Umbhobho ozenzekelayoyenye yezona ndlela zisebenzayo zokunciphisa impazamo yomntu, ukwandisa ukuchaneka kunye nokuchaneka, kunye nokukhawulezisa ukuhamba kwelabhu.Nangona kunjalo, ukwenza isigqibo malunga nezinto "ekufuneka ube nazo" zokuqhuba kakuhle kolwelo olusebenzayo kuxhomekeke kwiinjongo zakho kunye nezicelo.Eli nqaku lixoxa ngamanye amanqaku aphambili ekufuneka uwaqwalasele xa ukhetha iqonga lokuphatha ulwelo kwilabhoratri yakho.
I-automating pipetting linyathelo eliphambili ekuphuculeni ukuhamba komsebenzi webhubhoratri, ukuncedisa ukwandisa ukuveliswa kwakhona, ukunyusa ukugqithisa, kunye nokunciphisa iimpazamo.Iilebhu zixhomekeke kubuchwephesha bokuphatha ulwelo oluzenzekelayo kuluhlu oluhlukeneyo lwezicelo, kubandakanywa ukulungiswa kwesampulu, ukukhutshwa kwe-DNA, ii-assays ezisekwe kwiseli, kunye ne-ELISAs.La maqonga lutyalo-mali lwexesha elide kwaye kufuneka akhethwe ngokusekwe kungekuphela nje kwiimfuno zanamhlanje, kodwa nakwiimfuno zexesha elizayo zelebhu.Oku kuya kuqinisekisa ukuba kukhethwe iqonga elichanekileyo, kwaye linokusebenzela ngokusebenzayo ilabhoratri kwiminyaka emininzi ezayo.
Amanyathelo okuqala
Ngaphambi kokuba wenze naziphi na izigqibo, jonga kakuhle kwiinkqubo eziza kuzenzekela:
Ngaba uqala ngenkqubo eyomeleleyo?
I-automation yokusingatha ulwelo inokuphucula kakhulu ukuhamba komsebenzi ngesandla, kodwa ayinako ukulungisa i-assay engasebenziyo.Yaphula ukuhamba kwakho komsebenzi ube ngamanyathelo omntu ngamnye, kwaye ucinge ngempembelelo enokubakho yento nganye ekuhambeni komsebenzi ngokubanzi.Ngokomzekelo, ukuthatha i-assay kwi-pipetted manual, ifomathi esekelwe kwi-tube ukuya kwi-automated, high-density, i-plate-based workflows kuthetha ukuba iisampulu kunye nee-reagents ziya kuba kwidekhi ixesha elide kakhulu.Oku kunokuchaphazela njani ukuthembeka kweesampulu zakho kunye nee-reagents?
Ziya kutshintsha njani iimfuno zakho?
Ukonga imali, kusenokuhenda ukutyala imali kwinkqubo ehlangabezana neemfuno zangoku zelebhu yakho, kodwa ekuhambeni kwexesha unokuphulukana nayo.Qwalasela ukuba zeziphi izinto ezibalulekileyo, kwaye zeziphi eziya kuba kuhle ukuba nazo.Inkqubo efanelekileyo yokuphatha ulwelo oluzenzekelayo kufuneka iphinde iqwalaselwe ukuze ukwazi ukuthatha usetyenziso olutsha kunye nokuhamba komsebenzi njengoko iimfuno ziguquka.Ngenkqubo eguquguqukayo, yeemodyuli, izinto ezininzi zokuhamba komsebenzi wakho wangoku zinokuphinda zisetyenziswe kwaye ziphuculwe.
Ngaba sikhona isisombululo esiphuma kwishelufu esihlangabezana neemfuno zakho?
Ezinye iindawo zokusebenzela ezikhethekileyo zenzelwe usetyenziso oluthile oluneprothokholi eziqinisekisiweyo, ezinje ngokutsalwa kweDNA, ukulungiswa kwesampulu, kunye nenkcubeko yeseli.Oku kunokwenza lula inkqubo yakho yokukhetha, kwaye kubonelele ngecandelo "elingundoqo" eliluncedo lokudityaniswa kwinkqubo enkulu kwixesha elizayo.Izisombululo ze-off-the-shelf ezenzelwe ukuhlanganiswa kwexesha elizayo kunye nokuguquguquka kwengqondo zikhethwa ukuba zingaguquki, iiplatifomu "ezivaliweyo".
