Amasaha angahe mucyumweru utakaza amasahani yimbitse?
Urugamba ni ukuri.Nubwo imiyoboro myinshi cyangwa amasahani wapakiye mubushakashatsi bwawe cyangwa akazi, ubwenge bwawe burashobora gutangira kugukinisha mugihe cyo gupakira isahani 96 iteye ubwoba.
Biroroshye cyane kongeramo amajwi kumurongo utari mwiza cyangwa umurongo utari wo.Nibyoroshye cyane kubwimpanuka gukuba kabiri isahani yimbitse.
Cyangwa ukapakira icyitegererezo cyose nabi mumariba menshi, bigutwara amasaha yakazi.
Cyangwa, birashoboka ko wakoze byose neza, ariko utangira ubwa kabiri ukeka wenyine.Gutangira.
Igihe cyawe gifite agaciro cyane.Reagent yawe ifite agaciro gakomeye.Kandi, icy'ingenzi, amakuru yawe afite agaciro gakomeye.
Ntabwo tugomba kukubwira icyo guta igihe ibi, mugihe mubisanzwe ugomba gukora reagent hanyuma ukavanga.Byongeye, ntabwo byunvikana cyane kurwego rwicyizere.
Hano hari inama nziza nuburiganya kubandi ushobora gutangira kwinjiza muri gahunda yawe ya laboratoire.
Isahani 96 yimbitse ni iki?
Ikintu gikunze kwirengagizwa muri laboratoire hamwe nubushakashatsi ahantu hose, amasahani yimbitse meza kububiko bwigihe gito nigihe kirekire cyo kubika, gutegura, no kuvanga.Bashobora kugira kare kare cyangwa kuzenguruka hepfo.
Imikoreshereze yabo iratandukanye, ariko ikoreshwa kenshi mubuzima bwa siyanse yubuzima no gukoresha ubushakashatsi, harimo:
- Tissue umuco wumudugudu ukora nisesengura ryakagari
- Enzyme
- Inyigisho za proteomics
- Ibigega byongeye
- Ububiko bw'icyitegererezo butekanye (harimo ububiko bwa cryogenic)
Inama zo hejuru & amayeri yo gutsinda amakosa 96 yimbitse
Twakoze urutonde rwa sisitemu yo hejuru hamwe nuburyo bwa bagenzi bawe:
- Reba imitekerereze yawe kandi ukomeze kwibanda:Nkibintu byose mubuzima, amakosa akunda kubaho mugihe unaniwe, uhangayitse, cyangwa urangaye (… cyangwa byose byavuzwe haruguru).Reka guhangayikishwa no kwihuta binyuze mu nshingano zawe.Genda gahoro, hanyuma utekereze kuri buri ntambwe witonze.Kandi komeza kwibanda.Kuvuga no gukora bituma imirimo imwe igenda yihuta, ariko ntabwo iri hamwe niki gikorwa.Abashakashatsi bamwe bamanika "Nta kuvuga" biyandikisha kuko bari hagati yiki gikorwa.Kuruhura umuziki (cyane cyane ibikoresho), ariko, birashishikarizwa niba ukeneye urusaku rwinyuma mugihe ukora!
- Huza inama zawe za pipette kumariba ahuye:Agasanduku gashya ni byiza kubisahani byimbitse.Huza iriba nagasanduku uko ugenda.Gira agasanduku k'ibikubiyemo kuri standby mugihe urangije, ntugomba rero guhungabanya sisitemu yawe niba ukeneye byinshi.Koresha inama ya pipette kugirango ukurikirane neza kubara.
- Andika:Kora urupapuro rwa Excel kugirango uvange neza, hamwe namakarita 96 yimbitse.Buri riba rifite izina rya primers na sample.Shiraho shobuja yose avanze muburyo bwumvikana, na code yamabara kuri buri primer yashizeho (niba ukoresha ibirenze imwe).Zana iyi mpapuro nawe muri laboratoire, hanyuma urebe akamenyetso kurupapuro uko ugenda.Urashobora kandi kwandika umubare wa reagent kuri post-it hanyuma ukayigumana iruhande rwawe nkurufunguzo rwicyitegererezo nkuko uremereye.Toranya sisitemu yo gukora binyuze muri bo (urugero mubisobanuro cyangwa mubare, ukurikije uko byanditse) kandi ntuzigere utandukana na sisitemu.Mugihe ukora imvange, shyira ibintu byose murutonde rwawe, hanyuma ubimure mumfuruka ya kure iyo birangiye.
