E ka tšeptjoangmalebela a pipetteetsa bonnete ba ho sebetsana le metsi ka nepo, ho sireletsa liteko tsa hau liphosong. Malebela a boleng bo tlase a ka baka ho lutla, litekanyo tse sa nepahalang, kapa tšilafalo. Mohlala, ho ikamahanya ho sa nepahalang ho ka lebisa tahlehelong ea sampole, ha malebela a senyehileng a sekisetsa botšepehi ba data. Kamehla khetha malebela a boleng bo phahameng ba pipette ho boloka botsitso. Bakeng sa tataiso ea litsebi,iteanye le ronabakeng sa litharollo tse khethehileng.
Ho nepahala le ho nepahala ho Pipette Tips
Bohlokoa ba Moralo oa Keletso bakeng sa Litekanyo tse Nepahetseng
Moralo oa malebela a pipette o phetha karolo ea bohlokoa ho netefatsa litekanyo tse nepahetseng tsa mokelikeli. Malebela a entsoeng hampe a ka lebisa ho lutla, ho fana ka molumo o sa tsitsang, le tšilafalo. Ho fumana liphetho tse nepahetseng, o lokela ho batla malebela a nang le likarolo tse kang:
- Ho lekana ho sireletsehileng ho thibela likheo tsa moea le ho netefatsa phepelo e tsitsitseng.
- Lisebelisoa tsa Hydrophobic tse fokotsang ho bolokoa ha metsi le tahlehelo ea sampole.
- Kabo ea mangolo e hlakileng bakeng sa ho metha molumo ka nepo.
- Ho lumellana le pipette ea hau ho qoba ho se nepahale ho bakoang ke ho khomarela ho sa lokelang.
Litlhahiso tsa boleng bo phahameng ba pipette li etsoa ka mokhoa o nepahetseng ho fana ka ts'ebetso e ts'oanang. Ho khetha malebela ho bahlahisi ba nang le botumo bo botle ho tiisa ho khomarela litekanyetso tse tiileng tsa boleng, tse bohlokoa bakeng sa liphello tse ka tšeptjoang.
Karolo ea Fit le Tiiso ho Thibela ho Dutla
Ho lekana hantle le ho tiisa pakeng tsa pipette le ntlha ke tsa bohlokoa bakeng saho thibela ho dutla le ho boloka bonnete. Malebela a sa lokeleng hantle a ka baka tahlehelo ea lisampole, a hlahisa lipompong tsa moea, kapa a baka ho rotha. Litaba tsena li sitisa botšepehi ba liteko tsa hau.
Ho qoba mathata ana, kamehla khetha malebela a lumellanang hantle le pipette ea hau. Malebela a ikhethileng a moetsi hangata a fana ka tse loketseng hantle, empa malebela a bokahohle a etselitsoeng mefuta e mengata le ona a ka sebetsa ka katleho. Kamehla hlahloba li-O-rings le litiiso ho pipette ea hau ho netefatsa hore li lula li le boemong bo botle. Tiiso e sireletsehileng e tiisa ho fana ka molumo o tsitsitseng le ho thibela tšilafalo.
Kamoo Molumo oa Keletso o Amang Nepahetseng kateng
Matla a molumo oa malebela a pipette a ama ka ho toba ho nepahala ha bona. Malebela a molumo o tlase a loketse lits'ebetso tse hlokang litekanyo tse nyane, tse nepahetseng, joalo ka PCR. Malebela a bophahamo bo mahareng a fana ka tekano lipakeng tsa ho nepahala le bokhoni, ho etsa hore li tšoanelehe bakeng sa mesebetsi e akaretsang ea laboratori. Malebela a molumo o phahameng a sebetsana le bongata bo boholo ntle le ho tela ho nepahala, e leng ntho ea bohlokoa bakeng sa phetisetso ea metsi a mangata.
Bakeng sa litlhahlobo tsa bohlokoa, malebela a ho boloka chelete e tlase a molemo haholo. Malebela ana a fokotsa ho bolokoa ha metsi, ho etsa bonnete ba hore sampole eohle e fanoa ka nepo. Ho khetha molumo o nepahetseng oa keletso bakeng sa sesebelisoa sa hau ho ntlafatsa ho nepahala le ho fokotsa liphoso tsamaisong ea metsi.
