Cov kws tshaj lij hauv chav kuaj tuaj yeem siv sij hawm txhua hnub los tuav lub micropipette, thiab txhim kho pipetting efficiency thiab ua kom ntseeg tau tias cov txiaj ntsig zoo feem ntau yog ib qho nyuaj.Kev xaiv lub micropipette txoj cai rau txhua daim ntawv thov yog qhov tseem ceeb rau kev ua tiav ntawm kev ua haujlwm hauv chav kuaj; nws tsis tsuas yog ua kom cov kev ua tau zoo ntawm ib qho kev sim, tab sis kuj nce efficiency.Kev nkag siab txog cov kev xav tau ntawm pipetting workflow enables cov neeg siv xaiv qhov tseeb thiab rov ua dua pipettes, tab sis muaj ntau lwm yam uas yuav tsum tau txiav txim siab los txhim kho cov txiaj ntsig pipetting thiab lav qhov kev ua tiav ntawm kev sim.
Kev hais lus dav dav, cov kua dej poob rau hauv peb pawg tseem ceeb: aqueous, viscous, thiab volatile.Feem ntau cov kua yog dej-raws li, ua rau huab cua hloov pipettes thawj xaiv rau ntau.Thaum cov kua feem ntau ua haujlwm zoo nrog hom pipette, volumetric pipettes yuav tsum tau xaiv thaum ua hauj lwm nrog cov kua nplaum heev los yog volatile kua. pipetting txheej txheem - tsis hais hom kua - kom tau zoo heev.
Qhov ob qhov tseem ceeb tshaj plaws uas cuam tshuam rau cov txiaj ntsig pipetting yog qhov tseeb thiab qhov tseeb (Daim duab 2).Yuav kom ua tiav qhov siab tshaj plaws pipetting raug, precision, thiab kev ntseeg tau, ntau cov txheej txheem yuav tsum tau khaws cia rau hauv siab. Raws li txoj cai ntawm tus ntiv tes xoo, tus neeg siv yuav tsum xaiv qhov tsawg tshaj plaws pipette uas tuaj yeem ua raws li qhov xav tau hloov ntim.Qhov no yog qhov tseem ceeb raws li qhov raug txo qis raws li qhov ntim ntawm cov kav dej tsawg kawg yog 0. µl nrog 5,000 µl pipette, cov txiaj ntsig yuav tsis zoo. Cov txiaj ntsig zoo tuaj yeem tau txais nrog 300 µl pipettes, thaum 50 µl pipettes muab cov txiaj ntsig zoo tshaj plaws. Tsis tas li ntawd, cov ntim ntim ntawm cov khoom siv phau ntawv pipettes tuaj yeem hloov pauv thaum lub raj xa dej vim yog kev sib hloov ntawm lub plunger. kom paub tseeb tias qhov tseeb.Calibration yog lwm qhov tseem ceeb uas yuav pab tau kom ntseeg tau cov txiaj ntsig zoo los ntawm kev ua kom pom qhov tseeb thiab qhov tseeb ntawm cov pipette.Qhov txheej txheem no yuav tsum yooj yim rau cov neeg siv; Piv txwv li, qee cov khoom siv hluav taws xob tuaj yeem tsim cov lus ceeb toom calibration, lossis txuag cov keeb kwm calibration.Nws tsis yog pipettes los txiav txim siab.Yog hais tias lub pipette taub hau yuav xoob, xau, los yog ntog, nws tuaj yeem ua rau muaj ntau yam teeb meem.Qhov teeb meem tshwm sim hauv chav kuaj no feem ntau tshwm sim los ntawm kev siv cov lus qhia dav dav pipette, uas feem ntau xav tau "tapping." Cov txheej txheem no yuav ua rau lub qhov dej ntws tawm los yog ua rau lub qhov muag tsis pom. lub taub hau kom poob tawm ntawm lub pipette nkaus xwb.Xaiv lub siab zoo micropipette tsim nrog cov lus qhia tshwj xeeb kom muaj kev ruaj ntseg zoo dua, muab qib siab ntawm kev ntseeg siab thiab cov txiaj ntsig zoo dua. Tsis tas li ntawd, ib yam dab tsi yooj yim li xim-coding pipettes thiab cov lus qhia kuj tuaj yeem pab cov neeg siv kom paub meej cov lus qhia raug xaiv rau lawv cov pipettes.