- Tape ninshuti yawe nshyashya:Kuraho isahani yuzuye, usibye agace urimo gupakira.Kora hejuru yisahani murubu buryo, kwimura kaseti buri gihe igice cyuzuye.Urashobora kuranga kaseti yawe (urugero A - H, 1 - 12) kugirango igufashe kuguma kumurongo.
Kurugero, mugihe urimo gupakira Gene A igishushanyo mbonera cyinkingi ya 1 nicya 2 yisahani yawe yimbitse, banza ufate kaseti hanyuma witwikire witonze inkingi 3 na 4. Urashobora no gukora iyi nkingi imwe icyarimwe, kugirango ugume kuri gahunda.Ifasha kuguma mu cyerekezo mugihe gikomeye cyo hagati.Gusa wibuke gufata isahani hasi ushikamye mugihe ukuyemo kaseti yawe, kugirango wirinde kumeneka. - Komera kuri yo:Niba ubonye sisitemu yawe idakora, ntuyihindure hagati.Hindura mbere cyangwa nyuma, ariko ntuzigere unyura hagati (biganisha ku rujijo rwinshi!).
- Imyitozo:Komeza guhuza inzira wahisemo.Kwiyemeza izi ntambwe kugirango wibuke imitsi bizatwara igihe, ariko mugihe kirenzeho ugomba gutangira kubona iterambere ryinshi mubikorwa byawe (kandi bikagabanuka cyane kumurimo wawe!)
Hitamo ibikoresho byiza:
Kuva mubikoresho kugeza ubuziranenge, amariba azengurutse cyangwa hepfo ya conical, hari amahitamo atandukanye mugihe utumije isahani yimbitse 96.
Bimwe mubitekerezo harimo:
- Ibikoresho: Ni izihe ngero ukoresha?Ese iriba ryanyu rikeneye gukenera lobind cyangwa silikonike?
- Ingano: Ni bangahe bikenera guhuza iriba ryimbitse 96 isahani ya PCR?
- Ubushyuhe: Ni ubuhe bushyuhe amariba yawe yimbitse akeneye kwihanganira?
- Ni izihe mbaraga za centrifugation zishobora isahani yawe 96 yimbitse?
Dore ibyo abahanga benshi bakoresha mubikorwa rusange:
Ibi byoroshye 96 Ibyapa Byimbitse
Uburyo aya masahani yimbitse afasha laboratoire n'abayobozi ba laboratoire:
- Aninzira yoroshyegukusanya no gutegura ingero (kubera ko ntihabuze ibyo bintu bibera muri laboratoire yawe burimunsi)
- Subiza umwanya wa labspace inyuma, hamwe nubushobozi bukomeye bwo gutondeka byoroshye kubika kuruta mbere hose
- Irinde kumeneka hamwekuvanga nezaby'amazi mato mato
- Igishushanyoigabanya kugumana inkuta, bityo ugasesagura bike byicyitegererezo cyawe
- Kwishura33% munsikurenza uko wifuza kubindi birango biyobora
Ibiranga harimo:
- Hasi
- Irashobora gukonjeshwa cyangwa gukonjeshwa (kugeza kuri -80 C)
- Guhagarara - ntibazabyitwaramo mumashanyarazi
- Shyiramo nta byuma biremereye kugirango bitezimbere neza
- Yashizweho ukurikije ubunini mpuzamahanga (SBS), bigatuma bukorerwa ahakorerwa
- Emera kugumana amazi make ya sample yawe kurukuta
Guhitamo isahani iburyo irashobora kugufasha kwirinda:
- Ingingo zabuze
- Icyitegererezo
- Buhoro buhoro akazi
- Igihe ntarengwa cyumushinga
Ubushakashatsi bwiza
Isahani 96 yimbitse iboneka muri laboratoire no mubigo byubushakashatsi ku isi.Barashobora kubika umwanya, imbaraga, hamwe nububiko, ariko sisitemu ikwiye ningirakamaro mugihe urangije akazi kawe.
Kuva mubushobozi bwububiko bwiyongereye, kugeza kuvangavanze, isahani yimbitse nibyiza kuri chimie ikomatanya hamwe nibisomero, birwanya imiti myinshi, ibishishwa, na alcool ikoreshwa muri chimie ikomatanya.
Icyitegererezo cyo gukusanya icyitegererezo, gutegura icyitegererezo, hamwe nigihe kirekire (cyangwa kigufi) ububiko bw'icyitegererezo, amasahani yimbitse hamwe na matelas yo gufunga bishobora guteza imbere akazi, kandi isahani iburyo yimbitse nayo izagufasha kubyara amakuru yujuje ubuziranenge kubikorwa bisanzwe muri ubumenyi bwubuzima (no hanze yarwo).
Igihe cyo kohereza: Gicurasi-10-2022