Urwego rwa polymerase reaction (PCR) nuburyo bukoreshwa cyane nabashakashatsi ku binyabuzima, umuhanga mu by'amategeko n’inzobere muri laboratoire z'ubuvuzi.
Kubara bike mubikorwa byayo, ikoreshwa mugukoresha genotyping, ikurikiranye, cloni, no gusesengura imvugo ya gene.
Ariko, gushyira ibirango bya PCR biragoye kuko ni bito kandi bifite umwanya muto wo kubika amakuru.
Mugihe, plaque yuzuye PCR (qPCR) isahani ishobora gushyirwaho gusa kuruhande rumwe
Ukeneye igihe kirekire, gikomeye Umuyoboro wa PCRgukoreshwa muri laboratoire yawe?Iyemeze gushigikira uruganda ruzwi.
Amapaki yose
Ipatanti itegereje PCR-Tag Trax nuburyo bwa vuba kandi bwiza bwo gushyiramo ikirango cya PCR cyamamaye cyane, imirongo, hamwe na qPCR.
Ikirangantego kidahuye nigishushanyo mbonera gishobora kugufasha kumenya 0.2 ml yo hejuru ya PCR ya PCR hamwe na plaque ya qPCR idafite amajipo muburyo butandukanye.
Inyungu nyamukuru ya PCR-Tag Trax nubushobozi bwayo bwo gutanga umwanya mwiza wo gucapa cyangwa, nibiba ngombwa, kwandika intoki.
Ukoresheje icapiro ryimyanya yumuriro, tagi irashobora gucapishwa numero ikurikirana kimwe na barcode ya 1D cyangwa 2D kandi irashobora kwihanganira ubushyuhe buri munsi ya -196 ° C kandi hejuru ya + 150 ° C.
Ibi bituma bahuza nabamotari benshi.Nibyiza ko ugerageza icyitegererezo cya tagi mumagare yawe bwite ya thermo kugirango umenye neza ko atabangamira reaction.
Bagomba kuba bafite uturindantoki, batanga inyoni yihuta yijisho ryamakuru yanditse kuri tagi iyo gare ya thermo imaze gufungurwa.
Imiyoboro ya PCR irashobora kuza muburyo butandukanye bwamabara cyangwa imiterere yamabara menshi kugirango byoroshye amabara.
Ibirango bidafite ibifatika birashobora kandi gukoreshwa nkinkunga yigituba cyawe, bigatuma byoroha kubisubiramo reagent hanyuma ukabibika muri frigo cyangwa firigo nyuma yo kubyitwaramo.
Imiyoboro ya PCR, 0.2mL
Imiyoboro ya PCR kugiti cye irashobora gushyirwaho hejuru yuburyo bubiri butandukanye: igituba nigitambara cyacyo.
Kuburyo bworoshye bwo kubara, ibirango byuruhande rwa tike ya PCR iraboneka mumibare yamabara yombi ya laser na printer-yumuriro.
Ibisobanuro byinshi birashobora gucapurwa kuri label ya PCR ya tube kuruta iyandikishijwe intoki, kandi barcode irashobora gukoreshwa mugutezimbere.
Ibirango bifite umutekano kandi birashobora kubikwa muri firigo ya laboratoire igihe kirekire.
Akadomo kazengurutse ni amahitamo meza yo kuranga PCR tube hejuru.
Akadomo k'akadomo, kurundi ruhande, gafite umubare muto wubutaka bwo gucapa cyangwa kwandika amakuru.Kubikora rero kimwe mubintu bidakorwa neza bya PCR tubes labels amahitamo.
Niba ugomba gukoresha utudomo duto kuri PCR kandi uzaba uranga umubare munini wabyo, pikaTAGTM.
PikaTAGTM nigikoresho cyo gusaba gikuramo utudomo duto duto uhereye kumurongo wabo hanyuma ukayihuza hejuru yigituba.
Ifite ikaramu isa na ergonomique ituma utudomo twandika vuba kandi byoroshye, bivanaho akazi gatwara igihe cyo gutoranya utuntu duto no gukumira ibikomere biterwa na label.
Imirongo ya PCR
Imirongo ya PCR ikoreshwa kenshi muri laboratoire ikora inzira nyinshi za PCR na qPCR.
Gushyira akamenyetso kuri iyi mirongo biragoye kuruta gushyiramo tebes imwe kuko buri tiyo ihujwe nubutaha, bityo bikagabanya ahantu hamenyekanye.
Kubwamahirwe, imirongo ya 8-tube ya label ihuza na buri tube, bigatuma PCR yerekana ikirango cyumuyaga.
Iyi mirongo yahimbwe na GA mpuzamahanga, ifite perforasi hagati ya buri kirango mumuzingo, igufasha gucapa ibirango byinshi nkaho hariho tubes.
Shira ikirango cyose kumurongo kuruhande rwigituba, shyira ibirango byose icyarimwe, hanyuma umenagure kugirango utume ibirango bikomeza kuruhande.
Ku bushyuhe bwa dogere -80 ° C kugeza kuri + 100 ° C, ibyo birango byandika-byerekana ubushyuhe birashobora gukoreshwa mumagare ya termo kandi birashobora kubikwa neza muri firigo ya laboratoire.
Uburyo bwa gakondo
Kwandika intoki nuburyo busanzwe bwo kumenya imiyoboro ya PCR, nubwo itari nziza kuko kwandika byemewe kubituba bya PCR ntibishoboka.
Inyandiko y'intoki nayo ikuraho serialisation na barcode, bigatuma bigorana gukurikirana ingero zawe.
Niba kwandika intoki aribwo buryo bwonyine bwo guhitamo laboratoire yawe, ibimenyetso byiza bya cryo biranga agaciro ko gushora imari kuko iguha uburenganzira bwo kwandika byemewe bishoboka bitazimangana cyangwa bitavanze.
Twandikire kubijyanye na PCR nziza
Turahimba kandi tugatanga umusaruro mwizaPCR tubeskugirango ikoreshwe muri genotyping, ikurikiranye, clon, hamwe nisesengura rya gen muri laboratoire zitandukanye zubuvuzi hamwe nikigo cyubushakashatsi.
Kuburambe bwiza hamwe na PCR tubes, korashikira kuri twe kubicuruzwa byiza kandi bikora.
Igihe cyo kohereza: Ukwakira-30-2021