Nyob rau hauv ib puag ncig high-throughput, nws yog ib qho tseem ceeb kom ua tau raws li qhov ua tau zoo thaum tswj kev ntseeg tau thiab sib xws ntawm cov txheej txheem pipetting.Muaj ntau txoj hauv kev los txhim kho pipetting efficiency, nrog rau kev siv ntau channel thiab/los yog hluav taws xob pipettes.Cov cuab yeej siv tau ntau yam no feem ntau muaj ntau yam kev sib txawv pipetting-xws li thim rov qab pipetting, kev hloov pauv mus rau qhov kev pab cuam ntau dua, thiab ntau yam kev pab cuam. process.Piv txwv li, cov txheej txheem xws li rov xa rov qab yog qhov zoo tshaj plaws rau kev xa tawm ntau lub aliquots ntawm tib lub ntim yam tsis tau rov ua dua cov lus qhia.Siv ib leeg-channel pipettes hloov cov qauv ntawm cov qauv sib txawv ntawm labware tuaj yeem ua sai heev thiab yuam kev.Multichannel pipettes tso cai hloov ntau cov qauv ib zaug hauv lub qhov muag. Tsis tsuas yog blinker qhov kev ua tau zoo. thiab kev raug mob rov ua dua (RSI).Qee pipettes txawm muaj peev xwm sib txawv qhov sib txawv ntawm qhov sib txawv thaum lub sij hawm pipetting, tso cai rau kev hloov mus rau tib lub sij hawm ntawm ntau qhov sib txawv ntawm qhov sib txawv labware qhov ntau thiab tsawg, txuag sij hawm (Daim duab 3).
Cov kws tshaj lij hauv chav kuaj feem ntau siv sijhawm ntau teev hauv ib hnub pipetting.Qhov no tuaj yeem ua rau tsis xis nyob thiab, qhov mob hnyav dua, txawm tias txhais tes lossis caj npab raug mob.Cov lus qhia zoo tshaj plaws kom tsis txhob muaj qhov txaus ntshai no yog txo lub sijhawm koj tuav lub pipette rau lub sijhawm luv tshaj plaws.Ntxiv rau qhov no, cov neeg siv yuav tsum xaiv lub teeb yuag thiab zoo sib npaug micropipette nrog huab hwm coj nyob rau hauv qhov chaw kom haum rau cov neeg siv khoom zoo dua, lub pipette zoo yuav tsum muaj kev ruaj ntseg zoo. tsim, thiab kho lub ntim kom yooj yim thiab sai li sai tau kom tsis txhob muaj kev txav txav tsis tsim nyog.Tsis tas li ntawd, cov lus qhia tseem ceeb, vim tias qhov chaw thau khoom thiab ejection feem ntau yuav tsum tau muaj zog ntau dua li pipetting thiab muaj feem yuav raug mob, tshwj xeeb tshaj yog nyob rau hauv high-throughput settings.Pipette cov lus qhia yuav tsum snap rau hauv qhov chaw nrog tsawg kawg nkaus quab yuam, muab kev ruaj ntseg kev twb kev txuas, thiab sib npaug yooj yim rau eject.
Thaum xaiv qhov zoo micropipette rau koj daim ntawv thov, nws yog ib qho tseem ceeb uas yuav tsum tau saib xyuas txhua yam ntawm koj qhov kev ua haujlwm.Los ntawm kev xav txog cov pipette, nws cov yam ntxwv, hom thiab ntim ntawm cov kua yog pipetted, thiab cov lus qhia siv, cov kws tshawb fawb tuaj yeem lav qhov tseeb, meej thiab txhim khu kev qha thaum tswj kev tsim khoom thiab txo qhov kev pheej hmoo ntawm kev raug mob.
Hauv tsab ntawv no, kev rov qab los ntawm cov tshuaj ntsuam xyuas yooj yim yog ntsuas los ntawm HPLC-MS siv cov sib xyaw-hom muaj zog cation txauv SPE microplates. Cov txiaj ntsig ntawm SEC-MALLS hauv kev siv biopharmaceutical…
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Lub sij hawm xa tuaj: Jun-10-2022