Ingakanani indawo onayo, kwaye uyisebenzisa ngokufanelekileyo?
Indawo idla ngokuba yinto exabisekileyo.Uninzi lweenkqubo zokuphatha ulwelo ngoku zingabasebenzisi abaninzi, nto leyo eye yandisa imfuno yokuguquguquka kunye nokusetyenziswa okutsha kwendawo.Cinga ngokukhetha iqonga elizenzekelayo elinokufikelela kwindawo engaphantsi kwetafile yokusebenza ukufikelela kuyo, umzekelo, uhlalutyo olongezelelweyo okanye izixhobo zokulungiselela isampula, njl.
Kulula kangakanani ukugcina kunye nenkonzo?
Musa ukukubetha ngoyaba isevisi kunye nokugcinwa.Ukufikelela ngokulula ngamagcisa kunokunciphisa ixesha lokuphumla kunye nokuphazamiseka kokuhamba komsebenzi wakho.
Ukukhetha i-hardware efanelekileyo
Nokuba usebenza kwi-genomics, i-cell biology, ukufunyanwa kweziyobisi, ukuxilongwa kweemolekyuli, okanye into eyahlukileyo ngokupheleleyo, inkqubo yokuphatha ulwelo oluchanekileyo inokwenza ubomi bakho bube lula kakhulu.Iingqwalasela ezibalulekileyo ziquka:
Umbhobho wokushenxiswa komoya okanye ulwelo?
Ukufuduswa komoya kulungele ukuhambisa kuluhlu lwevolumu enkulu, ukusuka kwi-0.5 ukuya kwi-1,000 μL.Nangona ihambelana kuphela neengcebiso ezilahlayo, oku kwandisa isantya kunye nemveliso ngokususa amanyathelo angaphezulu ahambelana nepayipi yokususa ulwelo xa utshintsha ulwelo okanye ugungxulwa inkqubo.Kwakhona kunciphisa umngcipheko wokungcola kunye nokubonelela ngendlela ekhuselekileyo yokusingatha izinto ezinobungozi be-radioactive okanye biohazardous.
Ukufuduswa kolwelo kuyahambelana neengcebiso ezisisigxina nezilahlwayo, kwaye bubuchwephesha obukhethwayo bomthamo ogqithisiweyo ongaphantsi kwe-5 μL.Iingcebiso zetsimbi ezihlambulukileyo ezihlambekayo zifanelekile kwizicelo apho iibhubhu kufuneka zihlatywe okanye i-pipetting yoxinzelelo olufanelekileyo iyadingeka.Ukuguquguquka okuphezulu, qwalasela inkqubo ebandakanya ukufuduswa komoya kunye nolwelo.
Yeyiphi imiqulu kunye neefomathi osebenza ngazo?
Qinisekisa ukuba iqonga linokusingatha imibhobho yomthamo oyimfuneko kunye neefomathi zelebhu (iityhubhu kunye neepleyiti) ezisetyenziswa ngokuqhelekileyo kwilebhu yakho.Kwakhona qwalasela ukuba i-automation iya kuvumela isampulu encinci kunye ne-reagent volumes ukuba isetyenziswe, inika ugcino lweendleko ezinokubakho.
Zeziphi iingalo zemibhobho omele ukhethe?
Iindidi eziphambili ziyi-1) i-pipettes ye-channel eguquguqukayo-ngokuqhelekileyo i-1 ukuya kwi-8-channel-ekwazi ukuphatha iibhubhu, iiplate, kunye nezinye iifomathi ezininzi ze-labware;kunye ne-2) iingalo ezininzi ze-multichannel ezilungiselelwe ngokukodwa ukukhupha kwiipleyiti ezinamaqula amaninzi.Iinkqubo zale mihla zivumela iintloko zemibhobho okanye iipleyiti zeadaptha ukuba zitshintshwe “ngokubhabha”—ukhetho lobulumko kwiiprothokholi ezisebenzisa izincedisi ezininzi ezahlukahlukeneyo, njengenaliti ezingaguqukiyo, amacebiso alahlwayo, izixhobo zokukhonkxa ezinomthamo omncinci, njl.
