Ukuhlanza i-AutoclavePipette Amathiphukubalulekile ekugcineni ukuphepha kwelebhu kanye nokuqinisekisa imiphumela enembile. Amathiphu angewona oyinyumba angethula ukungcoliswa kwamagciwane, okuholela emaphutheni nasekubambezelekeni kokuhlolwa. I-Autoclaving iphumelela kakhulu, iqeda amagciwane afana nesikhunta namagciwane. Uma kuqhathaniswa nezinye izindlela, inikeza ubunyumba obuphelele, okuyenza ibaluleke emikhubeni ethembekile yaselabhorethri.
Ukulungiselela Amathiphu e-Autoclaving Pipette
Izinto Ezidingekayo Zoku-Autoclaving
Ukuze wenze inzalo ngokuphepha amathiphu e-pipette, udinga izinto ezifanele. Njalo sebenzisa amathiphu e-pipette enziwe nge-polypropylene noma ama-copolymers ayo, njengoba lezi zinto zingakwazi ukumelana ne-autoclaving ephindaphindiwe. Gwema ukusebenzisa amathiphu e-polyethylene, njengoba angase ancibilike ngaphansi kwamazinga okushisa aphezulu. Qinisekisa ukuthi amathiphu abhalwe ukuthi "Autoclavable" ukuze uqinisekise ukufaneleka kwawo. Ukwengeza, uzodinga ama-racks aphephile e-autoclave noma amacala okubulala inzalo ukuze ubambe amathiphu phakathi nenqubo. Lawa ma-rack asiza ukugcina ubuqotho bamathiphu futhi aqinisekise ukujikeleza komoya okufanele ukuze kubulawe inzalo ephumelelayo.
Ukuhlola Amathiphu e-Pipette Ngokulimala noma Ukungcola
Ngaphambi kwe-autoclaving, hlola ithiphu ngalinye le-pipette ukuze uthole imifantu, ama-chips, noma omunye umonakalo obonakalayo. Amathiphu alimele angafaka engozini ubunyumba futhi aholele emiphumeleni engalungile. Hlola noma yikuphi ukungcola okuyinsalela, okufana noketshezi olomisiwe noma izinhlayiya, ezingaphazamisa inqubo yokuvala inzalo. Lahla noma yimaphi amathiphu abonisa izimpawu zomonakalo noma ukungcola ukuze ugcine ubuqotho bokuhlolwa kwakho.
Ukuhlanza Amathiphu E-Pipette Asetshenzisiwe Ngaphambi Kokwenza I-Autoclaving
Uma usebenzisa kabusha amathiphu e-pipette, ahlanze kahle ngaphambi kokwenza i-autoclaving. Hlanza amathiphu ngamanzi acwecwe ukuze ususe noma yiziphi izinsalela zamakhemikhali. Ukuze uthole ukungcola okunenkani, sebenzisa isixazululo esibulalayo ukuze uqinisekise ukususwa okuphelele. Ukuhlanza ngendlela efanele akugcini nje ngokuthuthukisa ubunyumba kodwa futhi kuvimbela izinsalela ukuthi zingathinti ukusebenza kwe-autoclave.
Ilayisha Amathiphu e-Pipette ku-Autoclave-Safe Racks
Beka amathiphu e-pipette kuma-racks aphephile e-autoclave noma amakesi okubulala inzalo. Zihlele ngendlela evumela ukuhamba kahle komoya. Gwema ukulayisha ngokweqile ama-rack, ngoba lokhu kungaphazamisa inqubo yokuvala inzalo. Uma usebenzisa amathiphu oyinyumba avaliwe, ungawavezi ngokuzenzakalelayo futhi, njengoba aseyinyumba. Uma sekulayishiwe, qiniseka ukuthi ama-racks abekwe ngokuphephile ukuze avimbele ukuthinta ngesikhathi somjikelezo we-autoclaving.
Ukulungiselela Amathiphu e-Autoclaving Pipette
Ukusetha i-Autoclave
Ngaphambi kokuqala, qiniseka ukuthi i-autoclave ihlanzekile futhi isebenza kahle. Hlola inqolobane yamanzi bese uyigcwalisa uma kunesidingo. Hlola i-gasket yomnyango ukuze uthole noma yiziphi izimpawu zokuguga noma ukulimala, njengoba lokhu kungase kuphazamise inqubo. Hlala ulandela imiyalelo yomkhiqizi ukuze umise i-autoclave ngendlela efanele. Ukusebenzisa i-autoclave enakekelwe kahle kuqinisekisa ubunyumba bamathiphu akho e-pipette futhi kuvimbela ukungcoliswa okuphambene.
