsum xaiv lub teeb yuag thiab zoo sib npaug micropipette nrog huab hwm coj nyob rau hauv qhov chaw kom muaj kev ruaj ntseg zoo dua.Lub pipette yuav tsum haum zoo nyob rau hauv ob txhais tes ntawm cov neeg siv sab laug thiab sab xis, muaj kev tuav zoo tsim, thiab kho. Lub ntim tau yooj yim thiab sai li sai tau kom tsis txhob muaj kev txav txav tsis tsim nyog.Tsis tas li, cov lus qhia tseem ceeb, raws li cov lus qhia kev thauj khoom thiab ejection feem ntau yuav tsum tau muaj zog ntau dua li pipetting thiab muaj feem yuav raug mob, tshwj xeeb tshaj yog nyob rau hauv high-throughput settings.Pipette tswv yim yuav tsum snap. nyob rau hauv qhov chaw nrog tsawg kawg nkaus quab yuam, muab kev ruaj ntseg kev twb kev txuas, thiab yuav sib npaug zos yooj yim eject.
Thaum xaiv qhov zoo micropipette rau koj daim ntawv thov, nws yog ib qho tseem ceeb uas yuav tau saib xyuas txhua yam ntawm koj qhov kev ua haujlwm.Los ntawm kev xav txog cov pipette, nws cov yam ntxwv, hom thiab ntim ntawm cov kua uas tau pipetted, thiab cov lus qhia siv, cov kws tshawb fawb tuaj yeem lav qhov tseeb, meej thiab txhim khu kev qha. tshwm sim thaum tuav cov khoom tsim tau thiab txo qhov kev pheej hmoo ntawm kev raug mob.
Hauv tsab ntawv no, qhov kev rov qab los ntawm cov tshuaj ntsuam xyuas yooj yim raug soj ntsuam los ntawm HPLC-MS siv cov sib xyaw-hom muaj zog cation txauv SPE microplates.Cov txiaj ntsig ntawm SEC-MALLS hauv kev siv biopharmaceutical…
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Lub sij hawm xa tuaj: Jun-10-2022