Hoʻololi o nā ʻōnaehana paipu a me nā ʻenehana

Hoʻohana ʻia i ka wai waipili i ka hoʻohana ʻana i nā ʻōnaehana automated ma kahi o ka hana lima e hoʻoili i nā wai ma waena o nā wahi. I loko o nā keʻena noiʻi biological, ʻokoʻa ka nui o ka hoʻoili wai maʻamau mai0.5 μL i ka 1 mL, ʻoiai ua koi ʻia nā hoʻoili pae nanoliter i kekahi mau noi. ʻOkoʻa nā ʻōnaehana lawelawe wai ʻokoʻa i ka nui, paʻakikī, hana, a me ke kumukūʻai.

Mai ka lima a hiki i ka lawelawe ʻana i ka wai

ʻO ka mea hana maʻamau kapipette manual—he mea paʻa lima e koi ana i ka hana hou ʻana o ka mea hoʻohana no kēlā me kēia hana (aspiration and dispensing). Hiki i ka hoʻohana lōʻihi ke alakaʻi i nā ʻeha ʻeha e like mecarpal tunnel syndrome.

ʻO nā paipu uilahōʻike i ke kaʻina evolutionary e hiki mai ana. Hiki i nā pipette manual a me ka uila ke loaʻa nā leo hoʻololi/paʻa a me nā kaha 1-16. ʻOiai e hoʻonui ka nui o nā pipette uila uila i ka hoʻohālikelike ʻia me nā pipettes single-channel manual, ua kaupalena ʻia lākou e ka hoʻokomo kanaka.ʻO nā mea hāʻawi ʻakomie lanakila i kēia ma ka puʻunaue like ʻana i ka wai i loko o nā pūnāwai a pau o kahi microplate (e laʻa, 96- a i ʻole 384-well papa).

Pono pinepine nā hoʻokolohua lab hou i nā "kaʻina hana."ʻO nā keʻena hana hoʻokele wai ʻakomihoʻohui i nā modula (e laʻa, nā haʻalulu, nā mea hoʻomehana) a me nā polokalamu e hoʻokō i nā kuʻina paʻakikī.

• Pūnaehana komopaʻa me ka polokalamu hoʻohana-aloha akā hiki ke maʻalahi.
• ʻōnaehana holomuakākoʻo i nā hoʻomaikaʻi modular, hoʻonui i nā kahe hana, a me ka hoʻohui ʻana me nā mea hana lab ʻē aʻe.

ʻO nā kumu nui no ke koho ʻana i ka ʻenehana mālama wai:
(i) Throughput, (ii) Workflow complex, (iii) Budget, (iv) Lab space, (v) Sterility/cross-contamination control, (vi) Traceability, (vii) Precision.

ʻO ka pololei i ka lawelawe ʻana i ka wai wai

Pili ka pololei i nā waiwai wai, ka ʻenehana pipetting, a (no nā ʻōnaehana manual) ka mākaukau hoʻohana. ʻO nā waiwai wai—i hoʻopili ʻia e ka mahana, ke kaomi, a me ka haʻahaʻa—e like me:

• ʻO ka viscosity(hana kahe)
• ʻO ka mānoanoa(ka nui / ʻāpana leo)
• Hoʻopili/koheʻe(pilikia)
• ʻO ka ʻili o ka ʻili
• Pumi mahu

Hoʻoponopono nā ʻōnaehana kiʻekiʻe i nā ʻāpana e helu i kēia mau waiwai:
(i) ʻO ka wikiwiki o ka manaʻo / hāʻawi ʻana,
(ii) ʻAha ea
(iii) Ka manawa noho ma mua o ka manaʻo,
(iv) ʻO ka wikiwiki hoʻihoʻi ʻana.

Nā ʻenehana paipu nui

Hoʻokaʻawale ʻia e nā mīkini hoʻoulu wai:

1. Hoʻoneʻe Lewa
2. Ka hoʻoneʻe wai
3. Hoʻoneʻe maikaʻi
4. ʻenehana Acoustic

Evolution Timeline

Pipette Manuahi (Kānana Hoʻokahi) → Pipette Manual (Multi-Channel) → Pipette Electronic → Dispenser Automated → Ke kahua hana komo-level