Ukukhetha Umjikelezo Olungile Wokusterilisa
Ukukhetha umjikelezo ofanele kubalulekile ekubulaleni okusebenzayo. Imijikelezo evamile ihlanganisa:
- Umjikelezo wamandla adonsela phansi: Ithembele ekugelezeni kwesitimu semvelo futhi ilungele amathiphu e-pipette. Ibeke ku-252 ° F imizuzu engu-20 kubha eyodwa yokucindezela okuhlobene.
- Vacuum (prevac) umjikelezo: Isebenzisa ivacuum ukukhipha umoya ngaphambi kokwethula isitimu, iqinisekisa ukungena okungcono.
- Umjikelezo woketshezi: Idizayinelwe iziqukathi ezigcwele uketshezi kodwa ngokuvamile ayisetshenziselwa amathiphu e-pipette.
Ukukhetha amathiphu e-pipette angakwazi ukumelana nalezi zimo kubalulekile ukuze kugcinwe ubuqotho babo.
Ilayisha i-Autoclave Ngokuphephile
Lapho ulayisha i-autoclave, gqoka okokuvikela umuntu siqu (i-PPE) njengamagilavu, izibuko zokuphepha, nejazi laselabhu. Hlela ama-racks ngesikhala esanele phakathi kwawo ukuze uvumele ukujikeleza kwe-steam. Gwema ukupakisha ngokuqinile i-autoclave, njengoba lokhu kungavimbela ukuvala inzalo. Qinisekisa ukuthi izivalo zamathreyi athiphu zivuleke kancane ukuvumela isitimu singene. Ungalokothi ugoqe izinto nge-foil, njengoba ibamba umswakama futhi ivimbele ukuvala inzalo ngendlela efanele.
Isebenzisa i-Autoclave kanye nokuqapha Inqubo
Qala i-autoclave futhi uqaphe inqubo eduze. Hlola izinga lokushisa, ukucindezela, nesikhathi somjikelezo ukuze uqinisekise ukuthi zihlangabezana nezilungiselelo ezidingekayo. Sebenzisa izinkomba zamakhemikhali zangaphakathi, ezifana nohlobo 4 noma uhlobo lwesi-5, ukuze uqinisekise ukuthi izinto ezibulala amagciwane zingenile ephaketheni. Ukuqapha komshini, njengokubheka amageji, kusiza ukuqinisekisa ukuthi i-autoclave isebenza ngendlela efanele. Bhala inqubo yokulandeleka nokuqinisekisa ikhwalithi.
Ukupholisa nokukhipha i-Autoclave
Uma umjikelezo usuqedile, vumela i-autoclave ukuthi iphole ngaphambi kokuyivula. Hlola igeji yokucindezela ukuze uqinisekise ukuthi ifundeka 0 PSI. Yima ngemva komnyango futhi uwuvule kancane ukuze ukhulule insalela yesitimu ngokuphepha. Vumela amathiphu e-pipette aphole ngokwemvelo ngaphakathi kwe-autoclave ukuze ugcine ubunyumba. Ukuze omiswe ngokushesha, dlulisela ama-rack kukhabhinethi yokomisa esethwe ku-55°C. Ukupholisa okufanele nokwehliswa kwempahla kuvimbela ukulimala kwamathiphu ekhwalithi ephezulu futhi kulondoloze ukusebenza kwawo.
Ukusetshenziswa Kwethiphu Ye-Pipette Ye-Post-Autoclaving kanye Nesitoreji
Ukususa Amathiphu E-Pipette Enziwe Inzalo Ngokuphepha
Ukuphatha amathiphu e-pipette afakwe inzalo ngendlela efanele kubalulekile ekugcineni ubunyumba bawo. Gqoka amagilavu ngaso sonke isikhathi ukuze uvimbele ukungcoliswa kokuthintwa kwesikhumba. Sebenzisa kuphela izinto ezidlekayo ezilebulwe ngokuthi "okuyinyumba" ukuze unciphise ubungozi. Ngaphambi kokusebenzisa amathiphu, hlanza i-pipette kanye nomnikazi wayo nge-ethanol engu-70%. Lesi sinyathelo siqinisekisa ukuthi akukho ukungcola okulimaza ubunyumba bamathiphu. Uma ususa amathiphu ku-autoclave, gwema ukuwaveza ukuze uvule umoya isikhathi eside. Zidlulisele ngqo esitsheni esihlanzekile, esivalekile noma emaphaketheni azo asekuqaleni ukuze ulondoloze ubuqotho bawo.
