I-automated pipettingingenye yezindlela eziphumelela kakhulu zokunciphisa iphutha lomuntu, ukwandisa ukunemba nokunemba, nokusheshisa ukuhamba komsebenzi ngelebhu.Kodwa-ke, ukunquma ngezingxenye "okufanele ube nazo" zokuphatha uketshezi oluzenzakalelayo lokuhamba komsebenzi kuncike ezinhlosweni zakho nezinhlelo zokusebenza.Lesi sihloko sidingida amanye amaphuzu abalulekile okufanele uwacabangele lapho ukhetha inkundla yokuphatha uketshezi yelabhorethri yakho.
I-automating pipetting iyisinyathelo esibalulekile ekuthuthukiseni ukugeleza komsebenzi waselabhorethri, okusiza ekwandiseni ukukhiqizwa kabusha, ukukhulisa ukuphuma, nokunciphisa amaphutha.Amalabhorethri ancike kubuchwepheshe bokuphatha uketshezi oluzenzakalelayo ezinhlobonhlobo zezinhlelo zokusebenza, okuhlanganisa ukulungiswa kwesampula, ukukhishwa kwe-DNA, ama-assays asekelwe kumaseli, kanye nama-ELISA.Lezi zindawo ziwukutshalwa kwezimali kwesikhathi eside futhi akufanele zikhethwe ngokusekelwe ezidingweni zanamuhla kuphela, kodwa futhi nezidingo ezingaba khona zesikhathi esizayo zelebhu.Lokhu kuzoqinisekisa ukuthi kukhethwa inkundla efanele, futhi ingasebenza ngempumelelo elabhorethri iminyaka eminingi ezayo.
Izinyathelo zokuqala
Ngaphambi kokwenza noma yiziphi izinqumo, bheka kahle izinqubo ezizozenzakalela:
Ingabe uqala ngenqubo eqinile?
I-automation yokubamba uketshezi ingathuthukisa kakhulu ukuhamba komsebenzi okwenziwa ngesandla, kodwa ayikwazi ukulungisa isilingo esingakasebenzi kakade.Hlukanisa ukuhamba kwakho komsebenzi ube yizinyathelo ngazinye, bese ucabanga ngomthelela ongaba khona wento ngayinye ekuhambeni komsebenzi kukonke.Isibonelo, ukuthatha i-assay kusuka kufomethi esekelwe ngamapayipi ngesandla, esekelwe kushubhu ukuya ekugelezeni komsebenzi okuzenzakalelayo, ukuminyana okuphezulu, okusekelwe kumapuleti kusho ukuthi amasampuli nama-reagents azoba sedekhi isikhathi eside kakhulu.Lokhu kungathinta kanjani ubuqotho bamasampuli akho nama-reagents?
Izidingo zakho zizoshintsha kanjani?
Ukuze wonge imali, kungase kulingeke ukutshala imali ohlelweni oluhlangabezana nezidingo zamanje zelabhu yakho, kodwa ngokuhamba kwesikhathi ungase ulahlekelwe.Cabangela ukuthi yiziphi izakhi ezibalulekile, futhi yikuphi okungaba kuhle ukuba nazo.Uhlelo oluhle lokubamba uketshezi oluzenzakalelayo kufanele lulungiseke kabusha ukuze ukwazi ukuthatha izinhlelo zokusebenza ezintsha nokugeleza komsebenzi njengoba izidingo zishintsha.Ngesistimu eguquguqukayo, eyimojuli, izici eziningi zokugeleza komsebenzi wakho wamanje zingahlehliswa kabusha futhi zithuthukiswe.
Ingabe sikhona isixazululo esingaphandle kweshalofu esihlangabezana nezidingo zakho?
