Kodi Cryovials ndi chiyani?
Cryogenic yosungirako Mbalendi zotengera zing'onozing'ono, zokhala ndi cylindrical zomwe zimapangidwira kusunga ndi kusunga zitsanzo pa kutentha kwambiri.Ngakhale mwamwambo mbalezi zidapangidwa kuchokera kugalasi, tsopano zimapangidwa kwambiri kuchokera ku polypropylene kuti zitheke komanso chifukwa chamtengo.Ma Cryovials adapangidwa mosamala kuti azitha kupirira kutentha mpaka -196 ℃, komanso kuti azitha kutengera mitundu yosiyanasiyana ya ma cell.Izi zimasiyana kuchokera ku ma cell stem cell, ma microorganisms, ma cell oyambira mpaka ma cell okhazikika.Kupitilira apo, pangakhalenso zamoyo zing'onozing'ono zomwe zimasungidwa mkaticryogenic yosungirako mbale, komanso nucleic acid ndi mapuloteni omwe amafunika kusungidwa pa kutentha kwa cryogenic yosungirako.
Mbale zosungirako za cryogenic zimabwera m'njira zosiyanasiyana, ndipo kupeza mtundu wolondola womwe umakwaniritsa zosowa zanu zonse kuonetsetsa kuti mukusunga kukhulupirika kwachitsanzo popanda kubweza.Werengani m'nkhani yathu kuti mudziwe zambiri za zofunikira zogulira posankha cryovial yoyenera kuti mugwiritse ntchito labotale.
Katundu wa Cryogenic Vial Kuti Muganizire
Zakunja vs Ulusi Wamkati
Anthu nthawi zambiri amasankha izi potengera zomwe amakonda, koma pali kusiyana kwakukulu koyenera kuganizira pakati pa mitundu iwiri ya ulusi.
Ma laboratories ambiri nthawi zambiri amasankha mabotolo olowa mkati kuti achepetse malo osungiramo machubu kuti azitha kulowa bwino m'mabokosi afiriji.Ngakhale zili choncho, mutha kuganiza kuti njira yopangira ulusi wakunja ndiyo njira yabwino kwa inu.Amaonedwa kuti ali ndi chiopsezo chochepa cha kuipitsidwa, chifukwa cha mapangidwe omwe amachititsa kuti zikhale zovuta kwambiri kuti china chilichonse kupatula chitsanzo chilowe mu vial.
Mbale zokhala ndi ulusi wakunja nthawi zambiri zimakonda kugwiritsidwa ntchito ndi ma genomic, koma njira iliyonse imatengedwa kuti ndiyoyenera kugwiritsa ntchito biobanking ndi mapulogalamu ena apamwamba.
Chinthu chomaliza choyenera kuganizira pa ulusi - ngati labotale yanu imagwiritsa ntchito makina, mungafunike kuganizira ulusi womwe ungagwiritsidwe ntchito ndi zida zogwiritsira ntchito.
Voliyumu Yosungira
Mbale za cryogenic zimapezeka mosiyanasiyana kuti zikwaniritse zosowa zambiri, koma nthawi zambiri zimakhala pakati pa 1 mL ndi 5 mL.
Chofunika ndikuwonetsetsa kuti cryovial yanu siidzaza komanso kuti pali malo owonjezera, ngati chitsanzocho chifufuma pamene mukuzizira.M'machitidwe, izi zikutanthauza kuti ma laboratories amasankha 1 mL Mbale posunga zitsanzo za 0.5 mL ya maselo oimitsidwa mu cryoprotectant, ndi 2.0 mL Mbale kwa 1.0 mL ya zitsanzo.nsonga ina yoti musadzaze mochulukira m'mbale zanu ndikupangitsani kuti mugwiritse ntchito ma cryovials okhala ndi zilembo zomaliza, zomwe zimatsimikizira kuti mupewe kutupa komwe kungayambitse kusweka kapena kutayikira.
Screw Cap vs Flip Top
Mtundu wa pamwamba womwe mumasankha umadalira makamaka ngati mukugwiritsa ntchito madzi a nayitrogeni kapena ayi.Ngati ndi choncho, ndiye kuti mudzafunika ma cryovials okhala ndi phula.Izi zimatsimikizira kuti sizingatseguke mwangozi chifukwa cha kusagwira bwino kapena kusintha kwa kutentha.Kuphatikiza apo, ma screw caps amalola kubweza mosavuta kumabokosi a cryogenic ndikusungirako bwino.
Komabe, ngati simukugwiritsa ntchito nayitrogeni wamadzimadzi ndipo mukusowa pamwamba pomwe ndi yosavuta kutsegula, ndiye kuti flip top ndiyo njira yabwinoko.Izi zidzakupulumutsirani nthawi yochuluka chifukwa ndizosavuta kutsegula, zomwe zimapangitsa kuti zikhale zothandiza kwambiri pamachitidwe apamwamba komanso omwe amagwiritsa ntchito njira za batch.
Seal Security
Njira yabwino yotsimikizira chisindikizo chotetezedwa ndikuwonetsetsa kuti chipewa chanu cha cryovial ndi botolo zonse zidapangidwa kuchokera kuzinthu zomwezo.Izi zidzaonetsetsa kuti akuchepa ndikukula pamodzi.Ngati apangidwa pogwiritsa ntchito zipangizo zosiyanasiyana, ndiye kuti adzachepa ndikukula mosiyanasiyana pamene kutentha kumasintha, mipata yotsogolera ndi kutayikira komwe kungathe kuchitika ndi kuipitsidwa kotsatira.