Taolo ea Tšilafalo le Tšilafalo
Ke Hobane'ng ha Malebela a Sterile Pipette a le Bohlokoa bakeng sa Likopo tse Bohloko
Malebela a sterile pipette a bohlokoa bakeng sa ho boloka maemo a aseptic ka har'a phallo ea mosebetsi ea laboratori e hlokolosi. Ho sebelisa malebela a sa hloekang ho ka lebisa likotsing tse 'maloa tsa tšoaetso:
- Tšilafalo ea Pipette-to-sample e etsahala ha pipette kapa tip e hlahisa litšila ka har'a sampuli.
- Tšilafalo ea sampole-to-pipette e etsahala ha mokelikeli o kena 'meleng oa pipette, o senya ho hloekisoa ha oona.
- Tšilafalo ea lisampole ho isa ho lisampole e hlaha ha mokelikeli o setseng ho tloha sampuling e 'ngoe ho ea ho o mong.
Ho qoba mathata ana, o tlameha ho sebelisa malebela a sterile pipette, haholo-holo bakeng sa lits'ebetso tse kang biology ea molek'hule kapa microbiology. Litlhahiso tse entsoeng pele ho sterilized tse tsoang ho bahlahisi ba netefalitsoeng li netefatsa ho thibela likokoana-hloko hantle le ho felisa likotsi tsa tšoaetso. Ho feta moo, malebela a boleng bo holimo a virgin polypropylene a fokotsa monyetla oa ho hlakoloa ho ama lisampole tsa hau.
Melemo ea ho Sebelisa Malebela a Filtered Pipette
Litlhahiso tsa pipette tse tlhotliloengfana ka karolo e eketsehileng ea tšireletso bakeng sa liteko tsa hau. Litlhahiso tsena li thibela li-aerosols hore li se ke tsa kena pipette, li sireletsa pipette le sampuli hore li se ke tsa silafala. Karolo ena e bohlokoa haholo lits'ebetsong tse hlokolosi joalo ka PCR kapa DNA tatellano, moo le litšila tse nyane li ka senyang sephetho.
Malebela a tlhotliloeng a boetse a boloka bohloeki ba mohlala ka ho thibela tšilafalo pakeng tsa lisampole. Ts'ebetso ea bona e phahameng ea baktheria (ho fihla ho 99%) e tiisa liphello tse ka tšeptjoang. Bakeng sa bafuputsi ba qalang, malebela ana a fokotsa kotsi ea ho qhalana ka phoso kapa tšilafalo, e leng se etsang hore e be khetho e sebetsang ea ho boloka botšepehi ba liteko.
Nako ea ho Khetha Malebela a Pipette a Sebeletsoeng Pele
Malebela a pele a sterilized pipette a bohlokoa haholo bakeng sa liteko tse kenyelletsang lik'hemik'hale tse bobebe kapa disampole tsa baeloji. Litlhahiso tsena li sireletsa pipette le sampole ho tsoa tšilafalo. Kee netefalitsoeng ntle le DNA, RNase, le endotoxins, e netefatsang boemo bo phahameng ka ho fetisisa ba sterility.
U lokela ho khetha malebela a pele a hloekisoang ha u sebetsa ho baeloji ea limolek'hule, microbiology, kapa ts'ebeliso efe kapa efe e hlokang maemo a se nang tšoaetso. Malebela ana a felisa tlhokeho ea autoclaving, ho boloka nako le ho netefatsa sterility e tsitsitseng. Tšebeliso ea bona e fokotsa likotsi tsa tšoaetso ea likokoana-hloko, e ba etsa khetho e tšepahalang bakeng sa mesebetsi ea bohlokoa ea laboratori.
Boleng ba lintho tse bonahalang le ho tšoarella ha Malebela a Pipette
Melemo ea Virgin Polypropylene in Tip Manufacturing
Tšebeliso eapolypropylene e sa tsoakoangtlhahisong ea pipette tip e tiisa boleng bo holimo le bohloeki. Boitsebiso bona bo fana ka melemo e mengata:
- E tlosa litšila tse bonoang joalo ka DNase, RNase, le li-pyrogens, tse ka senyang liteko tse hlokolosi.
