Micropipette birashoboka ko igikoresho gikoreshwa cyane muri laboratoire.Bakoreshwa nabahanga mubice bitandukanye birimo za kaminuza, ibitaro na laboratoire ya laboratoire hamwe no guteza imbere ibiyobyabwenge ninkingo kugirango bimure neza, bike cyane byamazi
Mugihe bishobora kutubabaza no gutesha umutwe kubona imyuka ihumeka mumashanyarazi ya disipuline iyo itabonetse cyangwa yirengagijwe irashobora kugira ingaruka nini kubwizerwa no kubyara ibisubizo.
Amakuru meza nuko hari ingamba zoroshye ushobora gufata kugirango wirinde guhumeka ikirere, no kunoza imikorere ya laboratoire, kunyurwa kwabakozi kimwe nukuri kandi neza kubisubizo.
Hasi, turasesengura ingaruka zo kubona umwuka mubi mumpanuro yawe hamwe nibyo ugomba gukora ubutaha.
Ingaruka za Bubbles muriImpanuro
Nubwo wakoresha neza cyane, hejuru yurwego, ukomezwa neza, ukorerwa hamwe na kalibibasi ya pipeti kwizerwa ryibisubizo byawe birashobora guterwa namakosa ya laboratoire.Iyo ibituba byinjiye muriinamairashobora kugira ibisubizo byinshi.
● Mugihe umukoresha ashyizeho umwuka mwinshi bagomba kumara umwanya wo gutanga amazi yifuzwa uko bikwiye, kura inama hanyuma utangire inzira.
Air Ibibyimba bitamenyekanye byumuyaga bishobora kuvamo amajwi make, bityo ugahindura imitekerereze ya reaction ivanze biganisha kubigeragezo byananiranye nibisubizo bikemangwa cyangwa bitizewe.
Ibisubizo birashobora kugira ingaruka nyinshi (1).
Kugabanuka kwa Laboratwari - Ibizamini hamwe nibisubizo bizagomba gusubirwamo, bitwara abakozi nibiciro, bishobora kuba byinshi.
Results Ibisubizo by'ibizamini bikemangwa cyangwa bitari byo - Niba ibisubizo bitari byo byashyizwe ahagaragara hashobora kubaho ingaruka zikomeye zirimo kwisuzumisha nabi hamwe n’ibisubizo by’abarwayi.
Gukuramo inyandiko zandikishijwe intoki ziva mu binyamakuru - Niba urungano rwananiwe kwigana ibisubizo byawe kubera umwuka mwinshi utera ibisubizo bidahwitse impapuro zishobora gukurwaho.
Imyitozo myiza yo gukumira umwuka mubi
Mubenshi mubibazo byumwuka mubyifuzo bya pipette biterwa nikosa ryabakoresha.Tekinike mbi kubera imyitozo idahagije cyangwa umunaniro mubisanzwe nikibazo cyibanze.
Pipetting nigikorwa cyubuhanga gisaba kwitabwaho 110%, imyitozo ikwiye nimyitozo kugirango ugere kubisubizo bihamye kandi byukuri.
Mugihe hari ibintu byinshi ushobora gukora kugirango ugabanye amakosa rusange yo gutembera, hepfo twerekanye imikorere myiza ishobora gukoreshwa kugirango wirinde umwuka mubiinama.
Kunoza uburyo bwabakoresha
Pipette Buhoro
Niba plunger irekuwe vuba mugihe wifuza, umwuka mwinshi urashobora kwinjizwa mumutwe.Ibi birashobora kuba ikibazo cyane mugihe cyohereza amazi ya viscous.Ingaruka nkiyi irashobora kubaho mugihe plunger irekuwe vuba nyuma yo gutanga.
Kugira ngo wirinde guhumeka ikirere mugihe wifuza, witondere gukoresha piston yimiyoboro yintoki muburyo bworoshye kandi busanzwe, ukoreshe imbaraga zihamye.
Koresha Ubujyakuzimu Bwuzuye
Kunanirwa kwibiza umuyoboro wa pipette byimbitse bihagije munsi ya menisque yikigega cyamazi gishobora kuvamo icyifuzo cyumwuka bityo bigatuma habaho ububobere.
Ariko, kwibiza hejuru cyane birashobora kwifuza amazi menshi kubera umuvuduko mwinshi cyangwa ibitonyanga bishobora kugaragara hanze yisonga bityo rero ni ngombwa kwibiza uumuyoboroKuri ubujyakuzimu.
Ubujyakuzimu busabwa buratandukanye hagati yubunini bwa pipette, ubwoko no gukora.Mugihe ibyifuzo byabakora bigomba gukurikizwa dore ubuyobozi rusange butangwa na Laboratwari yigihugu.