Ezinye izindawo zokusebenza eziyisipesheli zenzelwe izinhlelo zokusebenza ezithile ezinezivumelwano eziqinisekisiwe, ezifana nokukhipha i-DNA, ukulungiswa kwesampula, nesiko lamaseli.Lokhu kungase kube lula kakhulu inqubo yakho yokukhetha, futhi kusanikeze ingxenye “ebalulekile” ewusizo ukuze ihlanganiswe nesistimu enkulu ngokuzayo.Izixazululo ezingaphandle kweshalofu eziklanywe ngokuhlanganisa okuzayo nokuvumelana nezimo engqondweni zingcono kunezinkundla ezingaguquki, "ezivaliwe".
Singakanani isikhala onayo, futhi usisebenzisa kahle?
Indawo ngokuvamile iyimpahla eyigugu.Amasistimu amaningi okuphatha uketshezi manje asengabasebenzisi abaningi, okukhulise isidingo sokuguquguquka nokusetshenziswa okusha kwesikhala.Cabangela ukukhetha iplathifomu ezenzakalelayo engafinyelela isikhala esingaphansi kwethebula lokusebenza ongafinyelelwa kuyo, isibonelo, ukuhlaziya okwengeziwe noma amadivayisi okulungiselela amasampula, njll.
Kulula kangakanani ukunakekela nokusevisa?
Ungakunaki ukusevisa nokunakekela.Ukufinyelela kalula kochwepheshe kunganciphisa isikhathi nokuphazamiseka ekuhambeni kwakho komsebenzi.
Ukukhetha ihadiwe efanele
Noma ngabe usebenza ku-genomics, i-cell biology, ukutholwa kwezidakamizwa, ukuxilonga kwamangqamuzana, noma okuthile okuhluke ngokuphelele, isistimu yokuphatha uketshezi efanele ingenza impilo yakho ibe lula kakhulu.Okucatshangelwayo okubalulekile kuhlanganisa:
I-air noma i-liquid displacement pipetting?
Ukususwa komoya kulungele ukusabalalisa ngevolumu yobubanzi obukhulu, ukusuka ku-0.5 kuye ku-1,000 μL.Nakuba kuhambisana kuphela namathiphu alahlwayo, lokhu kwandisa isivinini kanye nokukhiqiza ngokususa izinyathelo ezengeziwe ezihambisana nokugudluka kwamapayipi oketshezi lapho ushintsha uketshezi noma ushayela uhlelo.Iphinde yehlise ingcuphe yokutheleleka okuphambene futhi inikeze indlela ephephile yokuphatha izinto ezikhipha imisebe noma eziyingozi.
Ukususwa koketshezi kuhambisana nawo womabili amathiphu angaguquki nalahlwayo, futhi kuwubuchwepheshe obuncanyelwayo bevolumu yokusabalalisa okuningi okungaphansi kuka-5 μL.Amathiphu ensimbi engageziwe alungele ukusetshenziswa lapho amashubhu adinga ukubhobozwa noma kudingeka i-positive pipetting.Ukuze uthole ukuguquguquka okukhulu, cabangela isistimu ehlanganisa kokubili ukususwa komoya noketshezi.
Imaphi amavolumu namafomethi osebenza nawo?
Qiniseka ukuthi inkundla ingakwazi ukuphatha amavolumu adingekayo wamapayipi namafomethi elebhu (amashubhu namapuleti) avame ukusetshenziswa elebhu yakho.Futhi cabanga ukuthi ukuzenzekelayo kuzovumela amasampula amancane namavolumu e-reagent ukuthi asetshenziswe, anikeze ukonga kwezindleko okungaba khona.
Yiziphi izingalo zamapayipi okufanele ukhethe?
Izinhlobo eziyinhloko 1) ama-pipette amashaneli aguquguqukayo—ngokuvamile angu-1- kuya ku-8-channel—angakwazi ukuphatha amashubhu, amapuleti, nezinye izakhiwo eziningi zelebhu;kanye 2) nezingalo zeziteshi eziningi eziklanyelwe ngokukhethekile ukusabalalisa kumapuleti anemithombo eminingi.Izinhlelo zesimanje zivumela amakhanda amapayipi noma amapuleti e-adaptha ukuthi ashintshwe “ngondiza”—ukukhethwa okuhlakaniphile kwezivumelwano ezisebenzisa izesekeli eziningi ezihlukene, njengezinaliti ezingaguquki, amathiphu alahlwayo, amathuluzi ophini wevolumu ephansi, njll.