Makampani ena amapereka makina ochapira awiri ndi flange kuti akhale ndi chitetezo chapamwamba kwambiri pama cryovial opangidwa ndi kunja.O-Ring cryovials amaonedwa kuti ndi odalirika kwambiri kwa ma cryovial okhala ndi ulusi wamkati.
Galasi vs Pulasitiki
Kuti atetezeke komanso kuti asavutike, ma laboratories ambiri tsopano amagwiritsa ntchito pulasitiki, nthawi zambiri polypropylene, m'malo mwa ma ampule agalasi otsekedwa ndi kutentha.Ma ampule agalasi tsopano amaonedwa kuti ndi chisankho chachikale chifukwa panthawi yosindikiza kutulutsa kosawoneka kwa pinhole kumatha kuchitika, komwe kumasungunuka pambuyo posungidwa mu nayitrogeni wamadzimadzi kungayambitse kuphulika.Komanso sizoyenera kutengera njira zamakono zolembera, zomwe ndizofunikira pakuwonetsetsa kuti zitsanzo zikuyenda bwino.
Self Standing vs Zozungulira Zozungulira
Mbale za cryogenic zimapezeka zonse ngati zodziyimira zokha ndi zapansi zooneka ngati nyenyezi, kapena ngati zozungulira.Ngati muyenera kuyika mbale zanu pamtunda ndiye onetsetsani kuti mwasankha kudziyimira pawokha
Tsatanetsatane ndi Kutsata Zitsanzo
Malo osungiramo cryogenic nthawi zambiri amanyalanyazidwa koma kutsatiridwa ndi kutsata zitsanzo ndizofunikira kwambiri kuziganizira.Zitsanzo za cryogenic zikhoza kusungidwa kwa zaka zambiri, panthawi yomwe ogwira ntchito amatha kusintha ndipo popanda zolemba zosungidwa bwino akhoza kukhala osadziwika.
Onetsetsani kuti mwasankha Mbale zomwe zimapanga chizindikiritso chachitsanzo chosavuta momwe mungathere.Zinthu zomwe muyenera kuziganizira ndi izi:
Malo akuluakulu olemberamo kuti alembe zambiri zokwanira kuti zolemba zitha kupezeka ngati vial ili pamalo olakwika - nthawi zambiri chizindikiritso cha selo, tsiku loyimitsidwa, ndi zilembo zoyambira za munthu yemwe ali ndi udindo ndizokwanira.
Ma barcode kuti athandizire kasamalidwe ka zitsanzo ndi njira zotsatirira
Zovala zamitundu
Chidziwitso chamtsogolo - tchipisi tosamva kuzizira kwambiri zikupangidwa zomwe, zikayikidwa mkati mwa ma cryovial, zitha kusunga mbiri yatsatanetsatane yamafuta komanso zambiri za batch, zotsatira zoyesa ndi zolemba zina zoyenera.
Kuphatikiza pa kulingalira za mitundu yosiyanasiyana ya mbale zomwe zilipo, lingaliro lina liyeneranso kuperekedwa ku njira yaukadaulo yosungira ma cryovial mu nayitrogeni wamadzimadzi.
Kutentha Kosungirako
Pali njira zingapo zosungirako zosungirako za cryogenic za zitsanzo, iliyonse imagwira ntchito pa kutentha kwapadera.Zosankha komanso kutentha komwe amagwira ntchito ndi izi:
Gawo lamadzi LN2: sungani kutentha kwa -196 ℃
Nthunzi gawo LN2: amatha kugwira ntchito pa kutentha kwapadera pakati pa -135 ° C ndi -190 ° C kutengera chitsanzo.
Mufiriji wa nayitrojeni wa nthunzi: -20°C mpaka -150°C
Mtundu wa ma cell omwe akusungidwa komanso njira yosungira yomwe wofufuza amasankha ndizomwe angasankhe mwa njira zitatu zomwe labotale yanu imagwiritsa ntchito.
Komabe, chifukwa cha kutentha kochepa kwambiri komwe kumagwiritsidwa ntchito si machubu onse kapena mapangidwe omwe angakhale abwino kapena otetezeka.Zida zimatha kukhala zolimba kwambiri pakutentha kwambiri, kugwiritsa ntchito vial yosayenerera kutentha komwe mwasankha kungapangitse chombocho kusweka kapena kusweka posungira kapena kusungunuka.
Yang'anani mosamalitsa malingaliro a opanga kuti agwiritse ntchito moyenera chifukwa mbale zina za cryogenic ndizoyenera kutentha kwa -175 ° C, zina -150 ° C zina 80 ° C.
Ndizofunikanso kudziwa kuti opanga ambiri amanena kuti mbale zawo za cryogenic siziyenera kumizidwa mu gawo lamadzimadzi.Ngati Mbale izi zasungidwa mu madzi gawo pamene kubwerera ku firiji Mbale izi kapena kapu zosindikizira akhoza kusweka chifukwa mofulumira kumanga-mmwamba kuthamanga chifukwa cha kutayikira pang'ono.
Ngati maselo kusungidwa mu madzi gawo la madzi asafe, ganizirani kusunga maselo abwino cryogenic Mbale kutentha losindikizidwa mu cryoflex tubing kapena kusunga maselo mu galasi ampules kuti hermetically chatsekedwa.
Nthawi yotumiza: Nov-25-2022