Ngaba ufuna iingalo zerobhothingenxaukuba bhetyebhetye okongeziweyo?
Iingalo zokubamba zerobhothi zibonelela ngokuguquguquka okuphezulu ngokuhambisa ilebhu ejikeleze idekhi yomsebenzi.Iingalo zerobhothi ezinokutshintsha "iminwe" yazo ngokukhawuleza ziqinisekisa ukuguquguquka okukhulu kunye nokubamba okukhuselekileyo kuzo zombini iibhubhu kunye neepleyiti.
Loluphi uhlobo lwengcebiso yepipette oluya kwandisa ukuveliswa kwakhona?
Umgangatho wengcebiso ngumnikeli ophambili wokuphindaphinda kwaye unokwenza okanye ukwaphule ukusebenza kwenkqubo.Iingcebiso ezilahlwayo zihlala zibonwa njengolona khetho lulungileyo lokuphelisa ungcoliseko phakathi kweesampulu zebhayoloji.Abanye abathengisi ngoku banikezela ngeengcebiso ezikhethekileyo ezinomthamo ophantsi oqinisekisiweyo wokuhanjiswa okuthembekileyo kumanqanaba e-microliter okanye i-submicroliter efunekayo kwizicelo ezinjenge-assay miniaturization.Cinga ngokuthenga ibhrendi yomthengisi we-automation yeengcebiso zepipette ukuqinisekisa ukuba ufumana ezona ziphumo zithembekileyo.
Izixhobo ezisebenzisa iingcebiso ezisisigxina zinokuba neenzuzo ngokubhekiselele kwiindleko zokusebenza.Iinaliti zentsimbi ezizinzileyo zihlala zifikelela ezantsi kweenqanawa ezinzulu ngakumbi kuneengcebiso ezilahlwayo, kwaye zinokugqobhoza i-septa.Izikhululo zokuhlambela eziyilwe ngokufanelekileyo zinciphisa umngcipheko wokungcoliseka ngokunqamlezayo ngolu cwangciso.
Ngaba ufuna iingcebiso eziqinisekisiweyo ukuba zinyumba?
Ukunciphisa umngcipheko wosulelo, sebenzisa kuphela izinto ezityiwayo ezibhalwe “ziyinyumba.”Ezi zenziwa phantsi kweemeko ezingqongqo kwaye zihambelane nemigangatho yokupakishwa kunye neyothutho eqinisekisa ubunyumba kuyo yonke indlela eya kwibhentshi yelebhu.Iimveliso ezibhalwe "presterile" azinyumba xa zishiya umenzi, kodwa zidibana namathuba amaninzi ongcoliseko kamva.
Isoftware ibalulekile
I-Software ibonelela nge-interface kunye nomntu omiselayo kunye nokusebenza kwesixhobo, kwaye ukuyila kwayo kuya kugqiba ukuba kulula kangakanani ukucwangcisa kunye nokusebenzisana nenkqubo ukuqwalasela ukuhamba komsebenzi, ukuseta iiparameters zenkqubo, kunye nokwenza ukhetho lokuphatha idatha.Ikwanalo negalelo elithe ngqo malunga nokuba lungakanani na uqeqesho olufunekayo ukuze kuqhutywe inkqubo ngokuzithemba.Ngaphandle kokuba une-software technician endlini, isoftware eyilwe kakubi, nokuba inamandla kangakanani na, inokukushiya uxhomekeke kumthengisi okanye ingcali yangaphandle ukuphuhlisa iiprothokholi ezilungiselelweyo, iingxaki zokusombulula iingxaki, kwaye wenze nolona tshintsho lulula lwenkqubo.Kwiilebhu ezininzi, umqhubi wenkqubo akayiyo ingcali yokucwangcisa, kwaye uninzi lwamaqela e-IT aluyi kubandakanyeka ngokuthe ngqo kwisoftware yokulawula isixhobo.Ngenxa yoko, kungafuneka ulinde ukuba kubekho abacebisi bangaphandle, nto leyo ephazamisa kakhulu imveliso kwaye ibeka amaxesha eprojekthi emngciphekweni.
