E ka 'na eaba micropipette ke sesebelisoa se sebelisoang ka ho fetisisa laboratoring.Li sebelisoa ke bo-ramahlale mafapheng a fapaneng a fapaneng ho kenyeletsoa academia, sepetlele le forensics labs hammoho le nts'etsopele ea lithethefatsi le ente ho fetisetsa mokelikeli o nepahetseng, o fokolang haholo.
Le hoja e ka ba ntho e tenang le e ferekanyang ho bona lipululoana tsa moea karolong e lahloang ea pipette haeba li sa bonoa kapa li sa hlokomolohuoe, ho ka ba le tšusumetso e kholo ho tšepahala le ho ikatisa ha liphetho.
Litaba tse monate ke hore ho na le mehato e bonolo eo u ka e nkang ho thibela li-bubble tsa moea, le ho ntlafatsa ts'ebetso ea lab, khotsofalo ea basebelisi hammoho le ho nepahala le ho nepahala ha liphetho.
Ka tlase, re hlahloba sephetho sa ho fumana bubble moeeng ntlheng ea hau ea pipette le seo u lokelang ho se etsa kamora moo.
Liphello tsa Bubbles kaKeletso ea Pipette
Esita le haeba u sebelisa li-pipette tse nepahetseng ka ho fetisisa, tse ka holimo-limo, tse hlokometsoeng hantle, tse sebeletsoang le tse lekantsoeng, ho tšepahala ha liphetho tsa hau ho ka angoa ke liphoso tsa lab.Ha li-bubble li kena ka har'akeletsoe ka ba le liphetho tse 'maloa.
● Ha mosebelisi a bona bubble ea moea o tlameha ho qeta nako a ntse a fana ka mokelikeli o phophomang ka nepo, ntša ntlha ebe o qala tšebetso hape.
● Li-bubble tsa moea tse sa bonahaleng li ka fella ka ho fetisa molumo o tlaase, kahoo ho fetola motsoako oa metsoako e lebisang litekong tse hlōlehileng le liphello tse belaetsang kapa tse sa tšepahaleng.
Liphetho tsena li ka ba le litlamorao tse 'maloa (1).
● Ho Fokotsa Matla a Lab - Liteko le litlhahlobo li tla tlameha ho phetoa, ho tlisa litšenyehelo tsa basebetsi le thepa, tse ka bang ngata haholo.
● Liphetho tse belaetsang kapa tse sa nepahalang - Haeba liphetho tse fosahetseng li lokolloa ho ka ba le liphello tse tebileng ho feta tse kenyeletsang tlhahlobo e fosahetseng le liphello tse mpe tsa mokuli.
● Ho Hlakola Lingoliloeng ho Tsoa Lingoliloeng - Haeba lithaka tsa hau li hloleha ho hlahisa liphetho tsa hau ka lebaka la li-bubble tsa moea tse bakang liphetho tse sa nepahalang lipampiri li ka tlosoa.
Mekhoa e Molemo ka ho Fetisisa ea ho Thibela Lipulumo Tsa Moea
Maemong a mangata li-bubble tsa moea ka malebela a pipette li bakoa ke phoso ea opareitara.Mokhoa o fosahetseng ka lebaka la koetliso e sa lekaneng kapa mokhathala hangata ke bothata bo ka sehloohong.
Pipetting ke ts'ebetso e nang le boiphihlelo e hlokang tlhokomelo ea 110%, koetliso e nepahetseng le boikoetliso ho fihlela liphetho tse tsitsitseng le tse nepahetseng.
Le ha ho na le lintho tse 'maloa tseo u ka li etsang ho fokotsa liphoso tse akaretsang tsa lipeipi, ka tlase re hlakisitse mekhoa e meng e metle e ka sebelisoang ho qoba li-bubble tsa moea.malebela a pipette.
Ntlafatsa Mokhoa oa Basebelisi
Pipette Butle-butle
Haeba plunger e lokolloa kapele haholo ha e lakatsa, libubble tsa moea li ka kenngoa ntlheng.Sena se ka ba bothata haholo-holo ha ho fetisoa lino tse nang le viscous.Phello e tšoanang e ka etsahala haeba plunger e lokolloa kapele ka mor'a ho fana.
E le ho qoba li-bubble tsa moea ha u lakatsa, ela hloko ho sebelisa piston ea pipette ea letsoho ka mokhoa o boreleli le o tloaelehileng, ho sebelisa matla a tsitsitseng.
Sebelisa Botebo bo Nepahetseng ba ho Koalloa
Ho hloleha ho qoelisa ntlha ea pipette botebong bo lekaneng ka tlas'a meniscus ea letamo la metsi ho ka fella ka takatso ea moea 'me kahoo ho thehoa ha bubble.
Leha ho le joalo, ho qoelisa ntlha e tebileng haholo ho ka lakatsa metsi a mangata ka lebaka la khatello e eketsehileng kapa marotholi a ka hlaha ka ntle ho ntlha, kahoo ho bohlokoa ho qoelisa.ntlha ea pipettebotebong bo nepahetseng.
Botebo bo khothalletsoang bo fapana pakeng tsa boholo ba pipette, mofuta le ho etsa.Leha likhothaletso tsa moetsi li lokela ho lateloa mona ke tataiso e akaretsang e fanoeng ke National Physical Laboratory.
