Iinkqubo zokuphatha ulwelo oluzenzekelayo zineengenelo ezininzi xa uphethe ulwelo oluyingxaki njengolwelo lwe-viscous okanye oluguquguqukayo, kunye nemithamo emincinci kakhulu.Iinkqubo zinezicwangciso zokuhambisa iziphumo ezichanekileyo nezithembekileyo kunye namaqhinga athile anokucwangciswa kwisoftware.
Ekuqaleni, inkqubo yokuphatha ulwelo oluzenzekelayo inokubonakala inzima kwaye iyoyisa.Kodwa xa sele uqalile ukusebenza ngezi zixhobo, uya kuqaphela ukuba zikwenza lula ukuhamba kwakho.Iinjineli ziphuhlise izinto ezininzi ezahlukeneyo ukuququzelela ucelomngeni lwezicelo.
Xa uphethe imiqulu emincinci ngeenkqubo zokuphatha ulwelo oluzenzekelayo, kuyenzeka ukuba unqwenele zonke ii-reagents ezifunekayo kwimpendulo kwenye.ingcebiso, yahlulwe ngumsantsa womoya.Ubuchwephesha buxoxwa ngokubanzi, ngakumbi malunga nokungcoliseka kolwelo olwahlukeneyo ngamathontsi angaphandleincam yepipette.Abanye abavelisi bacebisa oku nangona kunjalo ukugcina ixesha kunye nomzamo.Iinkqubo zinokufuna amanzi kuqala, zilandelwa yi-reagent A, emva koko i-reagent B, njl. njl. Umaleko ngamnye wolwelo wahlulwe ngesikhewu somoya ukuthintela ukuxubana okanye ukusabela kuqale ngaphakathi kwincam.Xa ulwelo lukhutshwa, zonke ii-reagents zixutywe ngokuthe ngqo kwaye imiqulu encinci ihlanjwe ngaphandleingcebisongemithamo emikhulu kwincam.Inqaku kufuneka litshintshwe emva kwesinyathelo ngasinye sepayipi.
Ukhetho olungcono kukusebenzisa izixhobo ezikhethekileyo ezilungiselelwe iivolumu ezincinci, umz, ugqithiselo lwemithamo ye-1 µL kwijethi yasimahla.Oku kwandisa isantya kwaye kuthintela ukungcoliseka okunqamlezayo.Ukuba imiqulu engaphantsi kwe-1 µl ifakwe imibhobho, kungcono ukulahla ngokuthe ngqo kulwelo ekujoliswe kulo okanye ngokuchasene nomphezulu wenqanawa ukukhupha umthamo wonke.Ukunikezela ngemithamo emincinci ngokudibana kolwelo kuyacetyiswa xa ulwelo olucela umngeni olufana nolwelo lwe-viscous lufakwe ngemibhobho.
Olunye uphawu oluluncedo kakhulu lweenkqubo zokuphatha ulwelo oluzenzekelayo kukudipha ngencam.Xa kuphela 1 µL isampulu inqwenelwa kwiingcebiso, ithontsi engamanzi ihlala incamathela ngaphandle kweingcebisongexesha lokukhulelwa.Kunokwenzeka ukucwangcisa i-tip ukuntywila kulwelo equleni ukuze i-drops kunye ne-micro-drops kumphezulu wangaphandle we-tip ifikelele kwi-reaction.
Ngaphaya koko, ukuseta umnqweno kunye nesantya sokuhambisa kunye nevolumu yokuphuma kunye nesantya kuyanceda.Isantya esifanelekileyo sohlobo ngalunye lolwelo kunye nomthamo unokucwangciswa.Kwaye ukuseta ezi parameters kukhokelela kwiziphumo eziphindaphindekayo kakhulu ngenxa yokuba i-pipette ngesantya esahlukileyo yonke imihla kuxhomekeke ekusebenzeni kwethu.Ukuphatha ulwelo oluzenzekelayo kunokuphucula ingqondo yakho kwaye kwandise ukuthembela kwizicelo ezinomngeni ngokuthatha iindawo ezicaphukisayo.
Ixesha lokuposa: Feb-07-2023