96 noi papa luawai hohonu

ʻO nā papa luawai hohonu kahi ʻano mea hana keʻena hana i hoʻohana ʻia i ka moʻomeheu cell, biochemical analysis, a me nā noi ʻepekema ʻē aʻe.Hoʻolālā ʻia lākou e hoʻopaʻa i nā laʻana he nui i loko o nā pūnāwai kaʻawale, e ʻae ana i nā mea noiʻi e hana i nā hoʻokolohua ma kahi ʻoi aku ka nui ma mua o nā kīʻaha petri kuʻuna a i ʻole nā ​​​​paipu hoʻāʻo.

Hiki mai nā papa punawai hohonu i nā ʻano nui a me nā ʻano like ʻole, mai ka 6 a hiki i ka 96 luawai.ʻO ka mea maʻamau, ʻo ia nā papa 96-well, he ʻāpana ʻehā i ke ʻano a hoʻokomo i nā pūnāwai hoʻohālike pākahi ma 8 lālani me 12 kolamu.ʻOkoʻa ka mana volumetric o kēlā me kēia luawai e like me kona nui, akā ma waena o 0.1 mL - 2 mL no ka pūnāwai.Hele pū mai nā papa luawai hohonu me nā poʻi e kōkua i ka pale ʻana i nā laʻana mai ka hoʻohaumia ʻana i ka wā o ka mālama ʻana a i ʻole ka lawe ʻana a hāʻawi i kahi sila paʻa i ka ea ke hoʻokomo ʻia i loko o ka incubator a i ʻole ka lulu i ka wā o nā hoʻokolohua.

Nui nā hoʻohana ʻana o nā pā puna wai hohonu i ka ʻoihana ʻepekema ola;Hoʻohana pinepine ʻia lākou i ka moʻomeheu cell, e like me nā haʻawina ulu bacteria, nā hoʻokolohua cloning, DNA extraction/amplification techniques like PCR (polymerase chain reaction) a me ELISA (enzyme-linked immunosorbent assay).Eia kekahi, hiki ke hoʻohana ʻia nā papa hohonu hohonu no nā haʻawina kinetic enzyme, nā hoʻāʻo screening antibody, a me nā papahana noiʻi ʻike lāʻau, a me nā mea ʻē aʻe.

Hāʻawi nā papa punawai hohonu 96 i kahi pōmaikaʻi nui ma mua o nā ʻano ʻē aʻe i ko lākou hoʻonui ʻana i ka ʻili o ka ʻili i ka ratio leo - hoʻohālikelike ʻia i nā ʻano liʻiliʻi e like me nā pā 24- a i ʻole 48-well, ʻae kēia i nā cell a i ʻole nā ​​molekole e hana ʻia i ka manawa hoʻokahi. ʻoiai e mālama mau i nā pae hoʻonā kūpono no nā disks.Eia hou, hiki i kēia mau ʻano papa ke hiki i nā ʻepekema ke hoʻokaʻawale i nā kaʻina hana me ka hoʻohana ʻana i nā ʻōnaehana robotic, me ka hoʻonui nui ʻana i nā mana throughput me ka ʻole o ka hoʻohālikelike ʻana i nā pae pololei;ʻAʻole hiki ke hoʻohana i nā hana kuʻuna e like me ka pipetting manual.

I ka hōʻuluʻuluʻana, maopopo ke kumu i hoʻohana nuiʻia ai nā papa 96-deep-well ma nā wahi likeʻole o ka noiʻiʻepekema;ma muli o ka nui o ko lākou ʻano nui, ʻae lākou i ka poʻe noiʻi i ʻoi aku ka maʻalahi i ka hana ʻana i nā hoʻokolohua me ka hāʻawi ʻana i ka manawa hoʻoponopono kūpono, e kūpono ana i nā laboratories hou a puni ka honua!