Ingabe udinga izingalo zamarobhothiokweukuguquguquka okwengeziwe?
Izingalo zokubamba zerobhothi zinikeza ukuguquguquka okukhulu ngokuhambisa ilebula endaweni yokusebenza.Izingalo zamarobhothi ezingashintsha “iminwe” yazo ngokushesha ziqinisekisa ukuguquguquka okukhulu nokubamba okuphephile kwawo womabili amashubhu namapuleti.
Iluphi uhlobo lwethiphu ye-pipette oluzokwandisa ukukhiqizwa kabusha?
Ikhwalithi yethiphu inegalelo elikhulu ekukhiqizeni futhi futhi ingenza noma yephule ukusebenza kwesistimu.Amathiphu alahlwayo ngokuvamile abhekwa njengenketho engcono kakhulu yokususa ukungcoliswa okuphambene phakathi kwamasampuli ebhayoloji.Abanye abathengisi futhi manje banikeza amathiphu akhethekile evolumu ephansi aqinisekisiwe ukuze anikeze okuthembekile kumazinga we-microliter noma ama-submicroliter adingekayo ezinhlelweni ezifana ne-assay miniaturization.Cabangela ukuthenga uhlobo lomthengisi oluzenzakalelayo lwamathiphu e-pipette ukuze uqinisekise ukuthi uthola imiphumela ethembeke kakhulu.
Amathuluzi asebenzisa amathiphu angashintshi angase abe nezinzuzo ngokuphathelene nezindleko zokusebenza.Izinaliti zensimbi ezingaguquki ngokuvamile zingafinyelela phansi kwemikhumbi ejulile kangcono kunamathiphu alahlwayo, futhi zingabhoboza i-septa.Iziteshi zokugeza zamathiphu eziklanywe kahle zinciphisa ingcuphe yokungcola okuphambene nalokhu kusetha.
Ingabe udinga amathiphu aqinisekisiwe ukuthi ayinyumba?
Ukuze unciphise ubungozi bokungcola, sebenzisa kuphela izinto ezidlekayo ezilebulwe ngokuthi “inyumba.”Lawa akhiqizwa ngaphansi kwezimo eziqinile futhi ahambisana nezindinganiso zokupakisha nezokuthutha eziqinisekisa ubunyumba bethiphu yonke indlela eya ebhentshini laselabhu.Imikhiqizo ebhalwe ukuthi “presterile” ayinayo uma ishiya umkhiqizi, kodwa ihlangabezana namathuba amaningi okungcola kamuva.
Isoftware ibalulekile
Isofthiwe ihlinzeka ngokuxhumana nomuntu omisa futhi asebenzise okokusebenza, futhi ukwakheka kwayo kuzonquma ukuthi kulula kangakanani ukuhlela nokusebenzisana nesistimu ukuze ulungiselele ukuhamba komsebenzi, usethe imingcele yenqubo, futhi wenze ukukhetha kokuphatha idatha.Iphinde ibe nomthelela oqondile ekutheni kungakanani ukuqeqeshwa okudingekayo ukuze kusetshenziswe uhlelo ngokuzethemba.Ngaphandle uma unochwepheshe besofthiwe endlini, isofthiwe eklanywe kabi, kungakhathaliseki ukuthi inamandla kangakanani, ingakushiya uncike kumthengisi noma uchwepheshe wangaphandle ukuze athuthukise izimiso eziyisisekelo, ukuxazulula izinkinga, futhi enze ngisho nezinguquko ezilula zohlelo.Kumalebhu amaningi, u-opharetha wesistimu akayena uchwepheshe wokuhlela, futhi amaqembu amaningi e-IT ngeke ahileleke ngokuqondile nesofthiwe yokulawula izinsimbi.Ngenxa yalokho, kungase kudingeke ulinde kuze kube khona ababonisi bangaphandle, okuphazamisa kakhulu ukukhiqiza nokubeka izikhathi zephrojekthi engcupheni.