- Li-protocol tse thata tsa ho hloekisa nakong ea tlhahiso li matlafatsa bohloeki ba malebela, li etsa hore li tšoanelehe bakeng sa lits'ebetso tsa bohlokoa.
- Virgin polypropylene e tlatsana le lits'ebetso tsa sterilization, joalo ka radiation, ho fokotsa likotsi tsa tšoaetso ea likokoana-hloko.
Ka ho khetha malebela a pipette a entsoeng ka virgin polypropylene, o netefatsa ts'ebetso e tšepahalang le ho boloka botšepehi ba lisampole tsa hau.
Malebela a ho Boloka Ha Tlase Bakeng sa ho Sebelisa Liquids tsa Viscous
Litlhahiso tsa pipette tsa ho boloka tlaase li bohlokoa ha u sebetsa ka lintho tse bonahalang tse kang li-enzyme kapa li-reagents. Litlhahiso tsena li na le sebaka se ka hare sa hydrophobic se fokotsang ho khomarela metsi. Moralo ona o fokotsa tahlehelo ea sampole mme o netefatsa phetiso e nepahetseng ea mokelikeli nakong ea liteko.
U rua molemo ka ho nepahala ho ntlafetseng le ho tšepahala ha u sebelisa malebela a ho boloka chelete e tlaase. Li sebetsa hantle haholo bakeng sa lits'ebetso tse hlokang ho ts'oaroa ka mokhoa o hlakileng oa lino tse khomarelang kapa tse teteaneng. Ka ho fokotsa litšila le ho ntlafatsa ho nepahala, malebela ana a tsamaisa mosebetsi oa hau le ho ntlafatsa liphetho tsa liteko.
Likhetho tse tšoarellang le tse ka sebelisoang hape bakeng sa Li-Labs tsa Eco-Friendly
Malebela a tsitsitseng a pipette a thusa ho fokotsa tšusumetso ea tikoloho ea ts'ebetso ea laboratori. Nahana ka lintlha tse latelang:
| Tšusumetso ea Tikoloho | Tlhaloso |
|---|---|
| Litšila tsa Plastiki | Ho feta liponto tse limilione tse likete tse 12 tsa litšila tsa polasetiki tse hlahisoang selemo le selemo ho tsoa ho lipolasetiki tse sebelisoang hang ka laboratoring. |
| Likhahla tsa CO2 | Tlhahiso ea 96-rack e le 'ngoe ea malebela a pipette ea polypropylene e lokolla hoo e ka bang 0.304 kg ea CO2 e lekanang. |
| Tšebeliso ea Metsi | E hloka lilithara tse ka bang 6.6 tsa metsi ho hlahisa malebela a 96-rack a pipette. |
Ho sebelisa hape malebela a pipette ke tharollo e sebetsang. Lithuto tsa netefatso ho tsoa mekhatlong e kang NIH le CDC li tiisa hore malebela a hlatsoitsoeng a boloka boleng bo ts'oanang le bo bocha. Mohlala, lab e sebelisang keletso ka 'ngoe makhetlo a leshome e ka fokotsa likhase tsa CO2 ka lithane tse 5.3 ka selemo. Ka ho sebelisa mekhoa e ka sebelisoang hape kapa e ka sebelisoang hape, o kenya letsoho tikolohong ea laboratori e tala ntle le ho senya ts'ebetso.
Ho lumellana le Ergonomics ea Litlhahiso tsa Pipette
Ho etsa bonnete ba ho lumellana le Mefuta e fapaneng ea Pipette
Ho khetha malebela a pipettetse lumellanang le pipette ea hau ke tsa bohlokoa bakeng sa ho fumana liphello tse nepahetseng le tse ka tšeptjoang. Ho se lumellane ho ka lebisa ho se lekane hantle, ho lutla, kapa ho fana ka molumo o fosahetseng. Ho etsa bonnete ba ho lumellana, nahana ka lintlha tse latelang:
- Bapisa malebela le moralo oa pipette ea hau ho qoba liphoso.
