Inzobere muri laboratoire zirashobora kumara amasaha buri munsi zifata micropipette, no kunoza imikorere ya pipine no kwemeza ibisubizo byizewe akenshi ni ikibazo. Guhitamo micropipette ibereye kubisabwa byose ni urufunguzo rwo gutsinda imirimo ya laboratoire; ntabwo yemeza gusa imikorere yubushakashatsi ubwo aribwo bwose, ariko kandi byongera imikorere.Kutumva ibikenewe byogukora imiyoboro ituma abayikoresha bahitamo imiyoboro nyayo kandi isubirwamo, ariko hariho nibindi bintu byinshi bigomba kwitabwaho kugirango tunonosore ibisubizo kandi byemeze ko ubushakashatsi bwatsinze.
Muri rusange, ibibyimba biri mubyiciro bitatu byingenzi: amazi, ibishishwa, kandi bihindagurika. Amazi menshi ashingiye kumazi, bigatuma imiyoboro yo kwimura ikirere ihitamo bwa mbere kuri benshi.Mu gihe amazi menshi akora neza hamwe nubwoko bwa pipete, imiyoboro ya volumetric igomba guhitamo mugihe ikorana nubwoko bwamazi cyane kandi ikanatandukana. - kubisubizo byiza.
Ibipimo bibiri byingenzi bigira ingaruka kubisubizo byogusohora ni ukuri kandi neza (Igicapo 2) .Kugera kumurongo ntarengwa wogusohora neza, neza, no kwizerwa, hagomba kuzirikanwa ibintu byinshi. Nkuko amategeko abigenga, uyikoresha agomba guhora ahitamo umuyoboro muto muto ushobora gutwara ingano yifuzwa. Ibi nibyingenzi kuko kugabanuka kugabanuka nkuko urugero rwashizweho ruri hafi ya pisine. bikennye.Ibisubizo byiza birashobora kuboneka hamwe na 300 µl pipettes, mugihe 50 µl imiyoboro itanga ibisubizo byiza.Ikindi kandi, ingano yashyizwe kumiyoboro gakondo yintoki irashobora guhinduka mugihe cyo gutembera bitewe nimpanuka zimpanuka za plunger.Niyo mpamvu rero bamwe mubakora imiyoboro ya pipine bashizeho uburyo bwo gufunga ibipimo bifatika kugirango babone impinduka zukuri kandi zifatika kugirango berekane neza ko ari ngombwa. pipette. Iyi nzira igomba kuba yoroshye kubakoresha; kurugero, imiyoboro imwe ya elegitoronike irashobora gushiraho kwibutsa kalibrasi, cyangwa kubika amateka ya kalibrasi.Ntabwo ari imiyoboro gusa yo gutekerezaho.Niba umuyoboro wa pipette uhindutse urekuye, ugatemba, cyangwa ukagwa, birashobora gutera ibibazo bitandukanye. Iki kibazo gikunze kugaragara muri laboratoire gikunze guterwa no gukoresha inama rusange ya pipeti, akenshi ikaba isaba “gukubita no gukanda. umuyoboro wuzuye. Guhitamo micropipette yo mu rwego rwohejuru yateguwe hamwe ninama zihariye zituma habaho umutekano wizewe, utanga urwego rwo hejuru rwo kwizerwa hamwe nibisubizo byiza. Byongeye kandi, ikintu cyoroshye nkibikoresho byerekana amabara hamwe ninama bishobora gufasha abakoresha kwemeza ko inama zukuri zatoranijwe kuri pipeti zabo.
Mubidukikije byinjira cyane, ni ngombwa gukora neza bishoboka mugihe ukomeje kwizerwa no guhuzagurika mubikorwa byogutwara imiyoboro.Hariho inzira nyinshi zo kunoza imikorere yimiyoboro, harimo no gukoresha imiyoboro myinshi hamwe na / cyangwa imiyoboro ya elegitoronike. Ibi bikoresho bitandukanye bikunze gutanga uburyo butandukanye bwo gutumatumanaho-nko guhinduranya ibintu, uburyo bwo gusubiramo ibintu, nkuburyo bukoreshwa muburyo butandukanye. gutanga alikoti nyinshi zingana zingana utarinze kuzuza inama. Gukoresha imiyoboro imwe yumuyoboro umwe kugirango wohereze ingero hagati yuburyo butandukanye bwa laboratoire irashobora guhita irambirana cyane kandi ikunda kwibeshya. ihererekanyabubasha ryinshi hagati yubunini bwa labware nuburyo butandukanye, bizigama amasaha yigihe (Ishusho 3).
Inzobere muri laboratoire zisanzwe zimara amasaha kumunsi zipompa.Ibi birashobora gutera ikibazo kandi, mugihe gikomeye cyane, ndetse no gukomeretsa amaboko cyangwa ukuboko. Inama nziza yo kwirinda izo ngaruka zishobora kugerwaho ni ukugabanya igihe ufata pipeti mugihe gito gishoboka. Usibye ibi, abakoresha bagomba guhitamo micropipette yoroheje kandi iringaniye hamwe na misa mukigo cyiza kandi gikwiye neza neza. hindura amajwi neza kandi byihuse bishoboka kugirango wirinde kugenda bitari ngombwa.Ikindi kandi, inama ni ngombwa, kuko inama yo gupakira no gusohora akenshi bisaba imbaraga zirenze imiyoboro kandi hari impanuka zishobora gukomeretsa, cyane cyane mumashanyarazi menshi.
Mugihe uhisemo micropipette ibereye kubyo usaba, ni ngombwa kureba kuri buri kintu cyose cyakazi cyawe. Urebye pipette, ibiyiranga, ubwoko nubunini bwamazi arimo gutwarwa, hamwe ninama zikoreshwa, abahanga barashobora kwemeza ibisubizo nyabyo, byuzuye kandi byizewe mugukomeza umusaruro no kugabanya ingaruka zo gukomeretsa.
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Igihe cyo kohereza: Jun-10-2022