okubalulekile okufanele kunakwe lapho uvala inzaloAmathiphu we-Pipette?Ake sibheke ndawonye.
1. Nciphisa ithiphu ngephephandaba
Yifake ebhokisini lethiphu ukuze uvale inzalo yokushisa okumanzi, ama-degree angu-121, ukucindezela komkhathi ongu-1bar, imizuzu engu-20;ukuze ugweme inkinga yomhwamuko wamanzi, ungagoqa ibhokisi lethiphu ngephephandaba, noma ulibeke ku-incubator ngemva kokuvalwa inzalo ukuze lome.
2. Lapho i-autoclaving, ibhokisi lethiphu kufanele ligoqwe ephephandabeni ukuze kubulawe inzalo
Ukusonga kwamaphephandaba kungamunca amanzi futhi kungagwema amanzi amaningi, into ebaluleke kakhulu ukuvimbela ukungcoliswa kabusha.
3. Izindaba ezidinga ukunakwa ekufakweni inzalo kwamathiphu e-pipette ngesikhathi sokukhishwa kwe-RNA
Sebenzisa amashubhu e-EP ajwayelekile namathiphu we-pipette.Ngaphambi kokwenza i-autoclaving, zicwilise emanzini e-DEPC ubusuku bonke ukuze ususe i-RNase.Ngemva kokukhipha i-DEPC ngosuku olulandelayo, zifake ebhokisini lethiphu ye-pipette ukuze uvale inzalo yokushisa okumanzi.121 degrees, 15-20 imizuzu.Ukuze ugweme izinkinga zomhwamuko wamanzi, amaphephandaba angasongwa ebhokisini lethiphu, noma afakwe ku-incubator ukuze ome ngemva kokuvalwa inzalo.Kungcono ukuvala inzalo ngaphambi kokukhipha ngakunye, futhi ungasebenzisi amathiphu e-pipette ahlala isikhathi eside ukuze ukhiphe i-RNA.
Izinzuzo zokuvala inzalo ngezinga lokushisa eliphezulu:
Ukungena kokushisa kwe-steam okunamandla;ukusebenza kahle kokuvala inzalo;isikhathi esincane sokuvala inzalo;akukho ukungcoliswa kwamakhemikhali noma ngokomzimba phakathi nenqubo yokuvala inzalo;imingcele embalwa yokulawula yezinto zokusebenza zokuvala inzalo nokusebenza okuzinzile;inzalo yesitimu isetshenziselwa ukonga amanzi namandla.Ukusebenza kahle okuphezulu kokushisa.
Amathiphu we-pipette ka-Yongyue enziwe nge-polypropylene (PP) yebanga lezokwelapha, ehlangabezana nebanga le-USP VI, inokumelana namakhemikhali okuhle kakhulu, futhi ingafakwa inzalo ku-121 degrees izinga lokushisa eliphezulu kanye nokucindezela okuphezulu (ukwelashwa okujwayelekile kwe-electron beam sterilization).
1. Nciphisa ithiphu ngephephandaba
Yifake ebhokisini lethiphu ukuze uvale inzalo yokushisa okumanzi, ama-degree angu-121, ukucindezela komkhathi ongu-1bar, imizuzu engu-20;ukuze ugweme inkinga yomhwamuko wamanzi, ungagoqa ibhokisi lethiphu ngephephandaba, noma ulibeke ku-incubator ngemva kokuvalwa inzalo ukuze lome.
2. Lapho i-autoclaving, ibhokisi lethiphu kufanele ligoqwe ephephandabeni ukuze kubulawe inzalo
Ukusonga kwamaphephandaba kungamunca amanzi futhi kungagwema amanzi amaningi, into ebaluleke kakhulu ukuvimbela ukungcoliswa kabusha.
3. Izindaba ezidinga ukunakwa ekufakweni inzalo kwamathiphu e-pipette ngesikhathi sokukhishwa kwe-RNA
Sebenzisa amashubhu e-EP ajwayelekile namathiphu we-pipette.Ngaphambi kokwenza i-autoclaving, zicwilise emanzini e-DEPC ubusuku bonke ukuze ususe i-RNase.Ngemva kokukhipha i-DEPC ngosuku olulandelayo, zifake ebhokisini lethiphu ye-pipette ukuze uvale inzalo yokushisa okumanzi.121 degrees, 15-20 imizuzu.Ukuze ugweme izinkinga zomhwamuko wamanzi, amaphephandaba angasongwa ebhokisini lethiphu, noma afakwe ku-incubator ukuze ome ngemva kokuvalwa inzalo.Kungcono ukuvala inzalo ngaphambi kokukhipha ngakunye, futhi ungasebenzisi amathiphu e-pipette ahlala isikhathi eside ukuze ukhiphe i-RNA.
Izinzuzo zokuvala inzalo ngezinga lokushisa eliphezulu:
Ukungena kokushisa kwe-steam okunamandla;ukusebenza kahle kokuvala inzalo;isikhathi esincane sokuvala inzalo;akukho ukungcoliswa kwamakhemikhali noma ngokomzimba phakathi nenqubo yokuvala inzalo;imingcele embalwa yokulawula yezinto zokusebenza zokuvala inzalo nokusebenza okuzinzile;inzalo yesitimu isetshenziselwa ukonga amanzi namandla.Ukusebenza kahle okuphezulu kokushisa.
Amathiphu we-pipette ka-Yongyue enziwe nge-polypropylene (PP) yebanga lezokwelapha, ehlangabezana nebanga le-USP VI, inokumelana namakhemikhali okuhle kakhulu, futhi ingafakwa inzalo ku-121 degrees izinga lokushisa eliphezulu kanye nokucindezela okuphezulu (ukwelashwa okujwayelekile kwe-electron beam sterilization).
Isikhathi sokuthumela: Nov-02-2021