Ndiani asingazive acetone, ethanol & co.kutanga kubuda kunzepipette tipzvakananga mushure mekuda?Pamwe, mumwe nemumwe wedu akambosangana nazvo.Zvinofungidzirwa mabikirwo epachivande senge "kushanda nekukurumidza sezvinobvira" uku "kuisa machubhu padhuze nepadhuze kudzivirira kurasikirwa kwekemikari uye kurasika" ndezvemaitiro ako ezuva nezuva?Kunyangwe kana madonhwe emakemikari akamhanya nekukurumidza, zvinowanzo bvumidzwa kuti pipetting haisiriyo chaiyo.Kungochinja kudiki kwemaitiro epipetting, uye sarudzo yakanaka yepipette mhando inogona kubatsira kukunda matambudziko aya ezuva nezuva!
Sei pipettes ichidonha?
Mapipeti echinyakare anotanga kudonha kana poipi inoputika zvinwiwa nekuda kwemhepo mukati mepipette.Iyi inonzi air cushion iripo pakati pemvura yemuenzaniso uye piston mukati mepipette.Sezvinowanzozivikanwa, mweya unochinjika uye unochinjika kune zvekunze zvinofurira senge tembiricha uye mhepo yekumanikidza nekuwedzera kana kumanikidza.Zvinwiwa zvinoiswawo pasi pemaitiro ekunze uye zvinongobuda mumhepo sezvo hunyoro hwemhepo hwadzikira.Mvura inoputika inoputika nekukurumidza kupfuura mvura.Panguva yepipetting, inobuda mucushion yemhepo ichimanikidza iyo yekupedzisira kuti iwedzere uye mvura inotsikirirwa kunze kwepipette tip ... iyo pipette inodonha.
Nzira yekudzivirira sei mvura kuti isadonha
Imwe nzira yekudzikisa kana kumisa kudonha ndeyekuwana huwandu hwepamusoro hwehunyoro mumutsara wemhepo.Izvi zvinoitwa ne pre-wetting thepipette tipuye nokudaro achizadza cushion yemhepo.Paunenge uchishandisa zvinwiwa zvakaderera zvakaita se70 % Ethanol kana 1 % acetone, shuvira uye ubvise mvura yemuenzaniso kanenge ka3, usati washuvira vhoriyamu yekuenzanisa yaunoda kuendesa.Kana huwandu hwemvura inovhuvhuta yakakwira, dzokorora izvi pre-wetting cycles 5-8 times.Nekudaro, nekuwanda kwakanyanya senge 100 % ethanol kana chloroform, izvi hazvizove zvakakwana.Zviri nani kushandisa imwe mhando yepipette: yakanaka displacement pipette.Aya mapipette anoshandisa matipi ane yakasanganiswa piston isina mhepo cushion.Sample yakanangana nepiston uye hapana njodzi yekudonha.
Iva nyanzvi yepipetting
Iwe unogona nyore kuvandudza iko kurongeka kwako paunenge uchiputira zvinwiwa zvine simba nekusarudza nzira chaiyo kana kushandura chishandiso chauri kushandisa.Pamusoro pezvo, iwe unozowedzera kuchengetedzeka nekudzivirira kudurura uye kurerutsa mafambiro ako.
Nguva yekutumira: Jan-17-2023