Imiyoboro Yimbitse Yibiza
Umubumbe wa Pipette (µl) & Ubujyakuzimu (mm)
- 1 - 100: 2 - 3
- 100 - 1.000: 2 - 4
- 1.000 - 5.000: 2 - 5
ImbereInama
Iyo imiyoboro irenze 10µlinamamubisanzwe babanje kubanza kubuzuza inshuro nyinshi hamwe namazi yatanzwe hanyuma bakayirukana imyanda kugirango banoze neza.
Kunanirwa kubanza kubitonyanga birashobora kuvamo umwuka mwinshi, cyane cyane iyo ukoresheje amazi ya viscous cyangwa hydrophobique.Kugirango wirinde ibyuka bihumeka neza mbere yuko utose mugihe imiyoboro irenze 10µl.
Koresha uburyo bwo guhinduranya imiyoboro ihanitse niba bikwiye
Ibintu Viscous: Ikibazo gikunze kugaragara mugihe cyo gutobora ibintu byijimye nka proteine cyangwa nucleic acide ibisubizo, glycerol na Hagati ya 20/40/60/80 nuburyo bukunze kubaho ibibyimba iyo hakoreshejwe uburyo bwo kuvoma imbere.
Kuvoma gahoro gahoro, ukoresheje tekinoroji yo guhinduranya bigabanya ibyago byo kwibumbira mugihe cyohereza ibisubizo biboneye.
Ubuhanga bwa ELISA
Gusubira inyuma nabyo birasabwa mugihe cyo gutobora ingano nto96 neza ibyapa bipimakubuhanga bwa ELISA.Iyo umwuka mwinshi ushushe muri pipette cyangwa ugatangwa mumariba mugihe wongeyeho reagent birashobora kugira ingaruka nziza yubucucike nibisubizo.Guhindura imiyoboro isabwa kugabanya cyangwa gukuraho iki kibazo.
Koresha imiyoboro ya Ergonomic
Imiyoboro ishaje itarakozwe muburyo bwa ergonomique mubitekerezo bisaba imbaraga nyinshi zumubiri, urarushye kandi tekinike yawe yo kuvoma iba ibicucu kandi ikennye.Amakosa yavuzwe haruguru nko kurekura byihuse birashobora kugaragara cyane.
Mugushora mubisubizo byinshi bya ergonomic uzashobora gukomeza tekinike nziza kandi wirinde guhumeka ikirere kubera tekinike mbi.
Fata umwanya wo guhugura abakozi
Guhugura buri gihe no gusuzuma abakozi muburyo bwo gutobora imiyoboro irashobora kwemeza ko ikosa ryabakoresha no guhumeka ikirere bigabanuka.
Reba Ibisubizo Byikora Byikora
Nkuko byavuzwe haruguru ibyuka byinshi byo mu kirere biterwa nuwabikoze.Birashoboka kugabanya ikosa ryabakoresha no guhumurizwa ukoresheje imiyoboro ya elegitoronike cyangwa uburyo bworoshye bwo gukoresha amazi nkaAgilent Bravo Amazi Yimashini.
Koresha UbwizaInama
Micropipettes isanzwe igurwa ubyitondeye, ariko akenshi usanga ibitekerezo bike bihabwa ubwiza bwinama ya pipette ikoreshwa.Bitewe ningaruka inama ifite kubisubizo byo kuvoma, standardISO 8655 isaba kalibrasi yinyongera niba imiyoboro ninama zitangwa nababikora batandukanye.
Ibi birashobora kuba kubera ko inama nyinshi zihenze zirashobora kugaragara neza muburyo bwambere ariko iyo ubyize witonze zirashobora kugira flash, protrusions, scratches, hamwe nu mwuka mwinshi, cyangwa byunamye cyangwa birimo umwanda.
Kugura inama nziza zakozwe na polypropilene yo murwego rwohejuru irashobora kugabanya kubaho kwimyuka myinshi.
Kwanzura
Kubona umwuka mubi mumpanuro yawe ya pipette bigira ingaruka kumikorere ya laboratoire kimwe no kutamenya neza ibisubizo.Twabonye ibintu byinshi ushobora gukora kugirango wirinde umwuka mwinshi winjira muriumuyoboro.
Ariko, niba ubuziranenge bubiinamazitera umwuka mubi kwinjira mumpanuro yawe, uzanezezwa no kumenya ko isi yose ikwiyeinamabikozwe kurwego rwohejuru kandi bikozwe hamwe na premium-grade ya polypropilene.
Suzhou Ace Biomedicalkubyara umusaruro-mwiza 10,20,50,100,200,300.1000 na 1250 µL umubumbe wa pipeti rusange, inama 96 / rack.Kuramba bidasanzwe - ibisobanuro byose bya ACE bihagaze kubisabwa kugirango ukoreshe imiyoboro myinshi.Sterile, Akayunguruzo, RNase- / DNase-yubusa, na nonpyrogenic.
Murakaza neza kugirango mutubaze ibisobanuro birambuye.
Igihe cyo kohereza: Ukuboza-29-2022