Amapuleti emithombo ejulile awuhlobo lwemishini yaselabhorethri esetshenziswa kusiko lamaseli, ukuhlaziya amakhemikhali ezinto eziphilayo, nezinye izinhlelo zokusebenza zesayensi.Aklanyelwe ukubamba amasampula amaningi emithonjeni ehlukene, okuvumela abacwaningi ukuthi benze izivivinyo ngesilinganiso esikhulu kunezitsha ze-petri ezivamile noma amashubhu okuhlola.
Izingcwecwe zemithombo ejulile ziza ngobukhulu obuhlukahlukene kanye nokuma, kusukela ku-6 kuya ku-96 imithombo.Okuvame kakhulu amapuleti anemithombo engu-96, anomumo ongunxande futhi athatha imithombo yesampula ngayinye emigqeni engu-8 ngamakholomu angu-12.Umthamo wevolumu womthombo ngamunye uyahlukahluka kuye ngobukhulu bawo, kodwa ngokuvamile uphakathi kuka-0.1 mL - 2 mL umthombo.Amapuleti emithombo ejulile abuye abe nezivalo ezisiza ukuvikela amasampula ekungcoleni ngesikhathi sokugcinwa noma ukuthutha futhi anikeze isivalo esingangenisi umoya lapho efakwe ku-incubator noma shaker ngesikhathi sokuhlolwa.
Izingcwecwe zemithombo ejulile zinemisebenzi eminingi embonini yesayensi yempilo;zivame ukusetshenziswa esikweni lamangqamuzana, njengezifundo zokukhula kwebhaktheriya, ukuhlolwa kwe-cloning, amasu okukhipha i-DNA/ama-amplification afana ne-PCR (i-polymerase chain reaction) ne-ELISA (i-enzyme-linked immunosorbent assay) .Ngaphezu kwalokho, amapuleti emithombo ejulile angasetshenziselwa izifundo ze-enzyme kinetic, ukuhlolwa kokuhlolwa kwe-antibody, kanye namaphrojekthi okucwaninga okutholakala kwezidakamizwa, phakathi kokunye.
Amapuleti angama-96-well-well-well anikeza inzuzo enkulu ngaphezu kwamanye amafomethi njengoba ekhulisa indawo engaphezulu kumthamo wevolumu - uma kuqhathaniswa namafomethi amancane afana namapuleti angama-24- noma angu-48, lokhu kuvumela amaseli amaningi noma ama-molecule ukuthi acutshungulwe ngesikhathi esisodwa. ngenkathi usaqhubeka Gcina amazinga okulungiswa anele ngokuhlukana kwamadiski.Ukwengeza, lezi zinhlobo zamapuleti zenza ososayensi bakwazi ukwenza ngokuzenzakalelayo izinqubo besebenzisa izinhlelo ze-robotic, bakhulise kakhulu amandla okukhipha ngaphandle kokuyekethisa amazinga okunemba;into engenzeki kusetshenziswa izindlela zendabuko ezifana ne-pipetting manual.
Kafushane, kusobala ukuthi kungani izingcwecwe zemithombo ejulile engu-96 zisetshenziswa kabanzi ezindaweni eziningi ezihlukene zocwaningo lwesayensi;ngenxa yobukhulu befomethi yabo enkulu, bavumela abacwaningi ukuguquguquka okukhulu ekwenzeni izivivinyo ngenkathi benikeza isikhathi sokucubungula esisebenzayo, okwenza kube kuhle kumalabhorethri anamuhla emhlabeni jikelele!
Isikhathi sokuthumela: Feb-23-2023