Amathiphu Okuhlola Okulimala Kwangemva Kokwenza Inzalo
Ngemuva kwe-autoclaving, hlola amathiphu we-pipette nganoma yiziphi izimpawu zomonakalo. Bheka ukungqubuzana, ukuqhekeka, noma ukushintsha umbala, njengoba lezi zinkinga zingathinta ukusebenza kwazo. Amathiphu alimele angase afake engozini ukunemba kokuhlolwa kwakho noma ethule ukungcola. Lahla noma yimaphi amathiphu abonisa amaphutha abonakalayo. Lesi sinyathelo sokuhlola siqinisekisa ukuthi amathiphu aphezulu kuphela, angenalutho asetshenziswa emsebenzini wakho.
Ukugcina Amathiphu E-Pipette Ukugcina Inzalo
Ukugcinwa okufanele kubalulekile ukugcina amathiphu e-pipette eyinyumba ngemva kwe-autoclaving. Gcina amathiphu emaphaketheni awo angempela avaliwe noma esitsheni esingangeni moya ukuze uvimbele ukuchayeka kukungcola. Gwema ukugoqa amabhokisi amathiphu ku-foil, njengoba lokhu kungabamba umswakama futhi kukhuthaze ukukhula kwama-microbial. Beka isitsha sokugcina endaweni epholile, eyomile ekude nelanga eliqondile kanye nomswakama. Hlanza amabhokisi okugcina njalo ukuze ulondoloze ukusebenza kahle kwawo. Lezi zinqubo zisiza ukuqinisekisa ubunyumba bamathiphu akho e-pipette kuze kube yilapho esetshenziswa ngokulandelayo.
Ukulebula Nokuhlela Amathiphu Afakwe Inzalo
Ukulebula nokuhlela amathiphu akho e-pipette asterilized kuthuthukisa ukusebenza kahle futhi kunciphisa amaphutha. Sebenzisa amalebula acacile ukuze ubonise idethi yokuvala inzalo kanye nohlobo lwamathiphu agciniwe. Hlela amathiphu ngosayizi noma ngohlelo lokusebenza ukuze uwenze kube lula ukuwathola ngesikhathi sokuhlolwa. Gcina indawo yokugcina ihlanzekile ukuze ugweme ukungcoliswa ngephutha. Ukuhleleka ngendlela efanele akugcini nje ngokugcina isikhathi kodwa futhi kuqinisekisa ukuthi uhlale unamathiphu oyinyumba alungele ukusetshenziswa.
Amaphutha Avamile Lapho U-Autoclaving Pipette Amathiphu
Ilayisha ngokweqile i-Autoclave
Ukulayisha ngokweqile i-autoclave kubeka engcupheni inqubo yokuvala inzalo. Uma upakisha amathiphu amaningi e-pipette ekamelweni, umusi awukwazi ukujikeleza ngokuphumelelayo. Lokhu kubangela ukuvala inzalo ngokungalingani, okushiya amanye amathiphu engenanyumba. Hlala uhlela amathiphu kuma-autoclave-safe racks anesikhala esanele phakathi kwawo. Gwema ukupakisha ama-racks aqine kakhulu. Isikhala esifanele siqinisekisa ukuthi isitimu sifinyelela kuwo wonke amathiphu, sigcina ubunyumba nobuqotho babo.
Ukusebenzisa izilungiselelo ze-Autoclave ezingalungile
Izilungiselelo ezingalungile zingalimaza amathiphu e-pipette noma zehluleke ukuwavala inzalo. Isibonelo, amathiphu e-pipette kufanele afakwe i-autoclaved kanye kuphela ku-121 ° C imizuzu engu-10, kulandelwa umjikelezo wokumisa ku-110 ° C imizuzu engu-5. Ukusebenzisa amazinga okushisa aphakeme noma imijikelezo emide kungenza amathiphu abe brittle noma kubangele ukuthi izihlungi zibhanqe. Izingozi ezihlobene nezilungiselelo ezingalungile zihlanganisa:
| Ubungozi bokuphepha | Incazelo |
|---|---|
| Ukushisa kuyasha | Kusuka ezintweni ezishisayo nasezindongeni zegumbi le-autoclave nomnyango |
| I-steam iyasha | Kusuka ku-steam eyinsalela ekhishwe ngemva komjikelezo |
| Uketshezi olushisayo olushisayo | Kusuka oketshezini olubilisayo noma okuchithekile ngaphakathi kwe-autoclave |
| Ukulimala kwezandla nengalo | Lapho uvala umnyango we-autoclave |
| Ukulimala komzimba | Uma kukhona ukuqhuma ngenxa yengcindezi engafanele noma ukulayisha |
Hlala ulandela imihlahlandlela yomkhiqizi ukuze ukhethe umjikelezo olungile wamathiphu we-autoclave pipette.
