Precautions for laboratory pipette tips

1. Use suitable pipetting tips:
In order to ensure better accuracy and precision, it is recommended that the pipetting volume be within the range of 35%-100% of the tip.

2. Installation of the suction head:
For most brands of pipettes, especially multi-channel pipettes, it is not easy to install the Pipette tip: in order to pursue a good seal, you need to insert the pipette handle into the tip and then turn it left and right or shake it forward and backward. Tighten. There are also people who use the pipette to repeatedly hit the tip to tighten it, but this operation will cause the tip to deform and affect the accuracy. In severe cases, the pipette will be damaged, so such operations should be avoided.

3. The immersion angle and depth of the pipette tip:
The immersion angle of the tip should be controlled within 20 degrees, and it is better to keep it upright; the tip immersion depth is recommended as follows:
Pipette specification tip immersion depth
2L and 10 L 1 mm
20L and 100 L 2-3 mm
200L and 1000 L 3-6 mm
5000 L and 10 mL 6-10 mm

4. Rinse the pipette tip:
For samples at room temperature, tip rinsing can help improve accuracy; but for samples with high or low temperature, tip rinsing will reduce the accuracy of the operation. Please pay special attention to users.

5. Liquid suction speed:
The pipetting operation should maintain a smooth and appropriate suction speed; too fast aspiration speed will easily cause the sample to enter the sleeve, causing damage to the piston and seal ring and cross-contamination of the sample.

1. Maintain the correct posture when pipetting; do not hold the pipette tightly all the time, use a pipette with a finger hook to help relieve hand fatigue; change hands frequently if possible.
2. Regularly check the sealing condition of the pipette. Once it is found that the seal is aging or leaks, the sealing ring must be replaced in time.
3. Calibrate the pipette 1-2 times a year (depending on the frequency of use).
4. For most pipettes, a layer of lubricating oil should be applied to the piston before and after use for a period of time to maintain tightness.

Post time: Aug-09-2022