Akatswiri a labotale amatha maola ambiri tsiku lililonse atanyamula kachipangizo kakang'ono, ndipo kuwongolera bwino kwa mapaipi ndikuwonetsetsa kuti zotsatira zodalirika nthawi zambiri zimakhala zovuta. sikuti zimangotsimikizira kuti kuyesera kulikonse, komanso kumawonjezera mphamvu.Kumvetsetsa zofunikira za kayendedwe ka pipetting kumathandiza ogwiritsa ntchito kusankha ma pipette olondola komanso obwerezabwereza, koma pali zinthu zina zambiri zomwe ziyenera kuganiziridwa kuti zithetse zotsatira za pipetting ndikutsimikizira kupambana kwa mayesero.
Kunena mwachidule, zamadzimadzi zimagwera m'magulu atatu akuluakulu: amadzimadzi, owoneka bwino, komanso osasunthika.Zamadzimadzi zambiri zimakhala zochokera m'madzi, zomwe zimapangitsa kuti ma pipette asamuke mpweya akhale chisankho choyamba kwa ambiri.Ngakhale kuti zakumwa zambiri zimagwira ntchito bwino ndi mtundu uwu wa pipette, ma pipette a volumetric ayenera kusankhidwa pamene akugwira ntchito ndi zakumwa zowoneka bwino kwambiri kapena zowonongeka. mtundu - kuti mupeze zotsatira zabwino kwambiri.
Magawo awiri ovuta kwambiri omwe amakhudza zotsatira za pipetting ndi zolondola komanso zolondola (Chithunzi 2) .Kuti mukwaniritse kulondola kwakukulu kwa pipetting, kulondola, ndi kudalirika, njira zingapo ziyenera kukumbukiridwa. Monga lamulo la thupi, wogwiritsa ntchito nthawi zonse ayenera kusankha pipette yaing'ono kwambiri yomwe ingathe kugwiritsira ntchito voliyumu yomwe ikufunidwa. µl yokhala ndi 5,000 µl pipette, zotsatira zake zingakhale zoipa. Zotsatira zabwino zingapezeke ndi 300 µl pipette, pamene 50 µl pipettes zimapereka zotsatira zabwino kwambiri. Kuwonjeza, voliyumu ya ma pipette achikhalidwe akhoza kusintha panthawi ya pitting chifukwa cha kuzungulira mwangozi kwa plunger. Ichi ndi chifukwa chake kusintha kwa ma pipette kumapangitsa kuti voliyumu isinthe. kuonetsetsa kulondola.Kulinganiza ndi mbali ina yofunika yomwe imathandiza kutsimikizira zotsatira zodalirika mwa kusonyeza kulondola ndi kulondola kwa pipette.Mchitidwewu ukhale wosavuta kwa wosuta; mwachitsanzo, ma pipette ena apakompyuta amatha kukhazikitsa zikumbutso za calibration, kapena kusunga mbiri ya calibration.Sizingongoganizira chabe.Ngati nsonga ya pipette imakhala yotayirira, ikutuluka, kapena ikugwa, ikhoza kuyambitsa mavuto osiyanasiyana. malo olakwika, kapenanso kuchititsa kuti nsonga igwe pansi pa pipette kwathunthu.Kusankha micropipette yapamwamba yopangidwa ndi malangizo enieni kumatsimikizira kugwirizana kotetezeka, kupereka mlingo wapamwamba wa kudalirika ndi zotsatira zabwino.
Pamalo ogwiritsira ntchito kwambiri, ndizofunikira kuti zikhale zogwira mtima momwe zingathere pamene mukusunga kudalirika ndi kusasinthasintha kwa ndondomeko ya pipetting.Pali njira zambiri zopititsira patsogolo mphamvu za pipetting, kuphatikizapo kugwiritsa ntchito ma pipettes a multichannel ndi / kapena electronic. monga kugawira mobwerezabwereza ndi bwino kugawa ma aliquots angapo a voliyumu yomweyo popanda kudzazanso nsonga.Kugwiritsa ntchito ma pipette a njira imodzi kusamutsa zitsanzo pakati pa mitundu yosiyanasiyana ya labware kumatha kukhala otopetsa kwambiri komanso olakwika.Mapaipi amtundu wa Multichannel amalola kusamutsa zitsanzo zambiri nthawi imodzi mu kuphethira kwa diso.Sikuti izi zimangowonjezera kuvulala kwa pipeti (ndikuthandizira kubwerezabwereza) pipettes ngakhale amatha kusiyanasiyana nsonga katayanitsidwe pa pipetting, kulola anasamutsidwa kufanana angapo zitsanzo pakati pa kukula labware osiyana ndi akamagwiritsa, kupulumutsa maola nthawi (Chithunzi 3).
Akatswiri a zasayansi nthawi zambiri amathera maola ambiri tsiku ndi tsiku. Izi zingayambitse kusapeza bwino ndipo, zikavuta kwambiri, ngakhale kuvulala kwa dzanja kapena mkono. Malangizo abwino kwambiri opewera ngozi zomwe zingatheke ndi kuchepetsa nthawi yomwe mumagwiritsa ntchito pipette mpaka nthawi yaifupi kwambiri. gwira mapangidwe, ndikusintha voliyumu momasuka komanso mwachangu momwe mungathere kuti mupewe kusuntha kosafunikira.Komanso, malangizowo ndi ofunikira, monga kukweza nsonga ndi ejection nthawi zambiri zimafuna mphamvu zambiri kuposa pipetting ndipo pali chiopsezo chovulaza, makamaka pazikhazikiko zapamwamba.Nsonga za Pipette ziyenera kugwedezeka ndi mphamvu zochepa, kupereka kugwirizanitsa kotetezeka, ndi kukhala kosavuta kutulutsa mofanana.
Posankha micropipette yoyenera pa ntchito yanu, ndikofunika kuyang'ana mbali iliyonse ya kayendetsedwe ka ntchito yanu.Poganizira za pipette, makhalidwe ake, mtundu ndi kuchuluka kwa madzi omwe akuponyedwa paipi, ndi malangizo omwe amagwiritsidwa ntchito, asayansi akhoza kutsimikizira zotsatira zolondola, zolondola komanso zodalirika pamene akukhalabe ndi zokolola komanso kuchepetsa chiopsezo cha kuvulala.
M'kopeli, kubwezeretsedwa kwa ma analytes oyambira kumawunikidwa ndi HPLC-MS pogwiritsa ntchito makina osakanikirana amphamvu a SPE microplates. Ubwino wa SEC-MALLS pakugwiritsa ntchito biopharmaceutical…
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Nthawi yotumiza: Jun-10-2022