Pono nā ʻano wai paipu kūikawā

ʻoki ʻoe i kapiko paipui ka paipu ana i ka glycerol?Ua hana wau i kaʻu PhD, akā pono wau e aʻo e hoʻonui kēia i ka pololei a me ka pololei ʻole o kaʻu paipu.A ʻo ka ʻoiaʻiʻo i ka wā e ʻoki ai au i ka piko, hiki iaʻu ke ninini pololei i ka glycerol mai ka ʻōmole i loko o ka paipu.No laila ua hoʻololi au i kaʻu ʻenehana no ka hoʻomaikaʻi ʻana i nā hopena pipetting a loaʻa i nā hopena hilinaʻi a hiki ke hana hou ʻia i ka wā e hana ai me nā wai viscous.

ʻO kahi ʻano wai e pono ai ka nānā pono ʻana i ka wā o ka paipu ʻana he wai viscous.Hoʻohana pinepine ʻia kēia mau mea i loko o ka lab, a i ʻole ma ke ʻano maʻemaʻe a i ʻole he ʻāpana buffer.ʻO nā ʻelele kaulana o nā wai viscous i loko o nā keʻena noiʻi he glycerol, Triton X-100 a me Tween® 20. Akā, ʻo nā laboratories e hana ana i ka mana maikaʻi o nā meaʻai, nā mea hoʻonaninani, nā lāʻau lapaʻau a me nā huahana mea kūʻai aku e pili ana i nā hopena viscous i kēlā me kēia lā.

Hōʻike ʻia ka viscosity ma ke ʻano he dynamic, a i ʻole kinematic viscosity.Ma kēia ʻatikala, hoʻopaʻa wau i ka viscosity dynamic o nā wai no ka mea e wehewehe ana i ka neʻe ʻana o ka wai.Hōʻike ʻia ka degere o ka viscosity i millipascal i kekona (mPa*s).Hiki ke hoʻololi ʻia nā laʻana wai ma kahi o 200 mPa*s e like me 85 % glycerol me ka hoʻohana ʻana i ka pipette air-cushion maʻamau.I ka hoʻohana ʻana i kahi ʻenehana kūikawā, hoʻihoʻi ʻia ka paipu ʻana, ka manaʻo o nā ʻōpū o ka ea a i ʻole nā ​​koena i ka piko e hoʻemi nui ʻia a alakaʻi i nā hopena pipetting pololei.Akā naʻe, ʻaʻole ʻo ia ka mea maikaʻi loa e hiki ai iā mākou ke hana no ka hoʻomaikaʻi ʻana i ka pipetting o nā wai viscous (e nānā i ka fig. 1).

Ke piʻi ka viscosity, piʻi ka pilikia.ʻOi aku ka paʻakikī o ka hoʻoneʻe ʻia ʻana o nā haʻina haʻahaʻa haʻahaʻa a hiki i 1,000 mPa*s me ka hoʻohana ʻana i nā pipette air-cushion maʻamau.Ma muli o ke kiʻekiʻe o ka hoʻoheheʻe ʻana i loko o nā molekole, he lohi ka holo ʻana o ka wai viscous a pono e hana mālie a akahele ka paipu.ʻAʻole lawa ka ʻenehana Reverse Pipetting no ka hoʻoili wai pololei ʻana a nui ka poʻe e kaupaona i kā lākou mau laʻana.ʻO kēia hoʻolālā ke ʻano o ka lawe ʻana i ka nui o ka wai a me nā kūlana ʻoihana e like me ka haʻahaʻa a me ka mahana e helu pono ai i ka nui o ka wai e pono ai i ke kaumaha.No laila, ʻōlelo ʻia nā mea hana pipetting ʻē aʻe, i kapa ʻia nā mea hana hoʻoneʻe maikaʻi.Loaʻa iā lākou ka piko me kahi piston i hoʻohui ʻia, e like me ka syringe.No laila, hiki ke maʻalahi a hoʻokaʻawale ʻia ka wai i ka wā e hāʻawi ʻia ai ka hoʻoili wai kūpono.ʻAʻole pono kahi ʻenehana kūikawā.

Eia nō naʻe, hiki i nā mea hana hoʻoneʻe maikaʻi i ka palena me nā hopena viscous e like me ka meli wai, ka ʻili ʻili a i ʻole kekahi mau aila mechanical.Pono kēia mau wai koʻikoʻi ʻē aʻe e hoʻohana ana i ka loina hoʻoneʻe maikaʻi akā loaʻa kahi hoʻolālā i hoʻolālā ʻia e hoʻoponopono i nā hopena viscous loa.Ua hoʻohālikelike ʻia kēia mea hana kūikawā me nā ʻōlelo aʻoaʻo hoʻoneʻe maikaʻi e loaʻa i kahi paepae kahi e pono ai ke hoʻololi mai kahi ʻāpana hoʻopuka maʻamau i kahi kikoʻī kūikawā no nā hopena viscous loa.Ua hōʻike ʻia ua hoʻonui ʻia ka pololei a ua hoʻemi ʻia nā ikaika e pono ai no ka aspiration a me ka hāʻawi ʻana i ka wā e hoʻohana ai i kahi kikoʻī kūikawā no nā wai viscous loa.No ka ʻike kikoʻī a me nā hiʻohiʻona wai, e ʻoluʻolu e hoʻoiho i ka Applicaton Note 376 ma ka hana i hoʻopaʻa ʻia no nā wai liʻiliʻi loa.