- Sebelisa lisebelisoa tsa boleng bo holimo ho thibela tšoaetso.
- Khetha malebela a fihlelang litlhoko tsa bophahamo ba liteko tsa hau.
- Khetha lipakeng tsa meralo e tloaelehileng kapa e sa bolokehang haholo ho latela ts'ebeliso ea hau.
- Batla mangolo a hlakileng bakeng sa litekanyo tse nepahetseng.
- Khetha malebela ho tsoa ho bahlahisi ba nang le litifikeiti tsa boleng.
- Leka-lekanya boleng le ditjeho ho etsa bonnete ba hore o sebetsa hantle ka ditshenyehelo.
Ka ho hlahloba lintlha tsena, u ka khetha malebela a pipette a ntlafatsang mosebetsi oa hau le ho boloka botšepehi ba liteko tsa hau.
Meetso ea Ergonomic bakeng sa Matšeliso a Basebelisi le Katleho
Litlhahiso tsa ergonomic pipettentlafatsa boiketlo ba basebedisi le katleho, haholo-holo nakong ea ho pheta-pheta mesebetsi. Litlhahiso tse khutšoanyane tsa pipette, ka mohlala, li u lumella ho pipette haufi le benche ha u sebetsa ka lipoleiti tse nang le li-multi-well. Moralo ona o fokotsa khatello ea letsoho mme o ntlafatsa boiketlo nakong ea tšebeliso ea nako e telele. Li-pipette tse bobebe le tsa ergonomic li fokotsa mokhathala oa matsoho, ho netefatsa boiphihlelo bo nepahetseng ba mosebelisi. Likarolo tsena ha li ntlafatse ho nepahala feela empa li boetse li ntlafatsa katleho ea laboratori ka kakaretso. Ho beha ergonomics pele ho malebela a hau a pipette ho ka ntlafatsa haholo tlhahiso ea hau le ho fokotsa khatello ea 'mele.
Ho bapisa Universal le Manufacturer-Specific Pipette Tips
Ho khetha pakeng tsa malebela a pipette a bokahohleng le a khethehileng a moetsi ho itšetlehile ka litlhoko tsa hau tse khethehileng. Tafole e ka tlase e totobatsa melemo le maikutlo a khetho ka 'ngoe:
| Mofuta oa Keletso | Melemo | Ho nahanela |
|---|---|---|
| Litlhahiso tsa Universal Pipette | E fapa-fapaneng ebile e bonolo; e ka sebelisoa le li-micropipettes tse fapaneng. | E kanna ea se fane ka ho lekana hantle bakeng sa li-micropipettes tsohle, tse amang ho nepahala. |
| Malebela a Khethehileng a Moetsi | E netefatsa ts'ebetso e bolokehileng le ts'ebetso e nepahetseng, e ntlafatsa ho nepahala. | Ka tloaelo e theko e boima haholo 'me e ka hloka ho fumana lits'ebeletso ho tsoa ho barekisi ba itseng. |
Malebela a Universal a fana ka maemo a bonolo, athe malebela a khethehileng a moetsi a fana ka mokhoa o nepahetseng. Lekola lintho tse tlang pele bophelong ba hau ho etsa khetho e nepahetseng bakeng sa litlhoko tsa laboratori ea hau.
Litlhahiso tsa boleng bo phahameng ba pipette li bohlokoa bakeng sa ho finyella liphello tse nepahetseng le tse se nang tšoaetso mosebetsing oa laboratori. U lokela ho etelletsa pele likarolo tse kang boleng ba thepa, sterility, le ho lumellana le pipette ea hau. Bakeng sa lits'ebetso tse hlokolosi, malebela a hloekisitsoeng le a tlhotliloeng a thibela tšilafalo le ho sireletsa botšepehi ba sampole. Kamehla amahanya bophahamo ba modumo le moralo ho latela litlhoko tsa hau tse ikhethileng ho netefatsa ts'ebetso e nepahetseng.
Nako ea poso: Feb-12-2025