Nigute ushobora gufunga icyapa cya PCR

Intangiriro

Ibyapa bya PCR, ikirangantego cya laboratoire imyaka myinshi, kiragenda kigaragara cyane mugihe kigezweho kuko laboratoire yagura ibicuruzwa byayo kandi igakoresha gukoresha automatike mubikorwa byabo.Kugera kuri izi ntego mugihe kubungabunga ukuri nubunyangamugayo byubushakashatsi birashobora kugorana.Kimwe mu bice bisanzwe aho amakosa ashobora kunyerera ni hamwe na kashe yaIbyapa bya PCR, hamwe na tekinike mbi yemerera guhumeka byintangarugero, guhindura pH bityo bigahagarika imikorere yimisemburo, no gutumira umwanda.Kwiga gushiraho ikimenyetso aIcyapa cya PCRikuraho neza izo ngaruka kandi ikemeza ibisubizo byororoka.

Shakisha Ikimenyetso Cyiza cya plaque yawe

Isahani yerekana isahani hamwe na kashe ya firime
Ingoferoninzira nziza yo gufunga isahani yawe hamwe na kashe ifunze, mugihe ukomeje kuguha guhinduka kugirango byoroshye guhisha no gukuraho isahani nkuko ubikeneye nta myanda.Ariko, ingofero zifite ibice bibiri byingenzi.

Ubwa mbere, ugomba kugura capa yihariye ihuje, ituma idahinduka.Uzakenera kwemeza neza ko agapira wahisemo gahuye nisahani, iterwa nuwagikoze, hanyuma ugahitamo domed cyangwa igorofa ukurikije thermocycler ukoresha.

Icya kabiri, gushira ingofero ku isahani birashobora gusubiramo cyane kandi bikarambirana, hamwe ningaruka zo kwanduzanya uramutse ushyize ingofero itari nziza neza.

Mugihe kashe ya firime idahinduka muburyo bwo gukuraho no kuyisimbuza, irahuza cyane kuko izahuza ubwoko ubwo aribwo bwose bwa plaque ya PCR, hatitawe kubayikora.Birashobora gukatirwa gusa mubunini, bigatuma bikora neza.

Ubundi buryo ni igipfundikizo cy'isahani.Ibi bitanga uburinzi buke bufata kashe na kashe, kandi bikoreshwa gusa mugihe gito kugirango wirinde kwanduza.

Ikirangantego cyiza na kashe ya firime

Niba ukeneye optique, kashe isobanutse cyangwa anfirime ya aluminiumgufunga isahani yawe byemejwe nuburyo bwawe bwo kugerageza.Amafirime mezaBiragaragara kugirango wemererwe kwitegereza ingero, mugihe ukirinda kandi ukirinda guhumeka.Zifite kandi akamaro kanini mubushakashatsi bwa qPCR zirimo gukora ibipimo nyabyo bya fluorescence biturutse ku isahani, icyo gihe uzakenera firime ifunga kayunguruzo ya fluorescence nkeya ishoboka.Ni ngombwa kwemeza neza ko kashe cyangwa capa ukoresha ifite urwego rwo hejuru ruhagije rwo guhitamo neza kugirango ibyasomwe neza.

Filime ya file irakwiriye kuburugero urwo arirwo rwose rworoshye cyangwa rugomba kubikwa munsi ya 80 ° C.Kubera iyo mpamvu, ibyitegererezo byinshi bigenewe kubikwa igihe kirekire bizakenera firime.Filime zidafite ishingiro nazo ziracumita, zifite akamaro ko gusuzuma amariba kugiti cye, cyangwa guhererekanya ingero n'inshinge.Ibi birashobora kubaho intoki cyangwa nkigice cya robot.

Tekereza kandi ko ibintu bikaze birimo acide, ibishingwe cyangwa umusemburo bizakenera kashe ishobora kubyihanganira, mugihe ikidodo gishobora kuba gikwiye.

Amashanyarazi hamwe na firime yo gushyushya
Ikirangantego cya firimeni Byoroheje-Imbere kandi byoroshye gusaba.Icyo ukeneye ni uwukoresha kugirango ashyireho kashe ku isahani, kandi ukoreshe igikoresho cyoroshye cyo gusaba kugirango ukande hasi hanyuma ushireho kashe ikomeye.

Ikidodo gishushe kirateye imbere, gitanga ikidodo kirekire kirekire cyagabanije igipimo cyuka ugereranije nikimenyetso gisanzwe gifatika.Ihitamo rirakwiriye niba ushaka kubika ibyitegererezo mugihe kirekire, nubwo ibi biza hamwe nibindi bisabwa kubikoresho bifunga amasahani.

Nigute ushobora gufunga icyapa cya PCR

Uburyo bwo Gufunga Isahani

Kwifata wenyine

1. Menya neza ko urimo ukora kumurongo uringaniye kandi uhamye

2. Kuraho firime mubipfunyika, hanyuma ukure inyuma

3. Witonze ushireho kashe ku isahani, mugihe urebe neza ko amariba yose yatwikiriwe

4. Koresha igikoresho cyo gusaba kugirango ushire igitutu hejuru yisahani.Tangirira kumpera imwe hanyuma ukore inzira yawe ujya kurundi, ukande neza

5. Subiramo ibi inshuro nyinshi

6. Koresha uwagusabye kuzenguruka amariba yo hanze, kugirango umenye neza ko nayo afunze neza.

Ubushyuhe bwa kashe

Ubushyuhe bwa kashe bukora mugushonga firime kumurongo wa buri riba, hifashishijwe kashe ya plaque.Kugira ngo ushireho ubushyuhe, reba amabwiriza yatanzwe nuwakoze ibikoresho.Menya neza ko uwagukoreye inkomoko y'ibikoresho byawe azwi, kuko ari ngombwa cyane ko kashe ikwiye, ikora neza kandi ikagira amazi.

Isahani yo gufunga inama

a.Mugihe ushyizeho igitutu kuri kashe, jya mubyerekezo bitambitse kandi bihagaritse kugirango umenye neza kashe

b.Burigihe nibyiza gukora ikizamini cyo gukora ibyo ukora byose, kandi ibi ntaho bitandukaniye no gufunga amasahani.Gerageza ufite isahani irimo ubusa mbere yo gukoresha imwe ifite ingero.

c.Mugihe cyo kwipimisha, kura kashe hanyuma urebe ko ibifatika byamanutse neza, nta cyuho.Hano haribigaragara byerekana ibi mubisobanuro byambere.Niba utarafunze neza isahani neza, mugihe ukuyeho kashe hazabaho icyuho aho ibifatika bitigeze bihuza neza nisahani.

d.Kubijyanye no kohereza no gutwara ingero, ushobora gusanga ari byiza gushyira kashe ya plastike hejuru yikimenyetso cya file kugirango ukingire (cyane cyane gutobora).

e.Buri gihe ujye umenya neza ko nta bisebe cyangwa iminkanyari iyo ukoresheje firime - ibi bizatera kumeneka no guhumeka