Automated Liquid Handlingzvinoreva kushandiswa kwemaotomatiki masisitimu pachinzvimbo chebasa remaoko kuendesa zvinwiwa pakati penzvimbo. Mubiological research labs, akajairwa ekufambisa mvura mavhoriyamu anotangira kubva0.5 μL kusvika 1 mL, kunyangwe nanoliter-level kutamiswa kunodiwa mune mamwe maapplication. Automated liquid inobata masisitimu anosiyana muhukuru, kuoma, kuita, uye mutengo.
Kubva kuBhuku kusvika kune Automated Liquid Handling
Chishandiso chinonyanya kukosha ndeyemanual pipette-chishandiso chinobata ruoko chinoda kudzokorora mushandisi kupindira kune yega yega nhanho (kushuvira uye kugovera). Kushandiswa kwenguva refu kunogona kutungamirira kune kudzokorora kukuvara kwakafananacarpal tunnel syndrome.
Electronic pipettesmiririra danho rinotevera rekushanduka-shanduka. Ose ari maviri manyorero uye emagetsi pipette anogona kuve anogadziriswa / akagadziriswa mavhoriyamu uye 1-16 chiteshi. Nepo ma-multi-channel electronic pipettes anowedzera kubuda kana achienzaniswa nemanual single-channel pipettes, anoramba akaganhurirwa nekuisa kwevanhu.Automated dispenserskukunda izvi nekugovera mvura panguva imwe chete mumatsime ese emicroplate (semuenzaniso, 96- kana 384-tsime mahwendefa).
Yemazuva ano lab assays kazhinji inoda akawanda-nhanho "workflows."Automated liquid inobata nzvimbo dzekushandirabatanidza mamodule (semuenzaniso, shakers, heaters) uye software yekuita maprotocol akaoma.
- Entry-level systemsari compact ne-user-friendly software asi zvishoma kushanduka.
- Advanced systemstsigira modular kukwidziridzwa, yakawedzera workflows, uye kubatanidzwa nemimwe michina yeabhu.
Zvinhu zvakakosha pakusarudza tekinoroji yekubata mvura zvinosanganisira:
(i) Kuburikidza, (ii) Kuomarara kwebasa, (iii) Budget, (iv) Nzvimbo yeLab, (v) Kusabereka/kukanganisa kusvibiswa, (vi) Traceability, (vii) Precision.
Precision in Automated Liquid Handling
Kurongeka kunoenderana nemidziyo yemvura, nzira yepipetting, uye (yemanyorero masisitimu) hunyanzvi hwemushandisi. Liquid zvivakwa-zvinokonzerwa nekushisa, kudzvanywa, uye humidity-zvinosanganisira:
- Viscosity(kuyerera maitiro)
- Density(huremu / chiyero cheyuniti)
- Kunamatira/kubatana(kunamira)
- Surface tension
- Mhepo pressure
Yepamberi masisitimu anogadzirisa ma parameter kuti azvidavirire kune izvi zvivakwa:
(i) Kushuvira / kugovera kumhanya,
(ii) Mhepo mapundu (kuvhuvhuta/kutamiswa kwemhepo),
(iii) Pre-aspiration kugara nguva,
(iv) Zano rekubvisa kumhanya.
Makuru Pipetting Technologies
Inokamurwa ne liquid propulsion mechanisms:
- Air Displacement
- Liquid Displacement
- Positive Displacement
- Acoustic Technology
Evolution Timeline
Manual Pipette (Iyo-Channel) → Manual Pipette (Multi-Channel) → Yemagetsi Pipette → Automated Dispenser → Yekupinda-Level Workstation → Modular Automated Workstation
| Pipetting Technology | Key Features | Primary Applications |
| Air Displacement | Mhepo cushion inoparadzanisa piston inofamba kubva kumuenzaniso | Yakagadzikana zvakanyanya pamavhoriyamu mukati me 0.5-1,000 μl |
| Liquid Displacement | Mhepo cushion inopatsanura system fluid kubva kumuenzaniso | Inowanzoshandiswa neyakagadziriswa simbi isina tsvina inogezeswa mazano; yakanakira matanho anoda akaboorwa machubhu |
| Positive Displacement | Kusangana kwakananga pakati pekufambisa piston uye sampuli | Inofarirwa ye-high-viscosity uye sampuli dzisina kusimba |
| Acoustic Technology | Kusabatika kwemvura kutamisa uchishandisa acoustic simba (masaisai anonzwika) | Ultra-yakaderera mavhoriyamu (kudzika kune nanoliter renji) |
Nguva yekutumira: May-12-2025