Amanqaku okuqwalasela
Imibuzo ephambili ekufuneka uyibuze xa uvavanya isoftware yokuphathwa kwenkqubo yolwelo ibandakanya:
- Ngaba abaqhubi banokunxibelelana nesikrini sokuchukumisa ukuze usebenze yonke imihla?
- Ngaba umthengisi unalo ithala leencwadi lemigaqo ekhoyo yokwenza lula inkqubo?
- Ngawaphi amandla okudityaniswa kwesoftware yezixhobo zomntu wesithathu?
- Ingakanani ilayibrari yomqhubi wesixhobo ebonelelwa ngumthengisi?
- Ngaba umthengisi unamava ngokudibana kwe-LIMS?
- Ungakhululeka ukwenza inkqubo ngokwakho?
- Kulula kangakanani kubasebenzisi ukuseta ii-run zabo ngaphandle kobuchule benkqubo?
- Zeziphi izinto—ezifana nezikhokelo zokulayisha umzobo ozenzekelayo—ozifunayo, kwaye ngaba ziyafumaneka?
- Ngaba kulula ukuphinda uqwalasele isoftware xa inkqubo iphinda ijongwe?
- Ngaba umthengisi anganceda ukuqinisekisa ukhuseleko lwe-cyber?
Isampulu yokulandeleka
Ukulandelelwa kwesampulu epheleleyo kunokubaluleka ekuthotyelweni kwemigangatho yomgangatho kunye nezikhokelo.Ukuleyibhelishwa kwebhakhowudi, kunye nesoftware efanelekileyo, kuya kwenza lula ukulandelela iisampuli kunye nezinto ezisetyenziswayo, kwaye kunokuthintela ukulahleka kokulandeleka.Ukuleyibhela okuzenzekelayo kunye nezisombululo zokulandela umkhondo nazo zinoku:
- Bonisa indawo yelebhu kumgangatho kunye nakwiiyunithi zokugcina
- Qinisekisa ukuba iilebhile zebhakhowudi zisetyenziswe ngokufanelekileyo kwaye zinokufundwa ngokuchanekileyo
- Ukukhawulezisa ukufundwa kwebhakhowudi kunye neenkqubo zokuchola iisampulu, kwaye ulungelelanise ukudityaniswa kwe-middleware kunye ne-LIMS.
Inketho yokungenelela
Iimpazamo zenziwe ngokulula, kodwa akusoloko kulula ukuzilungisa.Iisistim ezininzi ezizisebenzelayo azinayo imisebenzi "yokuqala / yokumisa" okanye "hlehlisa", nto leyo enokuthetha ukuqalisa kwakhona inkqubo ukuba ufaka into engalunganga okanye ufuna ukunqumamisa inkqubo.Khangela inkqubo ye-automation ehlakaniphile ekwazi ukubona, ukuqonda, ukunika ingxelo, kunye nokubuyisela kwimpazamo, ngokuqala / ukuyeka ukusebenza ukuvumela ukusebenzisana okukhuselekileyo kunye nokulula kwabaqhubi kunye nommandla womsebenzi wesixhobo ngexesha lokuqhuba.
Isishwankathelo
Ukuphatha ulwelo oluzenzekelayo kunokuphelisa imisebenzi emininzi edinayo, ukuphucula imveliso kunye nokukhulula ixesha elixabisekileyo lomsebenzi obaluleke ngakumbi-kodwa kuphela ukuba uphumeza izisombululo ezifanelekileyo.Ukuziqwalasela ngenyameko iingongoma ekuxutyushwe ngazo kweli nqaku kuya kunceda iilabhoratri zikhethe ngobulumko, nto leyo ezenza zikwazi ukufumana ingenelo yokuphatha ulwelo oluzisebenzelayo zize zenze ubomi bube lula yaye bunemveliso.
Ixesha lokuposa: May-10-2022