Tips, as consumables used with pipettes, generally can be divided into standard tips; filtered tips; conductive filter pipette tips, etc.
1. The standard tip is a widely used tip. Almost all pipetting operations can use ordinary tips, which are the most affordable type of tips.
2. The filtered tip is a consumable designed to avoid cross-contamination and is often used in experiments such as molecular biology, cytology, and virology.
3. The surface of the low-adsorption tip has undergone a hydrophobic treatment, which can reduce the low surface tension liquid leaving more residues in the tip.
PS: The wide-mouth tip is ideal for sucking viscous materials, genomic DNA, and cell culture fluid.
How to choose a good pipette tip?
The statement can be said to be partly true but not entirely true. The tip that can be mounted on the pipette can indeed form a pipetting system with the pipette to realize the pipetting function, but is this reliable? A question mark is required here.
Tip features of the pipette tip
So what are the minimum points that a good tip must-have?
A good tip depends on concentricity, taper, and the most important point is adsorption;
1. Let’s talk about the taper first: if it is better, the match with the pipette is very good.
2. Concentricity: Concentricity is whether the circle between the tip of the tip and the link between the tip and the pipette is the same center. If it is not the same center, it means that the concentricity is not good;
3. Finally, the most important one is our absorptivity: absorptivity is related to the material of the tip. If the material of the tip is not good, it will affect the accuracy of pipetting and cause a large amount of liquid to be retained or referred to as hanging on the wall, causing errors in pipetting.
So everyone should pay special attention to the above three points when choosing a pipette tip. A row of bad tips is clearly spaced differently! You will see obvious distortions, but this is a very important step in choosing a good tip. Also, please note the installation of the tips on the single-channel pipette and the multi-channel pipette is different. For the single-channel one, insert the tip vertically into the pipette tip, press lightly, and turn it slightly to tighten it. For multi-channel, the multiple channels of the pipette should be aligned with multiple tips, inserted at an angle, and slightly shake back and forth to tighten; Do not repeatedly hit the pipette to ensure the airtightness of the tip.
Answering this question requires data to speak
1. Match the pipette with the tip for performance testing.
2. Calculate the accuracy of the pipetting operation after converting it into volume according to the density of the test liquid.
3. What we have to choose is to have a good tip. If the pipette and the tip are not well-matched, it means that the tightness of the tip and the pipette cannot be guaranteed, making the results of each operation impossible to reproduce.
Post time: Dec-10-2022