I-polymerase chain reaction (PCR) iyindlela esetshenziswa kakhulu abacwaningi be-biomedical, ososayensi bezobunhloli kanye nochwepheshe bamalabhorethri ezokwelapha.
Ibala ukusetshenziswa kwayo okumbalwa, isetshenziselwa ukwenza i-genotyping, ukulandelana, i-cloning, nokuhlaziya isisho sofuzo.
Nokho, ukulebula amashubhu e-PCR kunzima ngoba mancane futhi anendawo encane yokugcina ulwazi.
Nakuba, amapuleti e-PCR (qPCR) e-skirted quantitative angalebula ohlangothini olulodwa kuphela.
Ingabe udinga isikhathi eside, esiqinile I-PCR tubeukuze usebenzise elabhorethri yakho?Zama ukusekela umkhiqizi owaziwayo.
Iphakheji Eliphelele
I-PCR-Tag Trax esalinde ilungelo lobunikazi iyinketho yakamuva kakhulu nengcono kakhulu yokulebula amashubhu e-PCR ephrofayili ephezulu, imicu, kanye namaplatifomu e-qPCR.
Idizayini eguquguqukayo yethegi enganamatheli iyenza ikwazi ukuhlonza amashubhu e-PCR ephrofayili ephezulu angu-0.2 ml namapuleti e-qPCR angagqoke iziketi ekucushweni okuhlukahlukene.
Inzuzo enkulu ye-PCR-Tag Trax yikhono layo lokuhlinzeka ngenani eliphelele lesikhala sokuphrinta noma, uma kunesidingo, ukubhala ngesandla.
Ngokusebenzisa iphrinta yokudlulisa eshisayo, amathegi angaphrintwa ngezinombolo ze-serialized kanye ne-1D noma amabhakhodi we-2D futhi angamelana nezinga lokushisa eliphansi elifinyelela ku--196°C futhi lifike ku-+150°C.
Lokhu kuwenza avumelane namabhayisikili amaningi e-thermo.Kungumqondo omuhle ukuhlola isampula yomaka kumabhayisikili akho e-thermo ukuze uqinisekise ukuthi awaphazamisi ukusabela.
Kufanele alungele amagilavu, anikeze umbono wenyoni ngokushesha wolwazi olubhalwe kumathegi uma sekuvulwe amabhayisikili e-thermo.
Amashubhu e-PCR angase abe nombala ohlukahlukene noma ifomethi enemibala eminingi ukuze kube lula ukulebula ngombala.
Amathegi anganamatheli angasetshenziswa futhi njengokweseka amashubhu akho, okwenza kube lula ukufaka ama-reagents e-pipette kuwo futhi uwagcine efrijini noma efrijini ngemva kokusabela.
Amashubhu e-PCR, 0.2mL
Amashubhu e-PCR angawodwana angalebula ezindaweni ezimbili ezihlukene: amashubhu nekepisi lawo.
Ukuze kube lula ukufaka ikhodi yombala, amalebula aseceleni amashubhu amancane e-PCR atholakala ngenani lemibala yawo womabili amaphrinta e-laser nawe-thermal-transfer.
Ulwazi olwengeziwe lungaphrintwa kulawa malebula eshubhu le-PCR kunolo olungabhalwa ngesandla, futhi amabhakhodi angasetshenziswa ukuthuthukisa ukulandeleka.
Amalebula aphephile futhi angagcinwa eziqandisini zelebhu isikhathi eside.
Amalebula amachashazi ayindilinga ayinketho engcono kakhulu yokulebula iziqongo zamashubhu e-PCR.
Amalebula amachashazi, ngakolunye uhlangothi, anenani elilinganiselwe lendawo kushubhu ukuze aphrinte noma abhale ulwazi.Ngakho-ke ukuwenza abe ngenye yezinketho zokulebula zamashubhu e-PCR asebenza kancane.
Uma kufanele usebenzise amalebula amachashazi kumashubhu e-PCR futhi uzobe ulebula inani elikhulu lawo, i-pikaTAGTM.
I-pikaTAGTM iyithuluzi lohlelo lokusebenza elithatha amalebula amachashazi ngokuqondile kulayini wawo bese liwanamathisela phezulu kwamashubhu.
Inefomu elifana nepeni le-ergonomic elenza ukulebula kwamachashazi kusheshe futhi kube lula, kususe umsebenzi odla isikhathi wokukhetha amalebula amancane kanye nokuvimbela ukulimala kwengcindezi okubangelwa ukulebula kweshubhu.
Imichilo Yamashubhu e-PCR
Imichilo ye-PCR ivame ukusetshenziswa kumalebhu asebenzisa izinqubo eziningi ze-PCR kanye ne-qPCR.
Ukulebula le micu kuyinselele nakakhulu kunokulebula amashubhu ngamanye ngoba ishubhu ngalinye lixhunywe kwelilandelayo, ngaleyo ndlela kuncipha indawo ehlonza kakade ekhawulelwe kakade.
Ngenhlanhla, imicu yelebula yamashubhu angu-8 ihambisana neshubhu ngalinye, okwenza umugqa we-PCR ulebula umoya ohelezayo.
Le micu esungulwe i-GA international, inezimbobo phakathi kwelebula ngayinye kumqulu, okukuvumela ukuthi uphrinte amalebula amaningi njengoba kunamashubhu.
Beka wonke umugqa welebula eduze kohlangothi lweshubhu, unamathisele wonke amalebula ngesikhathi esifanayo, bese uphula ama-perforations ukuze ugcine amalebula enamathele ngokuqinile ohlangothini.
Ezingeni lokushisa elingu--80°C kuya ku-+100°C, lawa malebula aphrintekayo okudluliselwa kwe-thermal aphephile ukuthi angasetshenziswa kumabhayisikili e-thermo futhi angase agcinwe ngokuphephile kumafriji aselabhorethri.
Indlela Yendabuko
Ukubhala ngesandla kuyindlela evamile kakhulu yokuhlonza amashubhu e-PCR, nakuba kukude kakhulu nokuhle ngoba ukubhala ngokufundekayo kumashubhu e-PCR cishe akunakwenzeka.
Ukubhala ngesandla kuphinde kuqede ukwenza uchungechunge namabhakhodi, okwenza kube nzima kakhulu ukulandelela amasampula akho.
Uma ukubhala ngesandla kuwukuphela kwenketho ngelebhu yakho, izimpawu ze-cryo zethiphu enhle kufanele utshale kuzo njengoba zikuvumela ukuthi ubhale ngendlela efundekayo ngaphandle kokufiphala noma ukufiphala.
Xhumana nathi ukuze uthole ama-PCR Tubes asezingeni eliphezulu
Sakha futhi sikhiqize izinga eliphezuluAmashubhu e-PCRukuze zisetshenziswe ku-genotyping, ukulandelana, ukuhlanganisa, nokuhlaziya izakhi zofuzo kumalabhorethri ezokwelapha ahlukahlukene kanye nesikhungo socwaningo.
Ukuze uthole ulwazi olungcono kakhulu ngamashubhu e-PCR, yenzafinyelela kithi ukuthola umkhiqizo wekhwalithi nokusebenza kahle.
Isikhathi sokuthumela: Oct-30-2021