Ukuqala isilingo kusho ukubuza imibuzo eminingi.Iyiphi impahla edingekayo?Imaphi amasampula asetshenziswayo?Yiziphi izimo ezidingekayo, isb, ukukhula?Lude kangakanani uhlelo lonke?Ingabe kufanele ngihlole ukuhlolwa ngezimpelasonto, noma ebusuku?Umbuzo owodwa uvame ukukhohlwa, kodwa awubalulekile kangako.Yiziphi uketshezi ezisetshenziswa ngesikhathi sokufaka isicelo futhi zifakwa kanjani ngamapayipi?
Njengoba uketshezi lwamapayipi kuwumsebenzi wansuku zonke futhi uma i-liquid aspirated nayo ikhishwa, ngokuvamile asichithi isikhathi esiningi nomzamo kulesi sihloko.Kodwa kunengqondo ukucabanga kabili mayelana nethuluzi le-liquid ne-pipette elisetshenzisiwe.
Uketshezi lungahlukaniswa ngezigaba ezinhlanu eziyinhloko: ezinamanzi, i-viscous (kuhlanganise nezinto zokuhlanza), eziguquguqukayo, eziminyene futhi ezithathelwanayo noma ezinobuthi.Ukungaphathwa ngendlela efanele kwalezi zigaba zoketshezi kunethonya elikhulu kumphumela wepayipi.Ngenkathi izixazululo ezinamapayipi ezinamapayipi njengama-buffer amaningi kulula futhi kwenziwa ikakhulukazi ngamapayipi e-air-cushion ajwayelekile, kungase kuphakame ubunzima lapho kufakwe amapayipi uketshezi olushintshashintshayo njenge-acetone.Uketshezi oluguquguqukayo lunomfutho womoya ophezulu obangela ukuhwamuka ku-air-cushion kanjalo nokwakheka kwamaconsi.Ekugcineni, lokhu kusho ukulahleka kwesampula noma i-reagent ngaphandle kwenqubo efanele yepayipi.Lapho pipetting uketshezi oluguquguqukayo, pre-wetting of theithiphu ye-pipette(ukulangazelela okuphindaphindiwe kanye nemijikelezo yokukhipha ukuze kuthuliswe umoya ngaphakathi kwethiphu) kuyimpoqo ukuze kwandiswe ukunemba kwamapayipi.Isigaba samanzi esihluke ngokuphelele sihlanganisa uketshezi lwe-viscous njenge-glycerol.Lezi zinokugeleza okunensa kakhulu ngenxa yokungqubuzana okuphezulu kwangaphakathi kwama-molecule okuholela ekuphupheni kwamabhamuza omoya, izinsalela zethiphu kanye nesampula noma ukulahleka kwe-reagent.Indlela ekhethekile yokufaka amapayipi ebizwa ngokuthi i-reverse pipetting iyatuswa uma usebenzisa ama-pipette e-air-cushion yakudala.Kodwa okungcono kakhulu ukusetshenziswa kwethuluzi elihlukile lamapayipi, ithuluzi lokususa elivumayo elinephuzu elifana nesirinji elisebenza ngaphandle komcamelo womoya phakathi kwesampula nepiston ngaphakathi kwethiphu.Uketshezi lungashiswa ngokushesha nakalula ngalawa mathuluzi.Lapho ukhipha uketshezi lwe-viscous, umthamo ophelele ungakhishwa ngaphandle kwezinsalela ku-tip.
Ngakho-ke, ukucabanga ngoketshezi ngaphambi kokuqala ukuhlola kungenza kube lula futhi kuthuthukise ukuhamba kwakho komsebenzi nemiphumela.Uhlolojikelele lwezigaba zoketshezi, izinselele zazo kanye nezincomo mayelana namasu afanelekile okufaka amapayipi kanye namathuluzi okufaka amapayipi kuboniswa kuphosta yethu.Ungalanda iphosta ukuze ube nenguqulo ephrintekayo ngelebhu yakho.
Inkampani yeSuzhou ACE Biomedical Technology Co., Ltd., Ltd yinkampani yobungcweti ezibophezele ekuhlinzekeni ngezinto ezisetshenziswayo zekhwalithi ephezulu zezokwelapha nezelebhu ezisetshenziswa ezibhedlela, emitholampilo, kumalebhu okuxilonga kanye namalebhu ocwaningo lwesayensi yezempilo.Sinebanga leamathiphu we-pipette (Amathiphu ajwayelekile, amathiphu azenzakalelayo), i-microplate(24,48,96 imithombo), I-PCR Consumables (PCR plate, amashubhu, amafilimu okuthengisa),I-Cryovial Tubenokunye, singahlinzeka ngesevisi ye-OEM/ODM, wamukelekile ukuthintana nathi uma unezidingo.
I-Suzhou ACE Biomedical Technology Co., Ltd
I-imeyili:Joeyren@ace-biomedical.com
Ucingo:+86 18912386807
Iwebhusayithi:www.ace-biomedical.com
Isikhathi sokuthumela: Feb-09-2023