Ukweqa Izinyathelo Zokuhlanza Ngaphambili
Ukweqa izinyathelo zokuhlanza ngaphambilini kuholela ezinkingeni zokungcola. Amakhemikhali ayizinsalela noma izinto zebhayoloji kumathiphu asetshenzisiwe angaphazamisa ukuvala inzalo. Lokhu kungaholela kokuthi:
- Ukungcola kwe-Pipette-to-sampuli, lapho i-pipette yethula ukungcola kusampula.
- Ukungcoliswa kwesampula kuya kupayipi, lapho isampula lingcolisa umzimba we-pipette.
- Ukungcoliswa kwesampula kuya kwesampula, lapho izinsalela zidluliswa phakathi kwamasampuli.
Hlanza kahle amathiphu ngamanzi a-distilled noma isisombululo se-chemical decontamination ngaphambi kwe-autoclaving. Lesi sinyathelo sibalulekile ekuvimbeleni ukungcoliswa okuphambene nokuqinisekisa imiphumela ethembekile.
Ukuphatha Okungafanele Ngemva Kokuvalwa Kwenzalo
Ukuphatha amathiphu okuvalwa inzalo ngendlela engafanele kungahlehlisa inqubo yokuvala inzalo. Gqoka amagilavu njalo lapho ukhipha amathiphu ku-autoclave. Gwema ukuthinta amathiphu ngokuqondile noma ukuwaveza ukuze uvule umoya isikhathi eside. Zidlulisele ngokushesha ezitsheni ezivaliwe noma ama-racks aklanyelwe ukusetshenziswa nokugcinwa kwethiphu ye-pipette. Lezi zinqubo zisiza ukugcina ubunyumba bamathiphu akho.
Amathiphu Okugcina Ezimeni Ezingezona Inyumba
Ukugcina amathiphu ezimeni ezingezona inyumba kuwaveza kokungcola. Sebenzisa iziqukathi ezingangeni moya noma amabhokisi amathiphu avaliwe ukuze uvikele amathiphu angenalutho. Gwema amathiphu okugoqa ku-foil, njengoba ibamba umswakama futhi ikhuthaze ukukhula kwama-microbial. Gcina amathiphu endaweni epholile, eyomile ukuze ulondoloze ubunyumba kanye nokuphikiswa kwamakhemikhali kwamathiphu e-pipette. Isitoreji esifanele siqinisekisa ubuqotho bamathiphu akho ukuze uwasebenzise esikhathini esizayo.
Ithiphu: Njalo hlola amathiphu ngomonakalo noma i-waping ngemva kwe-autoclaving. Amathiphu alimele angafaka engozini ukuhlola kwakho futhi aholele emiphumeleni engalungile.
Amathiphu okufaka inzalo nge-pipette abalulekile ekugcineni ukuphepha kwelebhu nokuqinisekisa imiphumela enembile. Ukuvala inzalo ngendlela efanele kuvimbela ukungcola, kugcina ubuqotho bokuhlolwa kwakho, futhi kusekela imiphumela ethembekile.
Ukufingqa, landela lezi zinyathelo zokuvala inzalo ngempumelelo:
- Lungiselela ngokuhlola nokuhlanza amathiphu e-pipette.
- I-Autoclave isebenzisa izilungiselelo ezifanele futhi uqinisekise ukuhamba komoya okufanele.
- Ngemuva kokuvala inzalo, phatha amathiphu ngokucophelela futhi uwagcine ezitsheni ezivaliwe ukuze ugcine ubunyumba.
Izinto ezibalulekile ezithathwayo zokuphepha kwelebhu zihlanganisa:
- Sebenzisa ama-autoclave ukuze uqede ukwakheka kwama-microbial.
- Gcina amathiphu emaphaketheni awo oqobo noma ezitsheni ezingangeni moya.
- Hlola amathiphu ngomonakalo ngaphambi kokuwasebenzisa futhi ugweme ukuwaveza ukuze uvule umoya.
Ngokulandela lezi zinqubo, uqinisekisa ukugcinwa nokusetshenziswa kwamathiphu e-pipette oyinyumba, anciphisa izingozi zokungcola futhi athuthukise ukunemba kokuhlolwa.
Isikhathi sokuthumela: Feb-19-2025