Amaphuzu okufanele acatshangelwe
Imibuzo ebalulekile okufanele uyibuze lapho uhlola isofthiwe yesistimu yokubamba uketshezi ihlanganisa:
- Ingabe opharetha bangasebenzisana nesikrini esithintwayo ukuze basebenze nsuku zonke?
- Ingabe umthengisi unawo umtapo wezincwadi wezivumelwano ezikhona zokwenza lula ukuhlela?
- Yimaphi amakhono okuhlanganiswa kwesofthiwe kumadivayisi ezinkampani zangaphandle?
- Ingakanani izinga lomtapo wolwazi womshayeli wedivayisi ohlinzekwa umthengisi?
- Ingabe umthengisi unolwazi nge-LIMS interfacing?
- Ungakhululeka ukuhlela uhlelo ngokwakho?
- Kulula kangakanani kuma-opharetha ukusetha izinhlelo zabo ngaphandle kobuchwepheshe bokuhlela?
- Yiziphi izici—njengemihlahlandlela yokulayisha eyizithombe—oyidingayo, futhi ingabe iyatholakala?
- Ingabe kulula ukulungisa kabusha isofthiwe lapho isistimu ihloselwe kabusha?
- Ingabe umthengisi angasiza ukuqinisekisa ukuphepha kwe-cybersecurity?
Ukulandeleka kwesampula
Ukulandelelwa kwesampula okuphelele kungabaluleka ukuze kuthotshelwe izindinganiso zekhwalithi nemihlahlandlela.Ukulebula ibhakhodi, kanye nesofthiwe efanele, kuzokwenza kube lula ukulandelelwa kwakho kokubili amasampula nezinto ezisetshenziswayo, futhi kungavimbela ukulahleka kokulandeleka.Izixazululo zokulebula ezizenzakalelayo nezokulandelela zingaphinda:
- Khomba indawo ye-labware emphemeni nasezindaweni zokugcina
- Qinisekisa ukuthi amalebula ebhakhodi asetshenziswe kahle futhi angafundwa ngendlela efanele
- Sheshisa ukufundwa kwebhakhodi nezinqubo zokukhetha amasampula, futhi wenze lula ukuhlanganiswa kwe-middleware ne-LIMS.
Inketho yokungenelela
Amaphutha enziwa kalula, kodwa akulula ngaso sonke isikhathi ukuwalungisa.Amasistimu amaningi ezishintshayo awanawo umsebenzi othi “qalisa/misa” noma “hlehlisa”, okungase kusho ukuthi kufanele uqalise kabusha uhlelo uma ufaka okuthile ngokungalungile noma udinga ukumisa inqubo.Bheka isistimu ye-automation ehlakaniphile engathola, iqonde, ibike, futhi ilulame ephutheni, enomsebenzi wokuqalisa/wokumisa ukuze uvumele ukusebenzisana komsebenzisi okuphephile nokulula nendawo yokusebenza yethuluzi phakathi nokugijima.
Isifinyezo
Ukuphatha uketshezi oluzenzakalelayo kungaqeda imisebenzi eminingi eyisicefe, kuthuthukise ukukhiqiza futhi kukhulule isikhathi esibalulekile somsebenzi obaluleke kakhulu—kodwa kuphela uma usebenzisa izixazululo ezifanele.Ukucatshangelwa ngokucophelela amaphuzu okukhulunywe ngawo kulesi sihloko kuzosiza ama-laboratories ukuba akhethe ngokuhlakanipha, okuzivumele ukuba zithole inzuzo yokusingatha uketshezi oluzenzakalelayo futhi zenze ukuphila kube lula futhi kukhiqize kakhudlwana.
Isikhathi sokuthumela: May-10-2022