Tataiso ho Botebo ba Tip Tip
Bophahamo ba Pipette (µl) & Botebo ba ho Ikamela (mm)
- 1 - 100: 2 - 3
- 100 - 1,000: 2 - 4
- 1,000 - 5,000: 2 - 5
Metsi a peleLitlhahiso tsa Pipette
Ha liphaephe li phahama ho feta 10µlmalebela a pipettehangata li kolobisoa esale pele ka ho li tlatsa ka makhetlo a 'maloa ka mokelikeli o fanoang le ho o ntša ho senya ho ntlafatsa ho nepahala.
Ho hloleha ho li kolobisa pele ho ka baka lipululana tsa moea, haholo-holo ha u sebelisa li-viscous kapa li-hydrophobic liquid.Ho qoba libubble tsa moea, etsa bonnete ba hore o na le malebela a pele a metsi ha molumo oa liphala o feta 10µl.
Sebelisa Mekhoa ea ho Ruta Liphaephe ka Morao ha e Nepahetse
Viscous Substances: Bothata bo tloaelehileng ha li-pipetting viscous substances tse kang protheine kapa nucleic acids solutions, glycerol le Tween 20/40/60/80 ke ho thehoa khafetsa ha li-bubble ha ho sebelisoa mokhoa oa pele oa pipetting.
Ho kenya liphaephe butle-butle, ho sebelisa mokhoa oa ho khutlisa liphaephe ho fokotsa kotsi ea ho thehoa ha bubble ha ho fetisoa litharollo tsa viscous.
ELISA Technik
Reverse pipetting e boetse e khothaletsoa ha ho kenya liphaephe tse nyane ho kena96 lipoleiti tsa liteko tse nyane tse nyanebakeng sa mekhoa ea ELISA.Ha li-bubble tsa moea li huleloa ka har'a pipette kapa li kenngoa ka lilibeng ha li eketsa li-reagents li ka susumetsa litekanyetso tsa optical density le liphello.Reverse pipetting e khothalletsoa ho fokotsa kapa ho felisa taba ena.
Sebelisa Ergonomic Pipettes
Li-pipette tsa setaele sa khale tse sa qaptjoang ho nahanoa ka ergonomics li hloka boikitlaetso bo eketsehileng ba 'mele, oa khathala 'me mokhoa oa hau oa ho roala lipeipi o fetoha bohlasoa le ho fokola.Liphoso tse boletsoeng ka holimo tse kang ho lokolloa ha plunger kapele li ka hlaha hangata.
Ka ho tsetela ka tharollo ea ergonomic ho feta, u tla khona ho boloka mokhoa o motle haholo le ho thibela sebopeho sa li-bubble tsa moea ka lebaka la mokhoa o fosahetseng.
Iphe Nako ea ho Koetlisa Basebetsi
Ho koetlisa le ho hlahloba basebetsi khafetsa ka mekhoa ea liphaephe ho ka etsa bonnete ba hore phoso ea mokhanni le sebopeho sa bubble ea moea li fokotsehile.
Nahana ka Litharollo Tse Ling tse Iketselitsoeng
Joalokaha ho boletsoe ka holimo, li-bubble tse ngata tsa moea li bakoa ke opareitara.Ho ka khonahala ho fokotsa phoso le matšeliso a opareitara ka ho sebelisa li-pipette tsa elektroniki kapa sethala se bonolo sa ho sebetsana le mokelikeli joalo kaAgilent Bravo Liquid Ho sebetsana le Robot.
Sebelisa Boleng bo MolemoLitlhahiso tsa Pipette
Hangata li-micropipettes li rekoa ka tlhokomelo, empa hangata ho nahanoa hanyane ka boleng ba ntlha ea pipette e lahliloeng.Ka lebaka la tšusumetso eo ntlha e nang le eona liphethong tsa liphaephe, standardISO 8655 e hloka tlhahlobo e eketsehileng haeba ho sebelisoa lipeipi le malebela a tsoang ho bahlahisi ba fapaneng.
Sena se ka bakoa ke hore litlhahiso tse ngata tse theko e tlaase li ka shebahala li le ntle qalong empa ha u li ithuta ka hloko li ka 'na tsa e-ba le mahlaseli, li-protrusions, mengoapo le lipudula tsa moea, kapa tsa kobeha kapa tsa ba le litšila.
Ho reka malebela a boleng bo botle a entsoeng ka polypropylene ea boemo bo holimo ho ka fokotsa ho hlaha ha li-bubble tsa moea.
Ho Qetella
Ho fumana li-bubble tsa moea ntlheng ea hau ea pipette ho na le tšusumetso ho sebetsa hantle ha lab hammoho le ho se nepahale le ho se nepahale ha liphetho.Re hlokometse lintho tse 'maloa tseo u ka li etsang ho qoba hore li-bubble tsa moea li kenellentlha ea pipette.
Leha ho le joalo, haeba boleng bo bobemalebela a pipetteli etsa hore li-bubble tsa moea li kene ntlheng ea hau ea pipette, u tla thabela ho tseba hore bokahohle ba rona bo lekanamalebela a pipetteli entsoe ka litekanyetso tse phahameng ka ho fetisisa 'me li entsoe ka polypropylene e hloekileng ea premium-grade.
Khamphani ea Suzhou Ace Biomedicalhlahisa boleng ba boleng bo holimo ba 10,20,50,100,200,300,1000 le 1250 μL malebela a pipette a bokahohleng, malebela a 96/rack.Ho tšoarella ho ikhethang - li-racks tsohle tsa ACE li lumellana le litlhoko tsa tšebeliso le li-pipettor tse ngata.E nyopa, Sefe, RNase-/DNase-free, le nonpyrogenic.
Rea u amohela ho re botsa bakeng sa lintlha tse ling.
Nako ea poso: Dec-29-2022